A novel marker for assessment of liver matrix remodeling: An enzyme-linked immunosorbent assay (ELISA) detecting a MMP generated type I collagen neo-epitope (C1M)

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A novel marker for assessment of liver matrix remodeling : An enzyme-linked immunosorbent assay (ELISA) detecting a MMP generated type I collagen neo-epitope (C1M). / Leeming, D. J.; He, Y.; Veidal, S. S.; Nguyen, QHT; Larsen, D. V.; Koizumi, M.; Segovia-Silvestre, T.; Zhang, C.; Zheng, Q.; Sun, S.; Cao, Y.; Barkholt, V.; Hägglund, P.; Bay-Jensen, A. C.; Qvist, P.; Karsdal, M. A.

In: Biomarkers, Vol. 16, No. 7, 01.11.2011, p. 616-628.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Leeming, DJ, He, Y, Veidal, SS, Nguyen, QHT, Larsen, DV, Koizumi, M, Segovia-Silvestre, T, Zhang, C, Zheng, Q, Sun, S, Cao, Y, Barkholt, V, Hägglund, P, Bay-Jensen, AC, Qvist, P & Karsdal, MA 2011, 'A novel marker for assessment of liver matrix remodeling: An enzyme-linked immunosorbent assay (ELISA) detecting a MMP generated type I collagen neo-epitope (C1M)', Biomarkers, vol. 16, no. 7, pp. 616-628. https://doi.org/10.3109/1354750X.2011.620628

APA

Leeming, D. J., He, Y., Veidal, S. S., Nguyen, QHT., Larsen, D. V., Koizumi, M., Segovia-Silvestre, T., Zhang, C., Zheng, Q., Sun, S., Cao, Y., Barkholt, V., Hägglund, P., Bay-Jensen, A. C., Qvist, P., & Karsdal, M. A. (2011). A novel marker for assessment of liver matrix remodeling: An enzyme-linked immunosorbent assay (ELISA) detecting a MMP generated type I collagen neo-epitope (C1M). Biomarkers, 16(7), 616-628. https://doi.org/10.3109/1354750X.2011.620628

Vancouver

Leeming DJ, He Y, Veidal SS, Nguyen QHT, Larsen DV, Koizumi M et al. A novel marker for assessment of liver matrix remodeling: An enzyme-linked immunosorbent assay (ELISA) detecting a MMP generated type I collagen neo-epitope (C1M). Biomarkers. 2011 Nov 1;16(7):616-628. https://doi.org/10.3109/1354750X.2011.620628

Author

Leeming, D. J. ; He, Y. ; Veidal, S. S. ; Nguyen, QHT ; Larsen, D. V. ; Koizumi, M. ; Segovia-Silvestre, T. ; Zhang, C. ; Zheng, Q. ; Sun, S. ; Cao, Y. ; Barkholt, V. ; Hägglund, P. ; Bay-Jensen, A. C. ; Qvist, P. ; Karsdal, M. A. / A novel marker for assessment of liver matrix remodeling : An enzyme-linked immunosorbent assay (ELISA) detecting a MMP generated type I collagen neo-epitope (C1M). In: Biomarkers. 2011 ; Vol. 16, No. 7. pp. 616-628.

Bibtex

@article{77e9e5569f0640a49cdd89198d325d3a,
title = "A novel marker for assessment of liver matrix remodeling: An enzyme-linked immunosorbent assay (ELISA) detecting a MMP generated type I collagen neo-epitope (C1M)",
abstract = "A competitive enzyme-linked immunosorbent assay (ELISA) for detection of a type I collagen fragment generated by matrix metalloproteinases (MMP) -2, -9 and -13, was developed (CO1-764 or C1M). The biomarker was evaluated in two preclinical rat models of liver fibrosis: bile duct ligation (BDL) and carbon tetra chloride (CCL4)-treated rats. The assay was further evaluated in a clinical study of prostate-, lung- and breast-cancer patients stratified according to skeletal metastases. A technically robust ELISA assay specific for a MMP-2, -9 and -13 neo-epitope was produced and seen to be statistically elevated in BDL rats compared to baseline levels as well as significantly elevated in CCL4 rats stratified according to the amount of total collagen in the livers. CO1-764 levels also correlated significantly with total liver collagen and type I collagen mRNA expression in the livers. Finally, the CO1-764 marker was not correlated with skeletal involvement or number of bone metastases. This ELISA has the potential to assess the degree of liver fibrosis in a non-invasive manner.",
keywords = "bile duct ligation, Biochemical markers, bone metastases, breast cancer, CCL4, liver fibrosis, MMP-2,-9,-13, prostate cancer, protease-cleaved neo-epitope, rat model, translational science, type I collagen",
author = "Leeming, {D. J.} and Y. He and Veidal, {S. S.} and QHT Nguyen and Larsen, {D. V.} and M. Koizumi and T. Segovia-Silvestre and C. Zhang and Q. Zheng and S. Sun and Y. Cao and V. Barkholt and P. H{\"a}gglund and Bay-Jensen, {A. C.} and P. Qvist and Karsdal, {M. A.}",
year = "2011",
month = nov,
day = "1",
doi = "10.3109/1354750X.2011.620628",
language = "English",
volume = "16",
pages = "616--628",
journal = "Biomarkers",
issn = "1354-750X",
publisher = "Taylor & Francis",
number = "7",

}

RIS

TY - JOUR

T1 - A novel marker for assessment of liver matrix remodeling

T2 - An enzyme-linked immunosorbent assay (ELISA) detecting a MMP generated type I collagen neo-epitope (C1M)

AU - Leeming, D. J.

AU - He, Y.

AU - Veidal, S. S.

AU - Nguyen, QHT

AU - Larsen, D. V.

AU - Koizumi, M.

AU - Segovia-Silvestre, T.

AU - Zhang, C.

AU - Zheng, Q.

AU - Sun, S.

AU - Cao, Y.

AU - Barkholt, V.

AU - Hägglund, P.

AU - Bay-Jensen, A. C.

AU - Qvist, P.

AU - Karsdal, M. A.

PY - 2011/11/1

Y1 - 2011/11/1

N2 - A competitive enzyme-linked immunosorbent assay (ELISA) for detection of a type I collagen fragment generated by matrix metalloproteinases (MMP) -2, -9 and -13, was developed (CO1-764 or C1M). The biomarker was evaluated in two preclinical rat models of liver fibrosis: bile duct ligation (BDL) and carbon tetra chloride (CCL4)-treated rats. The assay was further evaluated in a clinical study of prostate-, lung- and breast-cancer patients stratified according to skeletal metastases. A technically robust ELISA assay specific for a MMP-2, -9 and -13 neo-epitope was produced and seen to be statistically elevated in BDL rats compared to baseline levels as well as significantly elevated in CCL4 rats stratified according to the amount of total collagen in the livers. CO1-764 levels also correlated significantly with total liver collagen and type I collagen mRNA expression in the livers. Finally, the CO1-764 marker was not correlated with skeletal involvement or number of bone metastases. This ELISA has the potential to assess the degree of liver fibrosis in a non-invasive manner.

AB - A competitive enzyme-linked immunosorbent assay (ELISA) for detection of a type I collagen fragment generated by matrix metalloproteinases (MMP) -2, -9 and -13, was developed (CO1-764 or C1M). The biomarker was evaluated in two preclinical rat models of liver fibrosis: bile duct ligation (BDL) and carbon tetra chloride (CCL4)-treated rats. The assay was further evaluated in a clinical study of prostate-, lung- and breast-cancer patients stratified according to skeletal metastases. A technically robust ELISA assay specific for a MMP-2, -9 and -13 neo-epitope was produced and seen to be statistically elevated in BDL rats compared to baseline levels as well as significantly elevated in CCL4 rats stratified according to the amount of total collagen in the livers. CO1-764 levels also correlated significantly with total liver collagen and type I collagen mRNA expression in the livers. Finally, the CO1-764 marker was not correlated with skeletal involvement or number of bone metastases. This ELISA has the potential to assess the degree of liver fibrosis in a non-invasive manner.

KW - bile duct ligation

KW - Biochemical markers

KW - bone metastases

KW - breast cancer

KW - CCL4

KW - liver fibrosis

KW - MMP-2,-9,-13

KW - prostate cancer

KW - protease-cleaved neo-epitope

KW - rat model

KW - translational science

KW - type I collagen

U2 - 10.3109/1354750X.2011.620628

DO - 10.3109/1354750X.2011.620628

M3 - Journal article

C2 - 21988680

AN - SCOPUS:80054906939

VL - 16

SP - 616

EP - 628

JO - Biomarkers

JF - Biomarkers

SN - 1354-750X

IS - 7

ER -

ID: 240160089