A quantitative 14-3-3 interaction screen connects the nuclear exosome targeting complex to the DNA damage response

Research output: Contribution to journal › Journal article › Research › peer-review

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A quantitative 14-3-3 interaction screen connects the nuclear exosome targeting complex to the DNA damage response. / Blasius, Melanie; Wagner, Sebastian A; Choudhary, Chuna Ram; Bartek, Jiri; Jackson, Stephen P.

In: Genes & Development, Vol. 28, No. 18, 04.09.2014, p. 1977-1982.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Blasius, M, Wagner, SA, Choudhary, CR, Bartek, J & Jackson, SP 2014, 'A quantitative 14-3-3 interaction screen connects the nuclear exosome targeting complex to the DNA damage response', Genes & Development, vol. 28, no. 18, pp. 1977-1982. https://doi.org/10.1101/gad.246272.114

APA

Blasius, M., Wagner, S. A., Choudhary, C. R., Bartek, J., & Jackson, S. P. (2014). A quantitative 14-3-3 interaction screen connects the nuclear exosome targeting complex to the DNA damage response. Genes & Development, 28(18), 1977-1982. https://doi.org/10.1101/gad.246272.114

Vancouver

Blasius M, Wagner SA, Choudhary CR, Bartek J, Jackson SP. A quantitative 14-3-3 interaction screen connects the nuclear exosome targeting complex to the DNA damage response. Genes & Development. 2014 Sep 4;28(18):1977-1982. https://doi.org/10.1101/gad.246272.114

Author

Blasius, Melanie ; Wagner, Sebastian A ; Choudhary, Chuna Ram ; Bartek, Jiri ; Jackson, Stephen P. / A quantitative 14-3-3 interaction screen connects the nuclear exosome targeting complex to the DNA damage response. In: Genes & Development. 2014 ; Vol. 28, No. 18. pp. 1977-1982.

Bibtex

@article{57a78c15f5c04e2b91b67131c4b799b5,
title = "A quantitative 14-3-3 interaction screen connects the nuclear exosome targeting complex to the DNA damage response",
abstract = "RNA metabolism is altered following DNA damage, but the underlying mechanisms are not well understood. Through a 14-3-3 interaction screen for DNA damage-induced protein interactions in human cells, we identified protein complexes connected to RNA biology. These include the nuclear exosome targeting (NEXT) complex that regulates turnover of noncoding RNAs termed promoter upstream transcripts (PROMPTs). We show that the NEXT subunit RBM7 is phosphorylated upon DNA damage by the MAPKAPK2 kinase and establish that this mediates 14-3-3 binding and decreases PROMPT binding. These findings and our observation that cells lacking RBM7 display DNA damage hypersensitivity link PROMPT turnover to the DNA damage response.",
author = "Melanie Blasius and Wagner, {Sebastian A} and Choudhary, {Chuna Ram} and Jiri Bartek and Jackson, {Stephen P}",
note = "{\textcopyright} 2014 Blasius et al.; Published by Cold Spring Harbor Laboratory Press.",
year = "2014",
month = sep,
day = "4",
doi = "10.1101/gad.246272.114",
language = "English",
volume = "28",
pages = "1977--1982",
journal = "Genes & Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "18",

}

RIS

TY - JOUR

T1 - A quantitative 14-3-3 interaction screen connects the nuclear exosome targeting complex to the DNA damage response

AU - Blasius, Melanie

AU - Wagner, Sebastian A

AU - Choudhary, Chuna Ram

AU - Bartek, Jiri

AU - Jackson, Stephen P

N1 - © 2014 Blasius et al.; Published by Cold Spring Harbor Laboratory Press.

PY - 2014/9/4

Y1 - 2014/9/4

N2 - RNA metabolism is altered following DNA damage, but the underlying mechanisms are not well understood. Through a 14-3-3 interaction screen for DNA damage-induced protein interactions in human cells, we identified protein complexes connected to RNA biology. These include the nuclear exosome targeting (NEXT) complex that regulates turnover of noncoding RNAs termed promoter upstream transcripts (PROMPTs). We show that the NEXT subunit RBM7 is phosphorylated upon DNA damage by the MAPKAPK2 kinase and establish that this mediates 14-3-3 binding and decreases PROMPT binding. These findings and our observation that cells lacking RBM7 display DNA damage hypersensitivity link PROMPT turnover to the DNA damage response.

AB - RNA metabolism is altered following DNA damage, but the underlying mechanisms are not well understood. Through a 14-3-3 interaction screen for DNA damage-induced protein interactions in human cells, we identified protein complexes connected to RNA biology. These include the nuclear exosome targeting (NEXT) complex that regulates turnover of noncoding RNAs termed promoter upstream transcripts (PROMPTs). We show that the NEXT subunit RBM7 is phosphorylated upon DNA damage by the MAPKAPK2 kinase and establish that this mediates 14-3-3 binding and decreases PROMPT binding. These findings and our observation that cells lacking RBM7 display DNA damage hypersensitivity link PROMPT turnover to the DNA damage response.

U2 - 10.1101/gad.246272.114

DO - 10.1101/gad.246272.114

M3 - Journal article

C2 - 25189701

VL - 28

SP - 1977

EP - 1982

JO - Genes & Development

JF - Genes & Development

SN - 0890-9369

IS - 18

ER -

ID: 123734095