Deletion of biosynthetic genes, specific SNP patterns and differences in transcript accumulation cause variation in hydroxynitrile glucoside content in barley cultivars

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Deletion of biosynthetic genes, specific SNP patterns and differences in transcript accumulation cause variation in hydroxynitrile glucoside content in barley cultivars. / Ehlert, Marcus; Jagd, Lea Møller; Braumann, Ilka; Dockter, Christoph; Crocoll, Christoph; Motawie, Mohammed Saddik; Møller, Birger Lindberg; Lyngkjær, Michael Foged.

In: Scientific Reports, Vol. 9, 5730, 05.04.2019, p. 1-10.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Ehlert, M, Jagd, LM, Braumann, I, Dockter, C, Crocoll, C, Motawie, MS, Møller, BL & Lyngkjær, MF 2019, 'Deletion of biosynthetic genes, specific SNP patterns and differences in transcript accumulation cause variation in hydroxynitrile glucoside content in barley cultivars', Scientific Reports, vol. 9, 5730, pp. 1-10. https://doi.org/10.1038/s41598-019-41884-w

APA

Ehlert, M., Jagd, L. M., Braumann, I., Dockter, C., Crocoll, C., Motawie, M. S., ... Lyngkjær, M. F. (2019). Deletion of biosynthetic genes, specific SNP patterns and differences in transcript accumulation cause variation in hydroxynitrile glucoside content in barley cultivars. Scientific Reports, 9, 1-10. [5730]. https://doi.org/10.1038/s41598-019-41884-w

Vancouver

Ehlert M, Jagd LM, Braumann I, Dockter C, Crocoll C, Motawie MS et al. Deletion of biosynthetic genes, specific SNP patterns and differences in transcript accumulation cause variation in hydroxynitrile glucoside content in barley cultivars. Scientific Reports. 2019 Apr 5;9:1-10. 5730. https://doi.org/10.1038/s41598-019-41884-w

Author

Ehlert, Marcus ; Jagd, Lea Møller ; Braumann, Ilka ; Dockter, Christoph ; Crocoll, Christoph ; Motawie, Mohammed Saddik ; Møller, Birger Lindberg ; Lyngkjær, Michael Foged. / Deletion of biosynthetic genes, specific SNP patterns and differences in transcript accumulation cause variation in hydroxynitrile glucoside content in barley cultivars. In: Scientific Reports. 2019 ; Vol. 9. pp. 1-10.

Bibtex

@article{c2085f702b94430eaf5e70b6880ccedf,
title = "Deletion of biosynthetic genes, specific SNP patterns and differences in transcript accumulation cause variation in hydroxynitrile glucoside content in barley cultivars",
abstract = "Barley (Hordeum vulgare L.) produces five leucine-derived hydroxynitrile glucosides, potentially involved in alleviating pathogen and environmental stresses. These compounds include the cyanogenic glucoside epiheterodendrin. The biosynthetic genes are clustered. Total hydroxynitrile glucoside contents were previously shown to vary from zero to more than 10,000 nmoles g−1 in different barley lines. To elucidate the cause of this variation, the biosynthetic genes from the high-level producer cv. Mentor, the medium-level producer cv. Pallas, and the zero-level producer cv. Emir were investigated. In cv. Emir, a major deletion in the genome spanning most of the hydroxynitrile glucoside biosynthetic gene cluster was identified and explains the complete absence of hydroxynitrile glucosides in this cultivar. The transcript levels of the biosynthetic genes were significantly higher in the high-level producer cv. Mentor compared to the medium-level producer cv. Pallas, indicating transcriptional regulation as a contributor to the variation in hydroxynitrile glucoside levels. A correlation between distinct single nucleotide polymorphism (SNP) patterns in the biosynthetic gene cluster and the hydroxynitrile glucoside levels in 227 barley lines was identified. It is remarkable that in spite of the demonstrated presence of a multitude of SNPs and differences in transcript levels, the ratio between the five hydroxynitrile glucosides is maintained across all the analysed barley lines. This implies the involvement of a stably assembled multienzyme complex.",
author = "Marcus Ehlert and Jagd, {Lea M{\o}ller} and Ilka Braumann and Christoph Dockter and Christoph Crocoll and Motawie, {Mohammed Saddik} and M{\o}ller, {Birger Lindberg} and Lyngkj{\ae}r, {Michael Foged}",
year = "2019",
month = "4",
day = "5",
doi = "https://doi.org/10.1038/s41598-019-41884-w",
language = "English",
volume = "9",
pages = "1--10",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Deletion of biosynthetic genes, specific SNP patterns and differences in transcript accumulation cause variation in hydroxynitrile glucoside content in barley cultivars

AU - Ehlert, Marcus

AU - Jagd, Lea Møller

AU - Braumann, Ilka

AU - Dockter, Christoph

AU - Crocoll, Christoph

AU - Motawie, Mohammed Saddik

AU - Møller, Birger Lindberg

AU - Lyngkjær, Michael Foged

PY - 2019/4/5

Y1 - 2019/4/5

N2 - Barley (Hordeum vulgare L.) produces five leucine-derived hydroxynitrile glucosides, potentially involved in alleviating pathogen and environmental stresses. These compounds include the cyanogenic glucoside epiheterodendrin. The biosynthetic genes are clustered. Total hydroxynitrile glucoside contents were previously shown to vary from zero to more than 10,000 nmoles g−1 in different barley lines. To elucidate the cause of this variation, the biosynthetic genes from the high-level producer cv. Mentor, the medium-level producer cv. Pallas, and the zero-level producer cv. Emir were investigated. In cv. Emir, a major deletion in the genome spanning most of the hydroxynitrile glucoside biosynthetic gene cluster was identified and explains the complete absence of hydroxynitrile glucosides in this cultivar. The transcript levels of the biosynthetic genes were significantly higher in the high-level producer cv. Mentor compared to the medium-level producer cv. Pallas, indicating transcriptional regulation as a contributor to the variation in hydroxynitrile glucoside levels. A correlation between distinct single nucleotide polymorphism (SNP) patterns in the biosynthetic gene cluster and the hydroxynitrile glucoside levels in 227 barley lines was identified. It is remarkable that in spite of the demonstrated presence of a multitude of SNPs and differences in transcript levels, the ratio between the five hydroxynitrile glucosides is maintained across all the analysed barley lines. This implies the involvement of a stably assembled multienzyme complex.

AB - Barley (Hordeum vulgare L.) produces five leucine-derived hydroxynitrile glucosides, potentially involved in alleviating pathogen and environmental stresses. These compounds include the cyanogenic glucoside epiheterodendrin. The biosynthetic genes are clustered. Total hydroxynitrile glucoside contents were previously shown to vary from zero to more than 10,000 nmoles g−1 in different barley lines. To elucidate the cause of this variation, the biosynthetic genes from the high-level producer cv. Mentor, the medium-level producer cv. Pallas, and the zero-level producer cv. Emir were investigated. In cv. Emir, a major deletion in the genome spanning most of the hydroxynitrile glucoside biosynthetic gene cluster was identified and explains the complete absence of hydroxynitrile glucosides in this cultivar. The transcript levels of the biosynthetic genes were significantly higher in the high-level producer cv. Mentor compared to the medium-level producer cv. Pallas, indicating transcriptional regulation as a contributor to the variation in hydroxynitrile glucoside levels. A correlation between distinct single nucleotide polymorphism (SNP) patterns in the biosynthetic gene cluster and the hydroxynitrile glucoside levels in 227 barley lines was identified. It is remarkable that in spite of the demonstrated presence of a multitude of SNPs and differences in transcript levels, the ratio between the five hydroxynitrile glucosides is maintained across all the analysed barley lines. This implies the involvement of a stably assembled multienzyme complex.

U2 - https://doi.org/10.1038/s41598-019-41884-w

DO - https://doi.org/10.1038/s41598-019-41884-w

M3 - Journal article

VL - 9

SP - 1

EP - 10

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 5730

ER -

ID: 216251844