A porcine model of subcutaneous Staphylococcus aureus infection: a pilot study

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Standard

A porcine model of subcutaneous Staphylococcus aureus infection : a pilot study. / Nielsen, Ole Lerberg; Mellergaard, Maiken; Frees, Dorte; Larsen, Maria Nygaard; Skov, Søren; Olsen, Lisbeth Høier; Reimann, Maria Josefine.

In: APMIS - Journal of Pathology, Microbiology and Immunology, 2021.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Nielsen, OL, Mellergaard, M, Frees, D, Larsen, MN, Skov, S, Olsen, LH & Reimann, MJ 2021, 'A porcine model of subcutaneous Staphylococcus aureus infection: a pilot study', APMIS - Journal of Pathology, Microbiology and Immunology. https://doi.org/10.1111/apm.13101

APA

Nielsen, O. L., Mellergaard, M., Frees, D., Larsen, M. N., Skov, S., Olsen, L. H., & Reimann, M. J. (2021). A porcine model of subcutaneous Staphylococcus aureus infection: a pilot study. APMIS - Journal of Pathology, Microbiology and Immunology. https://doi.org/10.1111/apm.13101

Vancouver

Nielsen OL, Mellergaard M, Frees D, Larsen MN, Skov S, Olsen LH et al. A porcine model of subcutaneous Staphylococcus aureus infection: a pilot study. APMIS - Journal of Pathology, Microbiology and Immunology. 2021. https://doi.org/10.1111/apm.13101

Author

Nielsen, Ole Lerberg ; Mellergaard, Maiken ; Frees, Dorte ; Larsen, Maria Nygaard ; Skov, Søren ; Olsen, Lisbeth Høier ; Reimann, Maria Josefine. / A porcine model of subcutaneous Staphylococcus aureus infection : a pilot study. In: APMIS - Journal of Pathology, Microbiology and Immunology. 2021.

Bibtex

@article{bade1187df8847859d13a7c25d39fd8b,
title = "A porcine model of subcutaneous Staphylococcus aureus infection: a pilot study",
abstract = "In this descriptive pilot study, we aim to establish a porcine Staphylococcus aureus skin infection model by subcutaneous injection (s.c.) of the porcine S54F9 S. aureus strain in the groin area. Six pigs were used in the study: Five pigs were injected with S. aureus, inocula ranging from 7 × 103 to 5 × 107 colony-forming units per kg bodyweight; one pig was injected with saline exclusively. Lesions were recorded up to 6 days postinoculation using clinical evaluation, ultrasound evaluation, microbiology, flow cytometry, and pathology. Inoculation gave rise to lesions ranging from localized skin infection, that is, minute histological changes, intracellular infection, and macroscopic abscess formation with sequestration of soft tissue, to generalized infection and development of disseminated intravascular coagulation necessitating euthanasia only 10 h after inoculation. Ultrasound assessment of maximum width and characteristics was not able to disclose the progress of the local infection. Flow cytometry and immunohistochemistry revealed the participation of γδT cells in the immune response. In conclusion, we did see a graded inflammatory response associated with the dose of s.c. inoculated bacteria, which may be useful for studying, in particular, the interaction of bacteria and inflammatory mononuclear cell populations. It needs to be investigated if the model is discriminatory and robust.",
keywords = "disseminated intravascular coagulation, intracellular Staphylococcus aureus, pigs, Porcine infection model, subcutaneous abscess",
author = "Nielsen, {Ole Lerberg} and Maiken Mellergaard and Dorte Frees and Larsen, {Maria Nygaard} and S{\o}ren Skov and Olsen, {Lisbeth H{\o}ier} and Reimann, {Maria Josefine}",
year = "2021",
doi = "10.1111/apm.13101",
language = "English",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",

}

RIS

TY - JOUR

T1 - A porcine model of subcutaneous Staphylococcus aureus infection

T2 - a pilot study

AU - Nielsen, Ole Lerberg

AU - Mellergaard, Maiken

AU - Frees, Dorte

AU - Larsen, Maria Nygaard

AU - Skov, Søren

AU - Olsen, Lisbeth Høier

AU - Reimann, Maria Josefine

PY - 2021

Y1 - 2021

N2 - In this descriptive pilot study, we aim to establish a porcine Staphylococcus aureus skin infection model by subcutaneous injection (s.c.) of the porcine S54F9 S. aureus strain in the groin area. Six pigs were used in the study: Five pigs were injected with S. aureus, inocula ranging from 7 × 103 to 5 × 107 colony-forming units per kg bodyweight; one pig was injected with saline exclusively. Lesions were recorded up to 6 days postinoculation using clinical evaluation, ultrasound evaluation, microbiology, flow cytometry, and pathology. Inoculation gave rise to lesions ranging from localized skin infection, that is, minute histological changes, intracellular infection, and macroscopic abscess formation with sequestration of soft tissue, to generalized infection and development of disseminated intravascular coagulation necessitating euthanasia only 10 h after inoculation. Ultrasound assessment of maximum width and characteristics was not able to disclose the progress of the local infection. Flow cytometry and immunohistochemistry revealed the participation of γδT cells in the immune response. In conclusion, we did see a graded inflammatory response associated with the dose of s.c. inoculated bacteria, which may be useful for studying, in particular, the interaction of bacteria and inflammatory mononuclear cell populations. It needs to be investigated if the model is discriminatory and robust.

AB - In this descriptive pilot study, we aim to establish a porcine Staphylococcus aureus skin infection model by subcutaneous injection (s.c.) of the porcine S54F9 S. aureus strain in the groin area. Six pigs were used in the study: Five pigs were injected with S. aureus, inocula ranging from 7 × 103 to 5 × 107 colony-forming units per kg bodyweight; one pig was injected with saline exclusively. Lesions were recorded up to 6 days postinoculation using clinical evaluation, ultrasound evaluation, microbiology, flow cytometry, and pathology. Inoculation gave rise to lesions ranging from localized skin infection, that is, minute histological changes, intracellular infection, and macroscopic abscess formation with sequestration of soft tissue, to generalized infection and development of disseminated intravascular coagulation necessitating euthanasia only 10 h after inoculation. Ultrasound assessment of maximum width and characteristics was not able to disclose the progress of the local infection. Flow cytometry and immunohistochemistry revealed the participation of γδT cells in the immune response. In conclusion, we did see a graded inflammatory response associated with the dose of s.c. inoculated bacteria, which may be useful for studying, in particular, the interaction of bacteria and inflammatory mononuclear cell populations. It needs to be investigated if the model is discriminatory and robust.

KW - disseminated intravascular coagulation

KW - intracellular Staphylococcus aureus

KW - pigs

KW - Porcine infection model

KW - subcutaneous abscess

U2 - 10.1111/apm.13101

DO - 10.1111/apm.13101

M3 - Journal article

C2 - 33644910

AN - SCOPUS:85101930756

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

ER -

ID: 259000509