Automated extraction of DNA and PCR setup using a Tecan Freedom EVO® liquid handler

Automated extraction of DNA and PCR setup using a Tecan Freedom EVO^® liquid handler

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Michael Stangegaard
Frøslev, Tobias Guldberg
Rune Frank-Hansen
Susan Laursen
Mads Jørgensen
Hansen, Anders Johannes
Morling, Niels

We have implemented and validated automated methods for DNA extraction and PCR setup developed for a Tecan Freedom EVO« liquid handler mounted with a Te-MagS(TM) magnetic separation device. The DNA was extracted using the Qiagen MagAttract« DNA Mini M48 kit. The DNA was amplified using AmpFlSTR« Identifiler«, Y-filer« (Applied Biosystems), GenePrint« FFFL and PowerPlex« Y (Promega). The methods were validated for fresh whole blood and blood from deceased according to EN/ISO 17025

Original language	English
Title of host publication	Progress in Forensic Genetics 13 : Proceedings from the 23rd International ISFG Congress
Editors	Niels Morling
Number of pages	3
Volume	E-Pub
Publisher	Elsevier
Publication date	2009
Pages	74-76
DOIs	https://doi.org/doi:10.1016/j.fsigss.2009.07.002
Publication status	Published - 2009
Event	23rd International ISFG Congress - Buenos Aires, Argentina Duration: 14 Sep 2009 → 18 Sep 2009

Conference

Conference	23rd International ISFG Congress
Land	Argentina
By	Buenos Aires
Periode	14/09/2009 → 18/09/2009

Series	Forensic Science International: Genetics Supplement Series
Number	1
Volume	2
ISSN	1875-1768

ID: 14439504