Once in a lifetime: strategies for preventing re-replication in prokaryotic and eukaryotic cells.

Research output: Contribution to journalJournal articleResearchpeer-review

Documents

  • Embor20082

    Submitted manuscript, 249 KB, PDF-document

Olaf Nielsen, Anders Løbner-Olesen

DNA replication is an extremely accurate process and cells have evolved intricate control mechanisms to ensure that each region of their genome is replicated only once during S phase. Here, we compare what is known about the processes that prevent re-replication in prokaryotic and eukaryotic cells by using the model organisms Escherichia coli and Schizosaccharomyces pombe as examples. Although the underlying molecular details are different, the logic behind the control mechanisms is similar. For example, after initiation, crucial molecules required for the loading of replicative helicases in both prokaryotes and eukaryotes are inactivated until the next cell cycle. Furthermore, in both systems the beta-clamp of the replicative polymerase associates with enzymatic activities that contribute to the inactivation of the helicase loaders. Finally, recent studies suggest that the control mechanism that prevents re-replication in both systems also increases the synthesis of DNA building blocks.
Original languageEnglish
JournalEMBO Reports
Volume9
Issue number2
Pages (from-to)151-6
Number of pages5
ISSN1469-221X
DOIs
Publication statusPublished - 2008

Bibliographical note

Keywords: DNA Replication; Escherichia coli; Eukaryotic Cells; Nucleotides; Prokaryotic Cells; Schizosaccharomyces

Number of downloads are based on statistics from Google Scholar and www.ku.dk


No data available

ID: 5626518