Acetylation dynamics and stoichiometry in Saccharomyces cerevisiae
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Acetylation dynamics and stoichiometry in Saccharomyces cerevisiae. / Weinert, Brian Tate; Iesmantavicius, Vytautas; Moustafa, Tarek; Schölz, Christian; Wagner, Sebastian A; Magnes, Christoph; Zechner, Rudolf; Choudhary, Chuna Ram.
In: Molecular Systems Biology, Vol. 10, No. 1, 31.01.2014, p. 716.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Acetylation dynamics and stoichiometry in Saccharomyces cerevisiae
AU - Weinert, Brian Tate
AU - Iesmantavicius, Vytautas
AU - Moustafa, Tarek
AU - Schölz, Christian
AU - Wagner, Sebastian A
AU - Magnes, Christoph
AU - Zechner, Rudolf
AU - Choudhary, Chuna Ram
PY - 2014/1/31
Y1 - 2014/1/31
N2 - Lysine acetylation is a frequently occurring posttranslational modification; however, little is known about the origin and regulation of most sites. Here we used quantitative mass spectrometry to analyze acetylation dynamics and stoichiometry in Saccharomyces cerevisiae. We found that acetylation accumulated in growth-arrested cells in a manner that depended on acetyl-CoA generation in distinct subcellular compartments. Mitochondrial acetylation levels correlated with acetyl-CoA concentration in vivo and acetyl-CoA acetylated lysine residues nonenzymatically in vitro. We developed a method to estimate acetylation stoichiometry and found that the vast majority of mitochondrial and cytoplasmic acetylation had a very low stoichiometry. However, mitochondrial acetylation occurred at a significantly higher basal level than cytoplasmic acetylation, consistent with the distinct acetylation dynamics and higher acetyl-CoA concentration in mitochondria. High stoichiometry acetylation occurred mostly on histones, proteins present in histone acetyltransferase and deacetylase complexes, and on transcription factors. These data show that a majority of acetylation occurs at very low levels in exponentially growing yeast and is uniformly affected by exposure to acetyl-CoA.
AB - Lysine acetylation is a frequently occurring posttranslational modification; however, little is known about the origin and regulation of most sites. Here we used quantitative mass spectrometry to analyze acetylation dynamics and stoichiometry in Saccharomyces cerevisiae. We found that acetylation accumulated in growth-arrested cells in a manner that depended on acetyl-CoA generation in distinct subcellular compartments. Mitochondrial acetylation levels correlated with acetyl-CoA concentration in vivo and acetyl-CoA acetylated lysine residues nonenzymatically in vitro. We developed a method to estimate acetylation stoichiometry and found that the vast majority of mitochondrial and cytoplasmic acetylation had a very low stoichiometry. However, mitochondrial acetylation occurred at a significantly higher basal level than cytoplasmic acetylation, consistent with the distinct acetylation dynamics and higher acetyl-CoA concentration in mitochondria. High stoichiometry acetylation occurred mostly on histones, proteins present in histone acetyltransferase and deacetylase complexes, and on transcription factors. These data show that a majority of acetylation occurs at very low levels in exponentially growing yeast and is uniformly affected by exposure to acetyl-CoA.
U2 - 10.1002/msb.134766
DO - 10.1002/msb.134766
M3 - Journal article
C2 - 24489116
VL - 10
SP - 716
JO - Molecular Systems Biology
JF - Molecular Systems Biology
SN - 1744-4292
IS - 1
ER -
ID: 98145752