Autoantibodies to molecular targets in neutrophils in patients with ulcerative colitis

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Autoantibodies to molecular targets in neutrophils in patients with ulcerative colitis. / Brimnes, Jens; Nielsen, Ole H.; Wiik, Allan; Heegaard, Niels H.H.

In: Digestive Diseases and Sciences, Vol. 44, No. 2, 01.01.1999, p. 415-423.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Brimnes, J, Nielsen, OH, Wiik, A & Heegaard, NHH 1999, 'Autoantibodies to molecular targets in neutrophils in patients with ulcerative colitis', Digestive Diseases and Sciences, vol. 44, no. 2, pp. 415-423. https://doi.org/10.1023/A:1026683224057

APA

Brimnes, J., Nielsen, O. H., Wiik, A., & Heegaard, N. H. H. (1999). Autoantibodies to molecular targets in neutrophils in patients with ulcerative colitis. Digestive Diseases and Sciences, 44(2), 415-423. https://doi.org/10.1023/A:1026683224057

Vancouver

Brimnes J, Nielsen OH, Wiik A, Heegaard NHH. Autoantibodies to molecular targets in neutrophils in patients with ulcerative colitis. Digestive Diseases and Sciences. 1999 Jan 1;44(2):415-423. https://doi.org/10.1023/A:1026683224057

Author

Brimnes, Jens ; Nielsen, Ole H. ; Wiik, Allan ; Heegaard, Niels H.H. / Autoantibodies to molecular targets in neutrophils in patients with ulcerative colitis. In: Digestive Diseases and Sciences. 1999 ; Vol. 44, No. 2. pp. 415-423.

Bibtex

@article{fdf58b4361ee400894af7f056e20a338,
title = "Autoantibodies to molecular targets in neutrophils in patients with ulcerative colitis",
abstract = "Autoantibodies against neutrophil granulocytes are frequently observed in patients with ulcerative colitis, but a precise description of the autoantigens involved is lacking. Therefore, sera from 75 patients with ulcerative colitis were studied for antibody specificities by means of indirect immunofluorescence microscopy, enzyme-linked immunosorbent assays, and immunoblotting of extracts of neutrophils, neutrophil granules and lymphocytes. Fifty-six percent of the sera reacted in indirect immunofluorescence with ethanol-fixed neutrophils. On formalin-fixed cells most sera shifted fluorescence pattern, indicating a cytoplasmic origin of antigen(s). Only a few sera reacted with specific, known antigens. Immunoblots showed reactions with a broad panel of antigens with preferences for proteins around 55-65 kDa, which were present in azurophilic granules and in cytosol, an 80-kDa protein found in the specific granules, and a 110-kDa unknown cytosol component. In conclusion, neutrophil-specific IgG autoantibodies from ulcerative colitis patients react with several different antigens, most of which are of nonnuclear origin.",
keywords = "Anti-neutrophil cytoplasmic antibodies, Autoantigens, Neutrophils, Ulcerative colitis",
author = "Jens Brimnes and Nielsen, {Ole H.} and Allan Wiik and Heegaard, {Niels H.H.}",
year = "1999",
month = jan,
day = "1",
doi = "10.1023/A:1026683224057",
language = "English",
volume = "44",
pages = "415--423",
journal = "Digestive Diseases and Sciences",
issn = "0163-2116",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Autoantibodies to molecular targets in neutrophils in patients with ulcerative colitis

AU - Brimnes, Jens

AU - Nielsen, Ole H.

AU - Wiik, Allan

AU - Heegaard, Niels H.H.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Autoantibodies against neutrophil granulocytes are frequently observed in patients with ulcerative colitis, but a precise description of the autoantigens involved is lacking. Therefore, sera from 75 patients with ulcerative colitis were studied for antibody specificities by means of indirect immunofluorescence microscopy, enzyme-linked immunosorbent assays, and immunoblotting of extracts of neutrophils, neutrophil granules and lymphocytes. Fifty-six percent of the sera reacted in indirect immunofluorescence with ethanol-fixed neutrophils. On formalin-fixed cells most sera shifted fluorescence pattern, indicating a cytoplasmic origin of antigen(s). Only a few sera reacted with specific, known antigens. Immunoblots showed reactions with a broad panel of antigens with preferences for proteins around 55-65 kDa, which were present in azurophilic granules and in cytosol, an 80-kDa protein found in the specific granules, and a 110-kDa unknown cytosol component. In conclusion, neutrophil-specific IgG autoantibodies from ulcerative colitis patients react with several different antigens, most of which are of nonnuclear origin.

AB - Autoantibodies against neutrophil granulocytes are frequently observed in patients with ulcerative colitis, but a precise description of the autoantigens involved is lacking. Therefore, sera from 75 patients with ulcerative colitis were studied for antibody specificities by means of indirect immunofluorescence microscopy, enzyme-linked immunosorbent assays, and immunoblotting of extracts of neutrophils, neutrophil granules and lymphocytes. Fifty-six percent of the sera reacted in indirect immunofluorescence with ethanol-fixed neutrophils. On formalin-fixed cells most sera shifted fluorescence pattern, indicating a cytoplasmic origin of antigen(s). Only a few sera reacted with specific, known antigens. Immunoblots showed reactions with a broad panel of antigens with preferences for proteins around 55-65 kDa, which were present in azurophilic granules and in cytosol, an 80-kDa protein found in the specific granules, and a 110-kDa unknown cytosol component. In conclusion, neutrophil-specific IgG autoantibodies from ulcerative colitis patients react with several different antigens, most of which are of nonnuclear origin.

KW - Anti-neutrophil cytoplasmic antibodies

KW - Autoantigens

KW - Neutrophils

KW - Ulcerative colitis

UR - http://www.scopus.com/inward/record.url?scp=0032914819&partnerID=8YFLogxK

U2 - 10.1023/A:1026683224057

DO - 10.1023/A:1026683224057

M3 - Journal article

C2 - 10063932

AN - SCOPUS:0032914819

VL - 44

SP - 415

EP - 423

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 2

ER -

ID: 218717592