Antagonism of the interleukin 4 receptor α promotes TH1-signalling among T cells from patients with atopic dermatitis after stimulation
Research output: Contribution to journal › Journal article › Research › peer-review
Atopic dermatitis (AD) is a chronic and relapsing inflammatory skin disease. Molecular characterization of AD shows an underlying inflammation with tissue infiltration of T helper (TH ) 2 cells and increased IL-4 and IL-13. The multifaceted roles of IL-4 and IL-13 in allergic disease development make IL-4Rα an attractive target for treatment strategies, and a neutralizing monoclonal antibody which antagonizes the effects of both IL-4 and IL-13 by blocking the interaction site found in the IL-4 receptor subunit α (IL-4Rα) has been successfully used to treat patients with moderate-to-severe AD. To elucidate the effects of IL-4Rα blockade on the cellular level, we used flow cytometry to examine cytokine production after antigen stimulation in human T cells from patients with AD (n = 12) and healthy controls (n = 6). The cells were stimulated with and without a neutralizing monoclonal antibody against IL-4Rα. Our results indicate that blocking IL-4Rα prohibits IL-4 signalling and IL-13 signalling and thereby TH 2 differentiation followed by an upregulation of interferon-γ-producing cells.
Original language | English |
---|---|
Article number | e12835 |
Journal | Scandinavian Journal of Immunology |
Volume | 91 |
Issue number | 1 |
Number of pages | 6 |
ISSN | 0300-9475 |
DOIs | |
Publication status | Published - 2020 |
- Adult, Biomarkers, Cytokines/metabolism, Dermatitis, Atopic/immunology, Female, Humans, Immunoglobulin E/blood, Interleukin-4 Receptor alpha Subunit/antagonists & inhibitors, Leukocytes, Mononuclear/immunology, Male, Middle Aged, Signal Transduction, T-Lymphocyte Subsets/immunology, Th1 Cells/immunology, Young Adult
Research areas
ID: 257035759