Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup. / Steffen, Jonas Hyld; Missel, Julie Winkel; Al-Jubair, Tamim; Kitchen, Philip; Salman, Mootaz M.; Bill, Roslyn M.; Törnroth-Horsefield, Susanna; Gourdon, Pontus.

In: STAR Protocols, Vol. 3, No. 2, 101312, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Steffen, JH, Missel, JW, Al-Jubair, T, Kitchen, P, Salman, MM, Bill, RM, Törnroth-Horsefield, S & Gourdon, P 2022, 'Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup', STAR Protocols, vol. 3, no. 2, 101312. https://doi.org/10.1016/j.xpro.2022.101312

APA

Steffen, J. H., Missel, J. W., Al-Jubair, T., Kitchen, P., Salman, M. M., Bill, R. M., Törnroth-Horsefield, S., & Gourdon, P. (2022). Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup. STAR Protocols, 3(2), [101312]. https://doi.org/10.1016/j.xpro.2022.101312

Vancouver

Steffen JH, Missel JW, Al-Jubair T, Kitchen P, Salman MM, Bill RM et al. Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup. STAR Protocols. 2022;3(2). 101312. https://doi.org/10.1016/j.xpro.2022.101312

Author

Steffen, Jonas Hyld ; Missel, Julie Winkel ; Al-Jubair, Tamim ; Kitchen, Philip ; Salman, Mootaz M. ; Bill, Roslyn M. ; Törnroth-Horsefield, Susanna ; Gourdon, Pontus. / Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup. In: STAR Protocols. 2022 ; Vol. 3, No. 2.

Bibtex

@article{1d92edbfb2424ca88d6b7abf7b45e7cf,

title = "Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup",

abstract = "Aquaporins (AQPs) are water channels embedded in the cell membrane that are critical in maintaining water homeostasis. We describe a protocol for determining the water permeation capacity of AQPs reconstituted into proteoliposomes. Using a stopped-flow setup, AQP embedded in proteoliposomes are exposed to an osmogenic gradient that triggers water flux. The consequent effects on proteoliposome size can be tracked using the fluorescence of an internalized fluorophore. This enables controlled characterization of water flux by AQPs. For complete details on the use and execution of this protocol, please refer to Kitchen et al. (2020).",

keywords = "Cell Membrane, Protein Biochemistry, Single-molecule Assays",

author = "Steffen, {Jonas Hyld} and Missel, {Julie Winkel} and Tamim Al-Jubair and Philip Kitchen and Salman, {Mootaz M.} and Bill, {Roslyn M.} and Susanna T{\"o}rnroth-Horsefield and Pontus Gourdon",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

doi = "10.1016/j.xpro.2022.101312",

language = "English",

volume = "3",

journal = "STAR Protocols",

issn = "2666-1667",

publisher = "Cell Press",

number = "2",

}

RIS

TY - JOUR

T1 - Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup

AU - Steffen, Jonas Hyld

AU - Missel, Julie Winkel

AU - Al-Jubair, Tamim

AU - Kitchen, Philip

AU - Salman, Mootaz M.

AU - Bill, Roslyn M.

AU - Törnroth-Horsefield, Susanna

AU - Gourdon, Pontus

PY - 2022

Y1 - 2022

N2 - Aquaporins (AQPs) are water channels embedded in the cell membrane that are critical in maintaining water homeostasis. We describe a protocol for determining the water permeation capacity of AQPs reconstituted into proteoliposomes. Using a stopped-flow setup, AQP embedded in proteoliposomes are exposed to an osmogenic gradient that triggers water flux. The consequent effects on proteoliposome size can be tracked using the fluorescence of an internalized fluorophore. This enables controlled characterization of water flux by AQPs. For complete details on the use and execution of this protocol, please refer to Kitchen et al. (2020).

AB - Aquaporins (AQPs) are water channels embedded in the cell membrane that are critical in maintaining water homeostasis. We describe a protocol for determining the water permeation capacity of AQPs reconstituted into proteoliposomes. Using a stopped-flow setup, AQP embedded in proteoliposomes are exposed to an osmogenic gradient that triggers water flux. The consequent effects on proteoliposome size can be tracked using the fluorescence of an internalized fluorophore. This enables controlled characterization of water flux by AQPs. For complete details on the use and execution of this protocol, please refer to Kitchen et al. (2020).

KW - Cell Membrane

KW - Protein Biochemistry

KW - Single-molecule Assays

UR - http://www.scopus.com/inward/record.url?scp=85128333189&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2022.101312

DO - 10.1016/j.xpro.2022.101312

M3 - Journal article

C2 - 35496800

AN - SCOPUS:85128333189

VL - 3

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 2

M1 - 101312

ER -

ID: 311124113