Applicability of Commercially Available ELISA Kits for the Quantification of Faecal Immunoreactive Corticosterone Metabolites in Mice

Research output: Contribution to journalJournal articlepeer-review

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Applicability of Commercially Available ELISA Kits for the Quantification of Faecal Immunoreactive Corticosterone Metabolites in Mice. / Abelson, Klas S P; Kalliokoski, Otto; Teilmann, Anne Charlotte; Hau, Jann.

In: In Vivo, Vol. 30, No. 6, 01.11.2016, p. 739-744.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Abelson, KSP, Kalliokoski, O, Teilmann, AC & Hau, J 2016, 'Applicability of Commercially Available ELISA Kits for the Quantification of Faecal Immunoreactive Corticosterone Metabolites in Mice', In Vivo, vol. 30, no. 6, pp. 739-744. https://doi.org/10.21873/invivo.10989

APA

Abelson, K. S. P., Kalliokoski, O., Teilmann, A. C., & Hau, J. (2016). Applicability of Commercially Available ELISA Kits for the Quantification of Faecal Immunoreactive Corticosterone Metabolites in Mice. In Vivo, 30(6), 739-744. https://doi.org/10.21873/invivo.10989

Vancouver

Abelson KSP, Kalliokoski O, Teilmann AC, Hau J. Applicability of Commercially Available ELISA Kits for the Quantification of Faecal Immunoreactive Corticosterone Metabolites in Mice. In Vivo. 2016 Nov 1;30(6):739-744. https://doi.org/10.21873/invivo.10989

Author

Abelson, Klas S P ; Kalliokoski, Otto ; Teilmann, Anne Charlotte ; Hau, Jann. / Applicability of Commercially Available ELISA Kits for the Quantification of Faecal Immunoreactive Corticosterone Metabolites in Mice. In: In Vivo. 2016 ; Vol. 30, No. 6. pp. 739-744.

Bibtex

@article{eda436055e4c4a89bc4d11130a609d25,
title = "Applicability of Commercially Available ELISA Kits for the Quantification of Faecal Immunoreactive Corticosterone Metabolites in Mice",
abstract = "Background: Commercially available ELISA kits are popular among investigators that quantify faecal corticosterone or cortisol metabolites (FCM) for stress assessment in animals. However, in faeces, these assays mainly detect immunoreactive glucocorticoid metabolites. Since different assays contain antibodies of different origin, the detection level and cross-reactivity towards different metabolites and other steroids differ considerably between assays. Thus, the validity of one assay for FCM quantification in stress assessment is not necessarily the same for another assay. Materials and Methods: The present study was designed to investigate corticosterone (CORT) in serum and FCM levels in faeces of laboratory mice, as quantified in four different ELISA kits (DRG EIA-4164, Demeditec DEV9922, Enzo ADI-900-097 and Cayman EIA kit 500655). Assay kits were chosen based on the origin of the antibody, detection level and variation in cross-reactivity. Results: As expected, all four assay kits could detect higher serum CORT levels in mice treated with adrenocorticotropic hormone (ACTH), compared to untreated mice. Unexpectedly though, the measured concentration of serum CORT differed significantly between assays, in both groups of mice. In faecal samples, there was no consistent positive correlation between the levels detected in the four assays and the measured concentration of FCM also differed between assays. Conclusion: Whereas commercially available CORT ELISAs are frequently successfully used for FCM quantification, validation of the assays is necessary as all assays do not work well under all circumstances. In this study, the ELISAs could determine relative differences in serum CORT levels and FCM levels between samples; however, the fidelity of the measurements to the true concentrations was low.",
keywords = "Faecal corticosterone metabolites, Immune assays, Mice, Stress",
author = "Abelson, {Klas S P} and Otto Kalliokoski and Teilmann, {Anne Charlotte} and Jann Hau",
year = "2016",
month = nov,
day = "1",
doi = "10.21873/invivo.10989",
language = "English",
volume = "30",
pages = "739--744",
journal = "In Vivo",
issn = "0258-851X",
publisher = "International Institute of Anticancer Research",
number = "6",

}

RIS

TY - JOUR

T1 - Applicability of Commercially Available ELISA Kits for the Quantification of Faecal Immunoreactive Corticosterone Metabolites in Mice

AU - Abelson, Klas S P

AU - Kalliokoski, Otto

AU - Teilmann, Anne Charlotte

AU - Hau, Jann

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Background: Commercially available ELISA kits are popular among investigators that quantify faecal corticosterone or cortisol metabolites (FCM) for stress assessment in animals. However, in faeces, these assays mainly detect immunoreactive glucocorticoid metabolites. Since different assays contain antibodies of different origin, the detection level and cross-reactivity towards different metabolites and other steroids differ considerably between assays. Thus, the validity of one assay for FCM quantification in stress assessment is not necessarily the same for another assay. Materials and Methods: The present study was designed to investigate corticosterone (CORT) in serum and FCM levels in faeces of laboratory mice, as quantified in four different ELISA kits (DRG EIA-4164, Demeditec DEV9922, Enzo ADI-900-097 and Cayman EIA kit 500655). Assay kits were chosen based on the origin of the antibody, detection level and variation in cross-reactivity. Results: As expected, all four assay kits could detect higher serum CORT levels in mice treated with adrenocorticotropic hormone (ACTH), compared to untreated mice. Unexpectedly though, the measured concentration of serum CORT differed significantly between assays, in both groups of mice. In faecal samples, there was no consistent positive correlation between the levels detected in the four assays and the measured concentration of FCM also differed between assays. Conclusion: Whereas commercially available CORT ELISAs are frequently successfully used for FCM quantification, validation of the assays is necessary as all assays do not work well under all circumstances. In this study, the ELISAs could determine relative differences in serum CORT levels and FCM levels between samples; however, the fidelity of the measurements to the true concentrations was low.

AB - Background: Commercially available ELISA kits are popular among investigators that quantify faecal corticosterone or cortisol metabolites (FCM) for stress assessment in animals. However, in faeces, these assays mainly detect immunoreactive glucocorticoid metabolites. Since different assays contain antibodies of different origin, the detection level and cross-reactivity towards different metabolites and other steroids differ considerably between assays. Thus, the validity of one assay for FCM quantification in stress assessment is not necessarily the same for another assay. Materials and Methods: The present study was designed to investigate corticosterone (CORT) in serum and FCM levels in faeces of laboratory mice, as quantified in four different ELISA kits (DRG EIA-4164, Demeditec DEV9922, Enzo ADI-900-097 and Cayman EIA kit 500655). Assay kits were chosen based on the origin of the antibody, detection level and variation in cross-reactivity. Results: As expected, all four assay kits could detect higher serum CORT levels in mice treated with adrenocorticotropic hormone (ACTH), compared to untreated mice. Unexpectedly though, the measured concentration of serum CORT differed significantly between assays, in both groups of mice. In faecal samples, there was no consistent positive correlation between the levels detected in the four assays and the measured concentration of FCM also differed between assays. Conclusion: Whereas commercially available CORT ELISAs are frequently successfully used for FCM quantification, validation of the assays is necessary as all assays do not work well under all circumstances. In this study, the ELISAs could determine relative differences in serum CORT levels and FCM levels between samples; however, the fidelity of the measurements to the true concentrations was low.

KW - Faecal corticosterone metabolites

KW - Immune assays

KW - Mice

KW - Stress

U2 - 10.21873/invivo.10989

DO - 10.21873/invivo.10989

M3 - Journal article

AN - SCOPUS:84994235549

VL - 30

SP - 739

EP - 744

JO - In Vivo

JF - In Vivo

SN - 0258-851X

IS - 6

ER -

ID: 171501201