A sensitive issue: Pyrosequencing as a valuable forensic SNP typing platform
Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review
Analysing minute amounts of DNA is a routine challenge in forensics in part due to the poor sensitivity of an instrument and its inability to detect results from forensic samples. In this study, the sensitivity of the Pyrosequencing method is investigated using varying concentrations of DNA and five autosomal single nucleotide polymorphisms in singleplex on both available instrument models; the PSQ(TM) 96MA and PSQ(TM) HS 96A. A detailed comparison of the two models was completed while establishing a lower limit of detection on both instruments to give results supporting the use of Pyrosequencing as a valuable forensic SNP typing platform
Original language | English |
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Title of host publication | Progress in Forensic Genetics 11 : Proceedings of the 21st International ISFG Congress |
Volume | 1288 |
Publisher | Elsevier |
Publication date | 2006 |
Pages | 52-54 |
DOIs | |
Publication status | Published - 2006 |
Event | 21st International ISFG Congress - Ponta Delgada, The Azores, Portugal Duration: 13 Sep 2005 → 16 Sep 2005 |
Conference
Conference | 21st International ISFG Congress |
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Land | Portugal |
By | Ponta Delgada, The Azores |
Periode | 13/09/2005 → 16/09/2005 |
Series | ICS - International Congress Series |
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ID: 18251564