Genotoxic potential of the perfluorinated chemicals PFOA, PFOS, PFBS, PFNA and PFHxA in human HepG2 cells

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Genotoxic potential of the perfluorinated chemicals PFOA, PFOS, PFBS, PFNA and PFHxA in human HepG2 cells. / Eriksen, Kirsten Thorup; Raaschou-Nielsen, Ole; Sørensen, Mette; Roursgaard, Martin; Loft, Steffen; Møller, Peter.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 700, No. 1-2, 2010, p. 39-43.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Eriksen, KT, Raaschou-Nielsen, O, Sørensen, M, Roursgaard, M, Loft, S & Møller, P 2010, 'Genotoxic potential of the perfluorinated chemicals PFOA, PFOS, PFBS, PFNA and PFHxA in human HepG2 cells', Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, vol. 700, no. 1-2, pp. 39-43. https://doi.org/10.1016/j.mrgentox.2010.04.024

APA

Eriksen, K. T., Raaschou-Nielsen, O., Sørensen, M., Roursgaard, M., Loft, S., & Møller, P. (2010). Genotoxic potential of the perfluorinated chemicals PFOA, PFOS, PFBS, PFNA and PFHxA in human HepG2 cells. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 700(1-2), 39-43. https://doi.org/10.1016/j.mrgentox.2010.04.024

Vancouver

Eriksen KT, Raaschou-Nielsen O, Sørensen M, Roursgaard M, Loft S, Møller P. Genotoxic potential of the perfluorinated chemicals PFOA, PFOS, PFBS, PFNA and PFHxA in human HepG2 cells. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2010;700(1-2):39-43. https://doi.org/10.1016/j.mrgentox.2010.04.024

Author

Eriksen, Kirsten Thorup ; Raaschou-Nielsen, Ole ; Sørensen, Mette ; Roursgaard, Martin ; Loft, Steffen ; Møller, Peter. / Genotoxic potential of the perfluorinated chemicals PFOA, PFOS, PFBS, PFNA and PFHxA in human HepG2 cells. In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2010 ; Vol. 700, No. 1-2. pp. 39-43.

Bibtex

@article{d7320fe0e80f11dfb6d2000ea68e967b,
title = "Genotoxic potential of the perfluorinated chemicals PFOA, PFOS, PFBS, PFNA and PFHxA in human HepG2 cells",
abstract = "Synthetically produced perfluorinated chemicals (PFCs) are widely used in industrial products because of their anti-wetting and surfactant properties. PFCs are suspected carcinogens and a possible mechanism of action is generation of oxidative stress. We have investigated the potential of five different PFCs to generate reactive oxygen species (ROS) and to induce oxidative DNA damage in HepG2 cells. Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) increased the intracellular ROS production by 1.52-fold (95{\%} CI, 1.37-1.67) and 1.25-fold (95{\%} CI, 1.10-1.40), respectively. However, the increase in ROS production was not concentration-dependent and the compounds did not generate DNA damage that could be detected by the alkaline comet assay as strand breakage and alkali-labile sites or formamidopyrimidine-DNA-glycosylase (FPG) sites. Perfluorobutane sulfonate (PFBS) and perfluorohexanoic acid (PFHxA) did not generate ROS or DNA damage. Only the exposure to perfluorononanoic acid (PFNA) caused a modest increase in DNA damage at a cytotoxic concentration level, which was detected as lactate dehydrogenase (LDH) release into the cell medium. This was not related to ROS generation. Collectively, these results indicate that PFCs induce only modest effects in terms of ROS production and DNA damage in a cell line representing the human liver.",
author = "Eriksen, {Kirsten Thorup} and Ole Raaschou-Nielsen and Mette S{\o}rensen and Martin Roursgaard and Steffen Loft and Peter M{\o}ller",
note = "Keywords: Alkanesulfonic Acids; Comet Assay; DNA Damage; Environmental Monitoring; Fluorocarbons; Hep G2 Cells; Hexanoic Acids; Humans; Octanoic Acids; Oxidative Stress; Reactive Oxygen Species; Sulfonic Acids",
year = "2010",
doi = "10.1016/j.mrgentox.2010.04.024",
language = "English",
volume = "700",
pages = "39--43",
journal = "Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis",
issn = "0027-5107",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Genotoxic potential of the perfluorinated chemicals PFOA, PFOS, PFBS, PFNA and PFHxA in human HepG2 cells

AU - Eriksen, Kirsten Thorup

AU - Raaschou-Nielsen, Ole

AU - Sørensen, Mette

AU - Roursgaard, Martin

AU - Loft, Steffen

AU - Møller, Peter

N1 - Keywords: Alkanesulfonic Acids; Comet Assay; DNA Damage; Environmental Monitoring; Fluorocarbons; Hep G2 Cells; Hexanoic Acids; Humans; Octanoic Acids; Oxidative Stress; Reactive Oxygen Species; Sulfonic Acids

PY - 2010

Y1 - 2010

N2 - Synthetically produced perfluorinated chemicals (PFCs) are widely used in industrial products because of their anti-wetting and surfactant properties. PFCs are suspected carcinogens and a possible mechanism of action is generation of oxidative stress. We have investigated the potential of five different PFCs to generate reactive oxygen species (ROS) and to induce oxidative DNA damage in HepG2 cells. Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) increased the intracellular ROS production by 1.52-fold (95% CI, 1.37-1.67) and 1.25-fold (95% CI, 1.10-1.40), respectively. However, the increase in ROS production was not concentration-dependent and the compounds did not generate DNA damage that could be detected by the alkaline comet assay as strand breakage and alkali-labile sites or formamidopyrimidine-DNA-glycosylase (FPG) sites. Perfluorobutane sulfonate (PFBS) and perfluorohexanoic acid (PFHxA) did not generate ROS or DNA damage. Only the exposure to perfluorononanoic acid (PFNA) caused a modest increase in DNA damage at a cytotoxic concentration level, which was detected as lactate dehydrogenase (LDH) release into the cell medium. This was not related to ROS generation. Collectively, these results indicate that PFCs induce only modest effects in terms of ROS production and DNA damage in a cell line representing the human liver.

AB - Synthetically produced perfluorinated chemicals (PFCs) are widely used in industrial products because of their anti-wetting and surfactant properties. PFCs are suspected carcinogens and a possible mechanism of action is generation of oxidative stress. We have investigated the potential of five different PFCs to generate reactive oxygen species (ROS) and to induce oxidative DNA damage in HepG2 cells. Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) increased the intracellular ROS production by 1.52-fold (95% CI, 1.37-1.67) and 1.25-fold (95% CI, 1.10-1.40), respectively. However, the increase in ROS production was not concentration-dependent and the compounds did not generate DNA damage that could be detected by the alkaline comet assay as strand breakage and alkali-labile sites or formamidopyrimidine-DNA-glycosylase (FPG) sites. Perfluorobutane sulfonate (PFBS) and perfluorohexanoic acid (PFHxA) did not generate ROS or DNA damage. Only the exposure to perfluorononanoic acid (PFNA) caused a modest increase in DNA damage at a cytotoxic concentration level, which was detected as lactate dehydrogenase (LDH) release into the cell medium. This was not related to ROS generation. Collectively, these results indicate that PFCs induce only modest effects in terms of ROS production and DNA damage in a cell line representing the human liver.

U2 - 10.1016/j.mrgentox.2010.04.024

DO - 10.1016/j.mrgentox.2010.04.024

M3 - Journal article

VL - 700

SP - 39

EP - 43

JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

SN - 0027-5107

IS - 1-2

ER -

ID: 22929986