TY - JOUR

T1 - A metabolite roadmap of the wood-forming tissue in Populus tremula

AU - Abreu, Ilka N.

AU - Johansson, Annika I.

AU - Sokolowska, Katarzyna

AU - Niittyla, Totte

AU - Sundberg, Bjorn

AU - Hvidsten, Torgeir R.

AU - Street, Nathaniel R.

AU - Moritz, Thomas

PY - 2020

Y1 - 2020

N2 - Wood, or secondary xylem, is the product of xylogenesis, a developmental process that begins with the proliferation of cambial derivatives and ends with mature xylem fibers and vessels with lignified secondary cell walls. Fully mature xylem has undergone a series of cellular processes, including cell division, cell expansion, secondary wall formation, lignification and programmed cell death. A complex network of interactions between transcriptional regulators and signal transduction pathways controls wood formation. However, the role of metabolites during this developmental process has not been comprehensively characterized. To evaluate the role of metabolites during wood formation, we performed a high spatial resolution metabolomics study of the wood-forming zone ofPopulus tremula,including laser dissected aspen ray and fiber cells. We show that metabolites show specific patterns within the wood-forming zone, following the differentiation process from cell division to cell death. The data from profiled laser dissected aspen ray and fiber cells suggests that these two cell types host distinctly different metabolic processes. Furthermore, by integrating previously published transcriptomic and proteomic profiles generated from the same trees, we provide an integrative picture of molecular processes, for example, deamination of phenylalanine during lignification is of critical importance for nitrogen metabolism during wood formation.

AB - Wood, or secondary xylem, is the product of xylogenesis, a developmental process that begins with the proliferation of cambial derivatives and ends with mature xylem fibers and vessels with lignified secondary cell walls. Fully mature xylem has undergone a series of cellular processes, including cell division, cell expansion, secondary wall formation, lignification and programmed cell death. A complex network of interactions between transcriptional regulators and signal transduction pathways controls wood formation. However, the role of metabolites during this developmental process has not been comprehensively characterized. To evaluate the role of metabolites during wood formation, we performed a high spatial resolution metabolomics study of the wood-forming zone ofPopulus tremula,including laser dissected aspen ray and fiber cells. We show that metabolites show specific patterns within the wood-forming zone, following the differentiation process from cell division to cell death. The data from profiled laser dissected aspen ray and fiber cells suggests that these two cell types host distinctly different metabolic processes. Furthermore, by integrating previously published transcriptomic and proteomic profiles generated from the same trees, we provide an integrative picture of molecular processes, for example, deamination of phenylalanine during lignification is of critical importance for nitrogen metabolism during wood formation.

KW - cambium

KW - crysectioning

KW - laser capture microdissection

KW - metabolomics

KW - Populus

KW - wood

KW - PHENOLIC GLYCOSIDES

KW - WALL FORMATION

KW - CELL-DEATH

KW - POPULUS

KW - BIOSYNTHESIS

KW - AUXIN

KW - GIBBERELLIN

KW - SECONDARY

KW - GROWTH

KW - LIGNIN

U2 - 10.1111/nph.16799

DO - 10.1111/nph.16799

M3 - Journal article

C2 - 32648607

VL - 228

SP - 1559

EP - 1572

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

ER -