A synthetic consortium of 100 gut commensals modulates the composition and function in a colon model of the microbiome of elderly subjects

Research output: Contribution to journalJournal articlepeer-review

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A synthetic consortium of 100 gut commensals modulates the composition and function in a colon model of the microbiome of elderly subjects. / Perez, Marta; Ntemiri, Alexandra; Tan, Huizi; Harris, Hugh M B; Roager, Henrik Munch; Ribière, Céline; O'Toole, Paul W.

In: Gut Microbes, Vol. 13, No. 1, e1919464, 2021.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Perez, M, Ntemiri, A, Tan, H, Harris, HMB, Roager, HM, Ribière, C & O'Toole, PW 2021, 'A synthetic consortium of 100 gut commensals modulates the composition and function in a colon model of the microbiome of elderly subjects', Gut Microbes, vol. 13, no. 1, e1919464. https://doi.org/10.1080/19490976.2021.1919464

APA

Perez, M., Ntemiri, A., Tan, H., Harris, H. M. B., Roager, H. M., Ribière, C., & O'Toole, P. W. (2021). A synthetic consortium of 100 gut commensals modulates the composition and function in a colon model of the microbiome of elderly subjects. Gut Microbes, 13(1), [e1919464]. https://doi.org/10.1080/19490976.2021.1919464

Vancouver

Perez M, Ntemiri A, Tan H, Harris HMB, Roager HM, Ribière C et al. A synthetic consortium of 100 gut commensals modulates the composition and function in a colon model of the microbiome of elderly subjects. Gut Microbes. 2021;13(1). e1919464. https://doi.org/10.1080/19490976.2021.1919464

Author

Perez, Marta ; Ntemiri, Alexandra ; Tan, Huizi ; Harris, Hugh M B ; Roager, Henrik Munch ; Ribière, Céline ; O'Toole, Paul W. / A synthetic consortium of 100 gut commensals modulates the composition and function in a colon model of the microbiome of elderly subjects. In: Gut Microbes. 2021 ; Vol. 13, No. 1.

Bibtex

@article{e43245e33b6d4edf8005496cd8ffb5ca,
title = "A synthetic consortium of 100 gut commensals modulates the composition and function in a colon model of the microbiome of elderly subjects",
abstract = "Administration of cultured gut isolates holds promise for modulating the altered composition and function of the microbiota in older subjects, and for promoting their health. From among 692 initial isolates, we selected 100 gut commensal strains (MCC100) based on emulating the gut microbiota of healthy subjects, and retaining strain diversity within selected species. MCC100 susceptibility to seven antibiotics was determined, and their genomes were screened for virulence factor, antimicrobial resistance and bacteriocin genes. Supplementation of healthy and frail elderly microbiota types with the MCC100 in an in vitro colon model increased alpha-diversity, raised relative abundance of taxa including Blautia luti, Bacteroides fragilis, and Sutterella wadsworthensis; and introduced taxa such as Bifidobacterium spp. Microbiota changes correlated with higher levels of branched chain amino acids, which are health-associated in elderly. The study establishes that the MCC100 consortium can modulate older subjects' microbiota composition and associated metabolome in vitro, paving the way for pre-clinical and human trials.",
keywords = "Faculty of Science, Gut microbiome, Synthetic microbiome, Consortium, Antibiotics, Alpha-diversity, Elderly, Branched chain amino acids, BCAA",
author = "Marta Perez and Alexandra Ntemiri and Huizi Tan and Harris, {Hugh M B} and Roager, {Henrik Munch} and C{\'e}line Ribi{\`e}re and O'Toole, {Paul W}",
note = "CURIS 2021 NEXS 173",
year = "2021",
doi = "10.1080/19490976.2021.1919464",
language = "English",
volume = "13",
journal = "Gut Microbes",
issn = "1949-0976",
publisher = "Taylor & Francis",
number = "1",

}

RIS

TY - JOUR

T1 - A synthetic consortium of 100 gut commensals modulates the composition and function in a colon model of the microbiome of elderly subjects

AU - Perez, Marta

AU - Ntemiri, Alexandra

AU - Tan, Huizi

AU - Harris, Hugh M B

AU - Roager, Henrik Munch

AU - Ribière, Céline

AU - O'Toole, Paul W

N1 - CURIS 2021 NEXS 173

PY - 2021

Y1 - 2021

N2 - Administration of cultured gut isolates holds promise for modulating the altered composition and function of the microbiota in older subjects, and for promoting their health. From among 692 initial isolates, we selected 100 gut commensal strains (MCC100) based on emulating the gut microbiota of healthy subjects, and retaining strain diversity within selected species. MCC100 susceptibility to seven antibiotics was determined, and their genomes were screened for virulence factor, antimicrobial resistance and bacteriocin genes. Supplementation of healthy and frail elderly microbiota types with the MCC100 in an in vitro colon model increased alpha-diversity, raised relative abundance of taxa including Blautia luti, Bacteroides fragilis, and Sutterella wadsworthensis; and introduced taxa such as Bifidobacterium spp. Microbiota changes correlated with higher levels of branched chain amino acids, which are health-associated in elderly. The study establishes that the MCC100 consortium can modulate older subjects' microbiota composition and associated metabolome in vitro, paving the way for pre-clinical and human trials.

AB - Administration of cultured gut isolates holds promise for modulating the altered composition and function of the microbiota in older subjects, and for promoting their health. From among 692 initial isolates, we selected 100 gut commensal strains (MCC100) based on emulating the gut microbiota of healthy subjects, and retaining strain diversity within selected species. MCC100 susceptibility to seven antibiotics was determined, and their genomes were screened for virulence factor, antimicrobial resistance and bacteriocin genes. Supplementation of healthy and frail elderly microbiota types with the MCC100 in an in vitro colon model increased alpha-diversity, raised relative abundance of taxa including Blautia luti, Bacteroides fragilis, and Sutterella wadsworthensis; and introduced taxa such as Bifidobacterium spp. Microbiota changes correlated with higher levels of branched chain amino acids, which are health-associated in elderly. The study establishes that the MCC100 consortium can modulate older subjects' microbiota composition and associated metabolome in vitro, paving the way for pre-clinical and human trials.

KW - Faculty of Science

KW - Gut microbiome

KW - Synthetic microbiome

KW - Consortium

KW - Antibiotics

KW - Alpha-diversity

KW - Elderly

KW - Branched chain amino acids

KW - BCAA

U2 - 10.1080/19490976.2021.1919464

DO - 10.1080/19490976.2021.1919464

M3 - Journal article

C2 - 33993839

VL - 13

JO - Gut Microbes

JF - Gut Microbes

SN - 1949-0976

IS - 1

M1 - e1919464

ER -

ID: 269513066