Proteomic changes in Debaryomyces hansenii upon exposure to NaCl stress

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Proteomic changes in Debaryomyces hansenii upon exposure to NaCl stress. / Gori, Klaus; Hébraud, Michel; Chambon, Christophe; Mortensen, Henrik Dam; Arneborg, Nils; Jespersen, Lene.

In: FEMS Yeast Research, Vol. 7, No. 2, 2007, p. 293-303.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Gori, K, Hébraud, M, Chambon, C, Mortensen, HD, Arneborg, N & Jespersen, L 2007, 'Proteomic changes in Debaryomyces hansenii upon exposure to NaCl stress', FEMS Yeast Research, vol. 7, no. 2, pp. 293-303. https://doi.org/10.1111/j.1567-1364.2006.00155.x

APA

Gori, K., Hébraud, M., Chambon, C., Mortensen, H. D., Arneborg, N., & Jespersen, L. (2007). Proteomic changes in Debaryomyces hansenii upon exposure to NaCl stress. FEMS Yeast Research, 7(2), 293-303. https://doi.org/10.1111/j.1567-1364.2006.00155.x

Vancouver

Gori K, Hébraud M, Chambon C, Mortensen HD, Arneborg N, Jespersen L. Proteomic changes in Debaryomyces hansenii upon exposure to NaCl stress. FEMS Yeast Research. 2007;7(2):293-303. https://doi.org/10.1111/j.1567-1364.2006.00155.x

Author

Gori, Klaus ; Hébraud, Michel ; Chambon, Christophe ; Mortensen, Henrik Dam ; Arneborg, Nils ; Jespersen, Lene. / Proteomic changes in Debaryomyces hansenii upon exposure to NaCl stress. In: FEMS Yeast Research. 2007 ; Vol. 7, No. 2. pp. 293-303.

Bibtex

@article{0d7f6e60a1c411ddb6ae000ea68e967b,
title = "Proteomic changes in Debaryomyces hansenii upon exposure to NaCl stress",
abstract = "The proteome of the highly NaCl-tolerant yeast Debaryomyces hansenii was investigated by two-dimensional polyacrylamide gel electrophoresis (2D PAGE), and 47 protein spots were identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) followed by mass spectrometry (MS). The influence of NaCl on the D. hansenii proteome was investigated during the first 3 h of NaCl exposure. The rate of protein synthesis was strongly decreased by exposure to 8{\%} and 12{\%} (w/v) NaCl, as the average incorporation rates of l-[(35)S]methionine within the first 30 min after addition of NaCl were only 7{\%} and 4{\%} of the rate in medium without NaCl. In addition, the number of protein spots detected on 2D gels prepared from cells exposed to 8{\%} and 12{\%} (w/v) NaCl exceeded less than 28{\%} of the number of protein spots detected on 2D gels prepared from cells without added NaCl. Several proteins were identified as being either induced or repressed upon NaCl exposure. The induced proteins were enzymes involved in glycerol synthesis/dissimilation and the upper part of glycolysis, whereas the repressed proteins were enzymes involved in the lower part of glycolysis, the route to the Krebs cycle, and the synthesis of amino acids. Furthermore, one heat shock protein (Ssa1p) was induced, whereas others (Ssb2p and Hsp60p) were repressed.",
keywords = "Former LIFE faculty, Debaryomyces hansenii; proteome; 2D PAGE; NaCl stress",
author = "Klaus Gori and Michel H{\'e}braud and Christophe Chambon and Mortensen, {Henrik Dam} and Nils Arneborg and Lene Jespersen",
year = "2007",
doi = "10.1111/j.1567-1364.2006.00155.x",
language = "English",
volume = "7",
pages = "293--303",
journal = "F E M S Yeast Research",
issn = "1567-1356",
publisher = "Oxford University Press",
number = "2",

}

RIS

TY - JOUR

T1 - Proteomic changes in Debaryomyces hansenii upon exposure to NaCl stress

AU - Gori, Klaus

AU - Hébraud, Michel

AU - Chambon, Christophe

AU - Mortensen, Henrik Dam

AU - Arneborg, Nils

AU - Jespersen, Lene

PY - 2007

Y1 - 2007

N2 - The proteome of the highly NaCl-tolerant yeast Debaryomyces hansenii was investigated by two-dimensional polyacrylamide gel electrophoresis (2D PAGE), and 47 protein spots were identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) followed by mass spectrometry (MS). The influence of NaCl on the D. hansenii proteome was investigated during the first 3 h of NaCl exposure. The rate of protein synthesis was strongly decreased by exposure to 8% and 12% (w/v) NaCl, as the average incorporation rates of l-[(35)S]methionine within the first 30 min after addition of NaCl were only 7% and 4% of the rate in medium without NaCl. In addition, the number of protein spots detected on 2D gels prepared from cells exposed to 8% and 12% (w/v) NaCl exceeded less than 28% of the number of protein spots detected on 2D gels prepared from cells without added NaCl. Several proteins were identified as being either induced or repressed upon NaCl exposure. The induced proteins were enzymes involved in glycerol synthesis/dissimilation and the upper part of glycolysis, whereas the repressed proteins were enzymes involved in the lower part of glycolysis, the route to the Krebs cycle, and the synthesis of amino acids. Furthermore, one heat shock protein (Ssa1p) was induced, whereas others (Ssb2p and Hsp60p) were repressed.

AB - The proteome of the highly NaCl-tolerant yeast Debaryomyces hansenii was investigated by two-dimensional polyacrylamide gel electrophoresis (2D PAGE), and 47 protein spots were identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) followed by mass spectrometry (MS). The influence of NaCl on the D. hansenii proteome was investigated during the first 3 h of NaCl exposure. The rate of protein synthesis was strongly decreased by exposure to 8% and 12% (w/v) NaCl, as the average incorporation rates of l-[(35)S]methionine within the first 30 min after addition of NaCl were only 7% and 4% of the rate in medium without NaCl. In addition, the number of protein spots detected on 2D gels prepared from cells exposed to 8% and 12% (w/v) NaCl exceeded less than 28% of the number of protein spots detected on 2D gels prepared from cells without added NaCl. Several proteins were identified as being either induced or repressed upon NaCl exposure. The induced proteins were enzymes involved in glycerol synthesis/dissimilation and the upper part of glycolysis, whereas the repressed proteins were enzymes involved in the lower part of glycolysis, the route to the Krebs cycle, and the synthesis of amino acids. Furthermore, one heat shock protein (Ssa1p) was induced, whereas others (Ssb2p and Hsp60p) were repressed.

KW - Former LIFE faculty

KW - Debaryomyces hansenii; proteome; 2D PAGE; NaCl stress

U2 - 10.1111/j.1567-1364.2006.00155.x

DO - 10.1111/j.1567-1364.2006.00155.x

M3 - Journal article

VL - 7

SP - 293

EP - 303

JO - F E M S Yeast Research

JF - F E M S Yeast Research

SN - 1567-1356

IS - 2

ER -

ID: 8108479