TY - JOUR

T1 - Analytical methods for determining urinary catecholamines in healthy subjects

AU - Nikolajsen, Rikke P.H.

AU - Hansen, Åse Marie

PY - 2001/12/10

Y1 - 2001/12/10

N2 - Objective: To perform a critical review of analytical methods for urinary catecholamines based on method performance parameters, such as analytical range, limit of detection (LOD), robustness, and the applicability for assessment of stress effects in urine from healthy subjects. Method: Five criteria were chosen for rating the analytical methods. The score value should give an indication of the usefulness of the method for measurement of catecholamines in urine from healthy subjects. The maximum score for a paper is '5', and one point is given for each of the following. 1. Analytical range must start at 7 nmol/l or below. 2. LOD (or limit of quantification (LOQ)) <7 nmol/l for both adrenaline (A) and noradrenaline (NA). 3. Some precision parameters have been described (i.e. repeatability and/or reproducibility). 4. Urine samples were analysed. 5. The method is fully automated method (i.e. clean-up procedure included in system). Results: A total of 7, out of 28 papers rated score the maximum of '5', and 5 papers score '4'; these are all high performance liquid chromatographic (HPLC) methods. The scores of the remaining 16 papers are distributed as follows: 10 papers score '3', 4 papers score '2' and 2 papers score '1'. Conclusion: The literature review has shown that automated HPLC systems still are the most applicable and well-documented methods for the analysis of A and NA in urine. Some quality parameters were summarised, and it was shown that many of the papers lack valuable information. It is recommended that future papers validate the method described and report the necessary quality parameters such as LOD, analytical range, precision, etc.

AB - Objective: To perform a critical review of analytical methods for urinary catecholamines based on method performance parameters, such as analytical range, limit of detection (LOD), robustness, and the applicability for assessment of stress effects in urine from healthy subjects. Method: Five criteria were chosen for rating the analytical methods. The score value should give an indication of the usefulness of the method for measurement of catecholamines in urine from healthy subjects. The maximum score for a paper is '5', and one point is given for each of the following. 1. Analytical range must start at 7 nmol/l or below. 2. LOD (or limit of quantification (LOQ)) <7 nmol/l for both adrenaline (A) and noradrenaline (NA). 3. Some precision parameters have been described (i.e. repeatability and/or reproducibility). 4. Urine samples were analysed. 5. The method is fully automated method (i.e. clean-up procedure included in system). Results: A total of 7, out of 28 papers rated score the maximum of '5', and 5 papers score '4'; these are all high performance liquid chromatographic (HPLC) methods. The scores of the remaining 16 papers are distributed as follows: 10 papers score '3', 4 papers score '2' and 2 papers score '1'. Conclusion: The literature review has shown that automated HPLC systems still are the most applicable and well-documented methods for the analysis of A and NA in urine. Some quality parameters were summarised, and it was shown that many of the papers lack valuable information. It is recommended that future papers validate the method described and report the necessary quality parameters such as LOD, analytical range, precision, etc.

KW - Analytical method

KW - Catecholamines

KW - Occupational health

KW - Review

KW - Urine

KW - Validation

UR - http://www.scopus.com/inward/record.url?scp=0035842416&partnerID=8YFLogxK

U2 - 10.1016/S0003-2670(01)01358-7

DO - 10.1016/S0003-2670(01)01358-7

M3 - Review

AN - SCOPUS:0035842416

VL - 449

SP - 1

EP - 15

JO - Vibrational Spectroscopy

JF - Vibrational Spectroscopy

SN - 0924-2031

IS - 1-2

ER -