Conservation of active ribosomes in acetone-treated cells of Tetrahymena pyriformis

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K Kristiansen, B Hartmann, P Plesner, A Krüger

The preparation of an acetone powder of cells of Tetrahymena pyriformis GL is described. A comparison of ribosomal particles isolated from acetone-treated and untreated cells shows that structurally and functionally intact ribosomes can be isolated from acetone-treated cells. Fully active ribosomes have been isolated from acetone powder of Tetrahymena that had been stored for more than 6 months at 4 degrees C. Thus, this procedure allows easy storage of large quantities of cells for the bulk preparation of active ribosomes.
Original languageEnglish
JournalEuropean Journal of Biochemistry
Volume83
Issue number2
Pages (from-to)389-94
Number of pages5
ISSN0014-2956
Publication statusPublished - 1978
Externally publishedYes

Bibliographical note

Keywords: Acetone; Animals; Cell Fractionation; Molecular Weight; Protein Biosynthesis; Ribosomal Proteins; Ribosomes; Tetrahymena pyriformis

ID: 11368600