Bacterial Dispersers along Preferential Flow Paths of a Clay Till Depth Profile

Research output: Contribution to journalJournal articlepeer-review

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Bacterial Dispersers along Preferential Flow Paths of a Clay Till Depth Profile. / Krüger, U S; Dechesne, A; Bak, F; Badawi, N; Nybroe, O; Aamand, J.

In: Applied and Environmental Microbiology, Vol. 85, No. 6, e02658-18, 15.03.2019, p. 1-16.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Krüger, US, Dechesne, A, Bak, F, Badawi, N, Nybroe, O & Aamand, J 2019, 'Bacterial Dispersers along Preferential Flow Paths of a Clay Till Depth Profile', Applied and Environmental Microbiology, vol. 85, no. 6, e02658-18, pp. 1-16. https://doi.org/10.1128/AEM.02658-18

APA

Krüger, U. S., Dechesne, A., Bak, F., Badawi, N., Nybroe, O., & Aamand, J. (2019). Bacterial Dispersers along Preferential Flow Paths of a Clay Till Depth Profile. Applied and Environmental Microbiology, 85(6), 1-16. [e02658-18]. https://doi.org/10.1128/AEM.02658-18

Vancouver

Krüger US, Dechesne A, Bak F, Badawi N, Nybroe O, Aamand J. Bacterial Dispersers along Preferential Flow Paths of a Clay Till Depth Profile. Applied and Environmental Microbiology. 2019 Mar 15;85(6):1-16. e02658-18. https://doi.org/10.1128/AEM.02658-18

Author

Krüger, U S ; Dechesne, A ; Bak, F ; Badawi, N ; Nybroe, O ; Aamand, J. / Bacterial Dispersers along Preferential Flow Paths of a Clay Till Depth Profile. In: Applied and Environmental Microbiology. 2019 ; Vol. 85, No. 6. pp. 1-16.

Bibtex

@article{c5026ee2749944a8ab2a65887a70c360,
title = "Bacterial Dispersers along Preferential Flow Paths of a Clay Till Depth Profile",
abstract = "This study assessed the dispersal of five bacterial communities from contrasting compartments along a fractured clay till depth profile comprising plow layer soil, preferential flow paths (biopores and the tectonic fractures below), and matrix sediments, down to 350 cm below the surface. A recently developed expansion of the porous surface model (PSM) was used to capture bacterial communities dispersing under controlled hydration conditions on a soil-like surface. All five communities contained bacteria capable of active dispersal under relatively low hydration conditions (-3.1 kPa). Further testing of the plow layer community revealed active dispersal even at matric potentials of -6.3 to -8.4 kPa, previously thought to be too dry for dispersal on the PSM. Using 16S rRNA gene amplicon sequencing, the dispersing communities were found to be less diverse than their corresponding total communities. The dominant dispersers in most compartments belonged to the genus Pseudomonas and, in the plow layer soil, to Rahnella as well. An exception to this was the dispersing community in the matrix at 350 cm below the surface, which was dominated by Pantoea Hydrologically connected compartments shared proportionally more dispersing than nondispersing amplicon sequence variants (ASVs), suggesting that active dispersal is important for colonizing these compartments. These results highlight the importance of including soil profile heterogeneity when assessing the role of active dispersal and contribute to discerning the importance of active dispersal in the soil environment.IMPORTANCE The ability to disperse is considered essential for soil bacteria colonization and survival, yet very little is known about the dispersal ability of communities from different heterogeneous soil compartments. Important factors for dispersal are the thickness and connectivity of the liquid film between soil particles. The present results from a fractured clay till depth profile suggest that dispersal ability is common in various soil compartments and that most are dominated by a few dispersing taxa. Importantly, an increase in shared dispersers among the preferential flow paths of the clay till suggests that active dispersal plays a role in the successful colonization of these habitats.",
author = "Kr{\"u}ger, {U S} and A Dechesne and F Bak and N Badawi and O Nybroe and J Aamand",
note = "Copyright {\textcopyright} 2019 Kr{\"u}ger et al.",
year = "2019",
month = mar,
day = "15",
doi = "10.1128/AEM.02658-18",
language = "English",
volume = "85",
pages = "1--16",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "6",

}

RIS

TY - JOUR

T1 - Bacterial Dispersers along Preferential Flow Paths of a Clay Till Depth Profile

AU - Krüger, U S

AU - Dechesne, A

AU - Bak, F

AU - Badawi, N

AU - Nybroe, O

AU - Aamand, J

N1 - Copyright © 2019 Krüger et al.

PY - 2019/3/15

Y1 - 2019/3/15

N2 - This study assessed the dispersal of five bacterial communities from contrasting compartments along a fractured clay till depth profile comprising plow layer soil, preferential flow paths (biopores and the tectonic fractures below), and matrix sediments, down to 350 cm below the surface. A recently developed expansion of the porous surface model (PSM) was used to capture bacterial communities dispersing under controlled hydration conditions on a soil-like surface. All five communities contained bacteria capable of active dispersal under relatively low hydration conditions (-3.1 kPa). Further testing of the plow layer community revealed active dispersal even at matric potentials of -6.3 to -8.4 kPa, previously thought to be too dry for dispersal on the PSM. Using 16S rRNA gene amplicon sequencing, the dispersing communities were found to be less diverse than their corresponding total communities. The dominant dispersers in most compartments belonged to the genus Pseudomonas and, in the plow layer soil, to Rahnella as well. An exception to this was the dispersing community in the matrix at 350 cm below the surface, which was dominated by Pantoea Hydrologically connected compartments shared proportionally more dispersing than nondispersing amplicon sequence variants (ASVs), suggesting that active dispersal is important for colonizing these compartments. These results highlight the importance of including soil profile heterogeneity when assessing the role of active dispersal and contribute to discerning the importance of active dispersal in the soil environment.IMPORTANCE The ability to disperse is considered essential for soil bacteria colonization and survival, yet very little is known about the dispersal ability of communities from different heterogeneous soil compartments. Important factors for dispersal are the thickness and connectivity of the liquid film between soil particles. The present results from a fractured clay till depth profile suggest that dispersal ability is common in various soil compartments and that most are dominated by a few dispersing taxa. Importantly, an increase in shared dispersers among the preferential flow paths of the clay till suggests that active dispersal plays a role in the successful colonization of these habitats.

AB - This study assessed the dispersal of five bacterial communities from contrasting compartments along a fractured clay till depth profile comprising plow layer soil, preferential flow paths (biopores and the tectonic fractures below), and matrix sediments, down to 350 cm below the surface. A recently developed expansion of the porous surface model (PSM) was used to capture bacterial communities dispersing under controlled hydration conditions on a soil-like surface. All five communities contained bacteria capable of active dispersal under relatively low hydration conditions (-3.1 kPa). Further testing of the plow layer community revealed active dispersal even at matric potentials of -6.3 to -8.4 kPa, previously thought to be too dry for dispersal on the PSM. Using 16S rRNA gene amplicon sequencing, the dispersing communities were found to be less diverse than their corresponding total communities. The dominant dispersers in most compartments belonged to the genus Pseudomonas and, in the plow layer soil, to Rahnella as well. An exception to this was the dispersing community in the matrix at 350 cm below the surface, which was dominated by Pantoea Hydrologically connected compartments shared proportionally more dispersing than nondispersing amplicon sequence variants (ASVs), suggesting that active dispersal is important for colonizing these compartments. These results highlight the importance of including soil profile heterogeneity when assessing the role of active dispersal and contribute to discerning the importance of active dispersal in the soil environment.IMPORTANCE The ability to disperse is considered essential for soil bacteria colonization and survival, yet very little is known about the dispersal ability of communities from different heterogeneous soil compartments. Important factors for dispersal are the thickness and connectivity of the liquid film between soil particles. The present results from a fractured clay till depth profile suggest that dispersal ability is common in various soil compartments and that most are dominated by a few dispersing taxa. Importantly, an increase in shared dispersers among the preferential flow paths of the clay till suggests that active dispersal plays a role in the successful colonization of these habitats.

U2 - 10.1128/AEM.02658-18

DO - 10.1128/AEM.02658-18

M3 - Journal article

C2 - 30658975

VL - 85

SP - 1

EP - 16

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 6

M1 - e02658-18

ER -

ID: 215870624