Cholecystokinin inhibits gastrin secretion independently of paracrine somatostatin secretion in the pig

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Cholecystokinin inhibits gastrin secretion independently of paracrine somatostatin secretion in the pig. / Schmidt, P T; Hansen, L; Hilsted, L; Holst, Jens Juul.

In: Scandinavian Journal of Gastroenterology, Vol. 39, No. 3, 03.2004, p. 217-21.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Schmidt, PT, Hansen, L, Hilsted, L & Holst, JJ 2004, 'Cholecystokinin inhibits gastrin secretion independently of paracrine somatostatin secretion in the pig', Scandinavian Journal of Gastroenterology, vol. 39, no. 3, pp. 217-21.

APA

Schmidt, P. T., Hansen, L., Hilsted, L., & Holst, J. J. (2004). Cholecystokinin inhibits gastrin secretion independently of paracrine somatostatin secretion in the pig. Scandinavian Journal of Gastroenterology, 39(3), 217-21.

Vancouver

Schmidt PT, Hansen L, Hilsted L, Holst JJ. Cholecystokinin inhibits gastrin secretion independently of paracrine somatostatin secretion in the pig. Scandinavian Journal of Gastroenterology. 2004 Mar;39(3):217-21.

Author

Schmidt, P T ; Hansen, L ; Hilsted, L ; Holst, Jens Juul. / Cholecystokinin inhibits gastrin secretion independently of paracrine somatostatin secretion in the pig. In: Scandinavian Journal of Gastroenterology. 2004 ; Vol. 39, No. 3. pp. 217-21.

Bibtex

@article{ad031355b3ce486ea59bb4a2673823ab,
title = "Cholecystokinin inhibits gastrin secretion independently of paracrine somatostatin secretion in the pig",
abstract = "BACKGROUND: Cholecystokinin inhibits the secretion of gastrin from antral G cells, an effect that is speculated to be mediated by D cells secreting somatostatin. The aim of the study was to test directly whether cholecystokinin inhibition of antral gastrin secretion is mediated by somatostatin.METHODS: The effects of CCK on gastrin and somatostatin secretion were studied in isolated vascularly perfused preparations of pig antrum before and after immunoneutralization brought about by infusion of large amounts of a high affinity monoclonal antibody against somatostatin.RESULTS: CCK infusion at 10(-9) M and 10(-8) M decreased gastrin output to 70.5{\%} +/- 7.6{\%} (n = 8) and 76.3{\%} +/- 3.6{\%} (n = 7) of basal output, respectively. CCK at 10(-10) M had no effect (n = 6). Somatostatin secretion was dose-dependently increased by CCK infusion and increased to 268 +/- 38.2{\%} (n = 7) of basal secretion during infusion of CCK at 10(-8) M. Immunoneutralization of somatostatin caused a doubling of the basal secretion of gastrin, but did not affect the CCK-induced decrease in gastrin secretion.CONCLUSION: CCK inhibits gastrin secretion independently of paracrine somatostatin secretion.",
keywords = "Animals, Cholecystokinin, Gastrin-Secreting Cells, Gastrins, Paracrine Communication, Pyloric Antrum, Radioimmunoassay, Somatostatin, Somatostatin-Secreting Cells, Swine",
author = "Schmidt, {P T} and L Hansen and L Hilsted and Holst, {Jens Juul}",
year = "2004",
month = "3",
language = "English",
volume = "39",
pages = "217--21",
journal = "Scandinavian Journal of Gastroenterology",
issn = "0036-5521",
publisher = "Taylor & Francis",
number = "3",

}

RIS

TY - JOUR

T1 - Cholecystokinin inhibits gastrin secretion independently of paracrine somatostatin secretion in the pig

AU - Schmidt, P T

AU - Hansen, L

AU - Hilsted, L

AU - Holst, Jens Juul

PY - 2004/3

Y1 - 2004/3

N2 - BACKGROUND: Cholecystokinin inhibits the secretion of gastrin from antral G cells, an effect that is speculated to be mediated by D cells secreting somatostatin. The aim of the study was to test directly whether cholecystokinin inhibition of antral gastrin secretion is mediated by somatostatin.METHODS: The effects of CCK on gastrin and somatostatin secretion were studied in isolated vascularly perfused preparations of pig antrum before and after immunoneutralization brought about by infusion of large amounts of a high affinity monoclonal antibody against somatostatin.RESULTS: CCK infusion at 10(-9) M and 10(-8) M decreased gastrin output to 70.5% +/- 7.6% (n = 8) and 76.3% +/- 3.6% (n = 7) of basal output, respectively. CCK at 10(-10) M had no effect (n = 6). Somatostatin secretion was dose-dependently increased by CCK infusion and increased to 268 +/- 38.2% (n = 7) of basal secretion during infusion of CCK at 10(-8) M. Immunoneutralization of somatostatin caused a doubling of the basal secretion of gastrin, but did not affect the CCK-induced decrease in gastrin secretion.CONCLUSION: CCK inhibits gastrin secretion independently of paracrine somatostatin secretion.

AB - BACKGROUND: Cholecystokinin inhibits the secretion of gastrin from antral G cells, an effect that is speculated to be mediated by D cells secreting somatostatin. The aim of the study was to test directly whether cholecystokinin inhibition of antral gastrin secretion is mediated by somatostatin.METHODS: The effects of CCK on gastrin and somatostatin secretion were studied in isolated vascularly perfused preparations of pig antrum before and after immunoneutralization brought about by infusion of large amounts of a high affinity monoclonal antibody against somatostatin.RESULTS: CCK infusion at 10(-9) M and 10(-8) M decreased gastrin output to 70.5% +/- 7.6% (n = 8) and 76.3% +/- 3.6% (n = 7) of basal output, respectively. CCK at 10(-10) M had no effect (n = 6). Somatostatin secretion was dose-dependently increased by CCK infusion and increased to 268 +/- 38.2% (n = 7) of basal secretion during infusion of CCK at 10(-8) M. Immunoneutralization of somatostatin caused a doubling of the basal secretion of gastrin, but did not affect the CCK-induced decrease in gastrin secretion.CONCLUSION: CCK inhibits gastrin secretion independently of paracrine somatostatin secretion.

KW - Animals

KW - Cholecystokinin

KW - Gastrin-Secreting Cells

KW - Gastrins

KW - Paracrine Communication

KW - Pyloric Antrum

KW - Radioimmunoassay

KW - Somatostatin

KW - Somatostatin-Secreting Cells

KW - Swine

M3 - Journal article

VL - 39

SP - 217

EP - 221

JO - Scandinavian Journal of Gastroenterology

JF - Scandinavian Journal of Gastroenterology

SN - 0036-5521

IS - 3

ER -

ID: 132054559