Pancreatic beta-cell responses to GLP-1 after near-normalization of blood glucose in patients with type 2 diabetes

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Pancreatic beta-cell responses to GLP-1 after near-normalization of blood glucose in patients with type 2 diabetes. / Asmar, Meena; Højberg, Patricia V; Deacon, Carolyn F; Hare, Kristine; Holst, Jens Juul; Madsbad, Sten.

In: Regulatory Peptides, Vol. 160, No. 1-3, 2010, p. 175-80.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Asmar, M, Højberg, PV, Deacon, CF, Hare, K, Holst, JJ & Madsbad, S 2010, 'Pancreatic beta-cell responses to GLP-1 after near-normalization of blood glucose in patients with type 2 diabetes', Regulatory Peptides, vol. 160, no. 1-3, pp. 175-80. https://doi.org/10.1016/j.regpep.2009.12.004

APA

Asmar, M., Højberg, P. V., Deacon, C. F., Hare, K., Holst, J. J., & Madsbad, S. (2010). Pancreatic beta-cell responses to GLP-1 after near-normalization of blood glucose in patients with type 2 diabetes. Regulatory Peptides, 160(1-3), 175-80. https://doi.org/10.1016/j.regpep.2009.12.004

Vancouver

Asmar M, Højberg PV, Deacon CF, Hare K, Holst JJ, Madsbad S. Pancreatic beta-cell responses to GLP-1 after near-normalization of blood glucose in patients with type 2 diabetes. Regulatory Peptides. 2010;160(1-3):175-80. https://doi.org/10.1016/j.regpep.2009.12.004

Author

Asmar, Meena ; Højberg, Patricia V ; Deacon, Carolyn F ; Hare, Kristine ; Holst, Jens Juul ; Madsbad, Sten. / Pancreatic beta-cell responses to GLP-1 after near-normalization of blood glucose in patients with type 2 diabetes. In: Regulatory Peptides. 2010 ; Vol. 160, No. 1-3. pp. 175-80.

Bibtex

@article{a6d315370a9b4d26b70441b5d6be8f5a,
title = "Pancreatic beta-cell responses to GLP-1 after near-normalization of blood glucose in patients with type 2 diabetes",
abstract = "This study investigated the effects of strict glycaemic control on beta-cell function in nine obese subjects with type 2 diabetes (T2DM), using graded glucose infusions together with infusions of saline or GLP-1 before (HbA(1)c: 8.0+/-0.4{\%}) and after four weeks of near-normalization of blood glucose (BG) using insulin (mean diurnal BG: 6.4+/-0.3 mmol/l; HbA(1)c: 6.6+/-0.3{\%}). Nine matched healthy subjects acted as controls. In controls, area-under-curve (AUC) for amylin, C-peptide and proinsulin were higher with GLP-1 than saline (P<0.001). The AUC amylin/C-peptide ratio was similar on both days, while AUC proinsulin/C-peptide ratio was higher with GLP-1 (P=0.02). In the patients, amylin, C-peptide and proinsulin AUCs were unaltered by near-normoglycaemia per se. Proinsulin responses to GLP-1 were unchanged, but amylin and C-peptide AUCs increased (P<0.05) after insulin treatment, and AUC amylin/C-peptide ratios rose to control levels. Near-normoglycaemia tended to reduce AUC proinsulin/C-peptide ratio, which was significant (P=0.04) with GLP-1, but still higher than with saline (P=0.004). In conclusion, amylin, C-peptide and proinsulin responses to glucose were unaffected by four weeks of near-normoglycaemia, whereas GLP-1 increased amylin and C-peptide secretion and amylin/C-peptide ratio. Near-normoglycaemia reduced proinsulin/C-peptide ratio during stimulation with GLP-1, suggesting that strict glycaemic control might ameliorate some of the disturbances in beta-cell function characterizing T2DM.",
keywords = "Amyloid, Area Under Curve, Blood Glucose, C-Peptide, Diabetes Mellitus, Type 2, Female, Glucagon-Like Peptide 1, Glucose, Humans, Incretins, Insulin, Insulin-Secreting Cells, Islet Amyloid Polypeptide, Male, Middle Aged, Proinsulin, Time Factors",
author = "Meena Asmar and H{\o}jberg, {Patricia V} and Deacon, {Carolyn F} and Kristine Hare and Holst, {Jens Juul} and Sten Madsbad",
note = "Copyright 2010 Elsevier B.V. All rights reserved.",
year = "2010",
doi = "10.1016/j.regpep.2009.12.004",
language = "English",
volume = "160",
pages = "175--80",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "1-3",

}

RIS

TY - JOUR

T1 - Pancreatic beta-cell responses to GLP-1 after near-normalization of blood glucose in patients with type 2 diabetes

AU - Asmar, Meena

AU - Højberg, Patricia V

AU - Deacon, Carolyn F

AU - Hare, Kristine

AU - Holst, Jens Juul

AU - Madsbad, Sten

N1 - Copyright 2010 Elsevier B.V. All rights reserved.

PY - 2010

Y1 - 2010

N2 - This study investigated the effects of strict glycaemic control on beta-cell function in nine obese subjects with type 2 diabetes (T2DM), using graded glucose infusions together with infusions of saline or GLP-1 before (HbA(1)c: 8.0+/-0.4%) and after four weeks of near-normalization of blood glucose (BG) using insulin (mean diurnal BG: 6.4+/-0.3 mmol/l; HbA(1)c: 6.6+/-0.3%). Nine matched healthy subjects acted as controls. In controls, area-under-curve (AUC) for amylin, C-peptide and proinsulin were higher with GLP-1 than saline (P<0.001). The AUC amylin/C-peptide ratio was similar on both days, while AUC proinsulin/C-peptide ratio was higher with GLP-1 (P=0.02). In the patients, amylin, C-peptide and proinsulin AUCs were unaltered by near-normoglycaemia per se. Proinsulin responses to GLP-1 were unchanged, but amylin and C-peptide AUCs increased (P<0.05) after insulin treatment, and AUC amylin/C-peptide ratios rose to control levels. Near-normoglycaemia tended to reduce AUC proinsulin/C-peptide ratio, which was significant (P=0.04) with GLP-1, but still higher than with saline (P=0.004). In conclusion, amylin, C-peptide and proinsulin responses to glucose were unaffected by four weeks of near-normoglycaemia, whereas GLP-1 increased amylin and C-peptide secretion and amylin/C-peptide ratio. Near-normoglycaemia reduced proinsulin/C-peptide ratio during stimulation with GLP-1, suggesting that strict glycaemic control might ameliorate some of the disturbances in beta-cell function characterizing T2DM.

AB - This study investigated the effects of strict glycaemic control on beta-cell function in nine obese subjects with type 2 diabetes (T2DM), using graded glucose infusions together with infusions of saline or GLP-1 before (HbA(1)c: 8.0+/-0.4%) and after four weeks of near-normalization of blood glucose (BG) using insulin (mean diurnal BG: 6.4+/-0.3 mmol/l; HbA(1)c: 6.6+/-0.3%). Nine matched healthy subjects acted as controls. In controls, area-under-curve (AUC) for amylin, C-peptide and proinsulin were higher with GLP-1 than saline (P<0.001). The AUC amylin/C-peptide ratio was similar on both days, while AUC proinsulin/C-peptide ratio was higher with GLP-1 (P=0.02). In the patients, amylin, C-peptide and proinsulin AUCs were unaltered by near-normoglycaemia per se. Proinsulin responses to GLP-1 were unchanged, but amylin and C-peptide AUCs increased (P<0.05) after insulin treatment, and AUC amylin/C-peptide ratios rose to control levels. Near-normoglycaemia tended to reduce AUC proinsulin/C-peptide ratio, which was significant (P=0.04) with GLP-1, but still higher than with saline (P=0.004). In conclusion, amylin, C-peptide and proinsulin responses to glucose were unaffected by four weeks of near-normoglycaemia, whereas GLP-1 increased amylin and C-peptide secretion and amylin/C-peptide ratio. Near-normoglycaemia reduced proinsulin/C-peptide ratio during stimulation with GLP-1, suggesting that strict glycaemic control might ameliorate some of the disturbances in beta-cell function characterizing T2DM.

KW - Amyloid

KW - Area Under Curve

KW - Blood Glucose

KW - C-Peptide

KW - Diabetes Mellitus, Type 2

KW - Female

KW - Glucagon-Like Peptide 1

KW - Glucose

KW - Humans

KW - Incretins

KW - Insulin

KW - Insulin-Secreting Cells

KW - Islet Amyloid Polypeptide

KW - Male

KW - Middle Aged

KW - Proinsulin

KW - Time Factors

U2 - 10.1016/j.regpep.2009.12.004

DO - 10.1016/j.regpep.2009.12.004

M3 - Journal article

VL - 160

SP - 175

EP - 180

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 1-3

ER -

ID: 33940451