High-throughput verification of transcriptional starting sites by Deep-RACE

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Signe Olivarius, Charles Plessy, Piero Carninci

We present a high-throughput method for investigating the transcriptional starting sites of genes of interest, which we named Deep-RACE (Deep–rapid amplification of cDNA ends). Taking advantage of the latest sequencing technology, it allows the parallel analysis of multiple genes and is free of time-consuming cloning steps. In comparison to the sequencing of RACE PCR products, our approach is more precise and more cost-effective even for batches as small as 17.
Original languageEnglish
JournalBioTechniques
Volume46
Issue number2
Pages (from-to)130-132
Number of pages3
ISSN0736-6205
DOIs
Publication statusPublished - 2009

    Research areas

  • The Faculty of Science - promoters, transcription start sites, high throughput, RACE, short-reads sequencing

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