The forebrain synaptic transcriptome is organized by clocks but its proteome is driven by sleep

Research output: Contribution to journalJournal articleResearchpeer-review

Sara B Noya, David Colameo, Franziska Brüning, Andrea Spinnler, Dennis Mircsof, Lennart Opitz, Matthias Mann, Shiva K Tyagarajan, Maria S Robles, Steven A Brown

Neurons have adapted mechanisms to traffic RNA and protein into distant dendritic and axonal arbors. Taking a biochemical approach, we reveal that forebrain synaptic transcript accumulation shows overwhelmingly daily rhythms, with two-thirds of synaptic transcripts showing time-of-day-dependent abundance independent of oscillations in the soma. These transcripts formed two sharp temporal and functional clusters, with transcripts preceding dawn related to metabolism and translation and those anticipating dusk related to synaptic transmission. Characterization of the synaptic proteome around the clock demonstrates the functional relevance of temporal gating for synaptic processes and energy homeostasis. Unexpectedly, sleep deprivation completely abolished proteome but not transcript oscillations. Altogether, the emerging picture is one of a circadian anticipation of messenger RNA needs in the synapse followed by translation as demanded by sleep-wake cycles.

Original languageEnglish
Article numbereaav2642
JournalScience (New York, N.Y.)
Volume366
Issue number6462
ISSN0036-8075
DOIs
Publication statusPublished - 11 Oct 2019
Externally publishedYes

Bibliographical note

Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

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