A metabolite roadmap of the wood-forming tissue in Populus tremula
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A metabolite roadmap of the wood-forming tissue in Populus tremula. / Abreu, Ilka N.; Johansson, Annika I.; Sokolowska, Katarzyna; Niittyla, Totte; Sundberg, Bjorn; Hvidsten, Torgeir R.; Street, Nathaniel R.; Moritz, Thomas.
In: New Phytologist, Vol. 228, 2020, p. 1559-1572.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A metabolite roadmap of the wood-forming tissue in Populus tremula
AU - Abreu, Ilka N.
AU - Johansson, Annika I.
AU - Sokolowska, Katarzyna
AU - Niittyla, Totte
AU - Sundberg, Bjorn
AU - Hvidsten, Torgeir R.
AU - Street, Nathaniel R.
AU - Moritz, Thomas
PY - 2020
Y1 - 2020
N2 - Wood, or secondary xylem, is the product of xylogenesis, a developmental process that begins with the proliferation of cambial derivatives and ends with mature xylem fibers and vessels with lignified secondary cell walls. Fully mature xylem has undergone a series of cellular processes, including cell division, cell expansion, secondary wall formation, lignification and programmed cell death. A complex network of interactions between transcriptional regulators and signal transduction pathways controls wood formation. However, the role of metabolites during this developmental process has not been comprehensively characterized. To evaluate the role of metabolites during wood formation, we performed a high spatial resolution metabolomics study of the wood-forming zone ofPopulus tremula,including laser dissected aspen ray and fiber cells. We show that metabolites show specific patterns within the wood-forming zone, following the differentiation process from cell division to cell death. The data from profiled laser dissected aspen ray and fiber cells suggests that these two cell types host distinctly different metabolic processes. Furthermore, by integrating previously published transcriptomic and proteomic profiles generated from the same trees, we provide an integrative picture of molecular processes, for example, deamination of phenylalanine during lignification is of critical importance for nitrogen metabolism during wood formation.
AB - Wood, or secondary xylem, is the product of xylogenesis, a developmental process that begins with the proliferation of cambial derivatives and ends with mature xylem fibers and vessels with lignified secondary cell walls. Fully mature xylem has undergone a series of cellular processes, including cell division, cell expansion, secondary wall formation, lignification and programmed cell death. A complex network of interactions between transcriptional regulators and signal transduction pathways controls wood formation. However, the role of metabolites during this developmental process has not been comprehensively characterized. To evaluate the role of metabolites during wood formation, we performed a high spatial resolution metabolomics study of the wood-forming zone ofPopulus tremula,including laser dissected aspen ray and fiber cells. We show that metabolites show specific patterns within the wood-forming zone, following the differentiation process from cell division to cell death. The data from profiled laser dissected aspen ray and fiber cells suggests that these two cell types host distinctly different metabolic processes. Furthermore, by integrating previously published transcriptomic and proteomic profiles generated from the same trees, we provide an integrative picture of molecular processes, for example, deamination of phenylalanine during lignification is of critical importance for nitrogen metabolism during wood formation.
KW - cambium
KW - crysectioning
KW - laser capture microdissection
KW - metabolomics
KW - Populus
KW - wood
KW - PHENOLIC GLYCOSIDES
KW - WALL FORMATION
KW - CELL-DEATH
KW - POPULUS
KW - BIOSYNTHESIS
KW - AUXIN
KW - GIBBERELLIN
KW - SECONDARY
KW - GROWTH
KW - LIGNIN
U2 - 10.1111/nph.16799
DO - 10.1111/nph.16799
M3 - Journal article
C2 - 32648607
VL - 228
SP - 1559
EP - 1572
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
ER -
ID: 250118479