Effects of biochar amendments on antibiotic resistome of the soil and collembolan gut

Research output: Contribution to journalJournal articleResearchpeer-review

Jing Ding, Yue Yin, An Qi Sun, Simon Bo Lassen, Gang Li, Dong Zhu, Xin Ke

A diverse array of ARGs has been detected in the guts of soil fauna residing in farmland soil. Biochar has been widely used in farmland for soil remediation and improvement of soil quality; however, the effects of biochar amendment on the gut-associated ARGs of soil fauna remain unclear. In the present study, collembolans were cultivated in soils amended with 6 types of biochars. High-throughput qPCR was used to establish ARG profiles of the collembolan guts as well as the surrounding soils. A total of 73 and 162 subtypes of ARGs were detected in the collembolan guts and soils, respectively. Biochar amendment significantly altered the ARG compositions of the collembolan guts and soils, in a biochar quality-dependent manner. However, only manure-derived biochar, which contained elevated concentrations of heavy metals, increased the relative abundance of gut-associated ARGs. Changes in the gut microbial community, MGEs and biochar properties explained 84% of the total ARG variations in the collembolan guts. The findings of this study suggested that biochar properties should receive more attention, as high doses of heavy metals in biochar could increase the abundance of ARGs in collembolan guts, thereby contributing to the spread of ARGs in the environment through collembolan movement.

Original languageEnglish
JournalJournal of Hazardous Materials
Volume377
Pages (from-to)186-194
ISSN0304-3894
DOIs
Publication statusPublished - 5 Sep 2019

    Research areas

  • Biochar, Gut microbiota, Resistome, Soil fauna

ID: 223676063