Cell-penetrating antimicrobial peptides - prospectives for targeting intracellular infections

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Cell-penetrating antimicrobial peptides - prospectives for targeting intracellular infections. / Bahnsen, Jesper S; Franzyk, Henrik; Sayers, Edward J; Jones, Arwyn T; Nielsen, Hanne M.

In: Pharmaceutical Research, Vol. 32, No. 5, 2015, p. 1546-56.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Bahnsen, JS, Franzyk, H, Sayers, EJ, Jones, AT & Nielsen, HM 2015, 'Cell-penetrating antimicrobial peptides - prospectives for targeting intracellular infections', Pharmaceutical Research, vol. 32, no. 5, pp. 1546-56. https://doi.org/10.1007/s11095-014-1550-9

APA

Bahnsen, J. S., Franzyk, H., Sayers, E. J., Jones, A. T., & Nielsen, H. M. (2015). Cell-penetrating antimicrobial peptides - prospectives for targeting intracellular infections. Pharmaceutical Research, 32(5), 1546-56. https://doi.org/10.1007/s11095-014-1550-9

Vancouver

Bahnsen JS, Franzyk H, Sayers EJ, Jones AT, Nielsen HM. Cell-penetrating antimicrobial peptides - prospectives for targeting intracellular infections. Pharmaceutical Research. 2015;32(5):1546-56. https://doi.org/10.1007/s11095-014-1550-9

Author

Bahnsen, Jesper S ; Franzyk, Henrik ; Sayers, Edward J ; Jones, Arwyn T ; Nielsen, Hanne M. / Cell-penetrating antimicrobial peptides - prospectives for targeting intracellular infections. In: Pharmaceutical Research. 2015 ; Vol. 32, No. 5. pp. 1546-56.

Bibtex

@article{4c51746d782f465fa4f20e57fda51375,
title = "Cell-penetrating antimicrobial peptides - prospectives for targeting intracellular infections",
abstract = "PURPOSE: To investigate the suitability of three antimicrobial peptides (AMPs) as cell-penetrating antimicrobial peptides.METHODS: Cellular uptake of three AMPs (PK-12-KKP, SA-3 and TPk) and a cell-penetrating peptide (penetratin), all 5(6)-carboxytetramethylrhodamine-labeled, were tested in HeLa WT cells and analyzed by flow cytometry and confocal microscopy. Furthermore, the effects of the peptides on eukaryotic cell viability as well as their antimicrobial effect were tested. In addition, the disrupting ability of the peptides in the presence of bilayer membranes of different composition were analyzed.RESULTS: AMP uptake relative to penetratin was ~13{\%} (PK-12-KKP), ~66{\%} (SA-3) and ~50{\%} (TPk). All four peptides displayed a punctate uptake pattern in HeLa WT cells with co-localization to lysosomes and no indication that clathrin-mediated endocytosis was the predominant uptake mechanism. TPk showed the highest antibacterial activity. SA-3 exhibited selective disruption of liposomes mimicking Gram-positive and Gram-negative membranes.CONCLUSION: PK-12-KKP is an unlikely candidate for targeting intracellular bacteria, as the eukaryotic cell-penetrating ability is poor. SA-3, affected the cellular viability to an unacceptable degree. TPk showed acceptable uptake efficiency, high antimicrobial activity and relatively low toxicity, and it is the best potential lead peptide for further development.",
author = "Bahnsen, {Jesper S} and Henrik Franzyk and Sayers, {Edward J} and Jones, {Arwyn T} and Nielsen, {Hanne M}",
year = "2015",
doi = "10.1007/s11095-014-1550-9",
language = "English",
volume = "32",
pages = "1546--56",
journal = "Pharmaceutical Research",
issn = "0724-8741",
publisher = "Springer",
number = "5",

}

RIS

TY - JOUR

T1 - Cell-penetrating antimicrobial peptides - prospectives for targeting intracellular infections

AU - Bahnsen, Jesper S

AU - Franzyk, Henrik

AU - Sayers, Edward J

AU - Jones, Arwyn T

AU - Nielsen, Hanne M

PY - 2015

Y1 - 2015

N2 - PURPOSE: To investigate the suitability of three antimicrobial peptides (AMPs) as cell-penetrating antimicrobial peptides.METHODS: Cellular uptake of three AMPs (PK-12-KKP, SA-3 and TPk) and a cell-penetrating peptide (penetratin), all 5(6)-carboxytetramethylrhodamine-labeled, were tested in HeLa WT cells and analyzed by flow cytometry and confocal microscopy. Furthermore, the effects of the peptides on eukaryotic cell viability as well as their antimicrobial effect were tested. In addition, the disrupting ability of the peptides in the presence of bilayer membranes of different composition were analyzed.RESULTS: AMP uptake relative to penetratin was ~13% (PK-12-KKP), ~66% (SA-3) and ~50% (TPk). All four peptides displayed a punctate uptake pattern in HeLa WT cells with co-localization to lysosomes and no indication that clathrin-mediated endocytosis was the predominant uptake mechanism. TPk showed the highest antibacterial activity. SA-3 exhibited selective disruption of liposomes mimicking Gram-positive and Gram-negative membranes.CONCLUSION: PK-12-KKP is an unlikely candidate for targeting intracellular bacteria, as the eukaryotic cell-penetrating ability is poor. SA-3, affected the cellular viability to an unacceptable degree. TPk showed acceptable uptake efficiency, high antimicrobial activity and relatively low toxicity, and it is the best potential lead peptide for further development.

AB - PURPOSE: To investigate the suitability of three antimicrobial peptides (AMPs) as cell-penetrating antimicrobial peptides.METHODS: Cellular uptake of three AMPs (PK-12-KKP, SA-3 and TPk) and a cell-penetrating peptide (penetratin), all 5(6)-carboxytetramethylrhodamine-labeled, were tested in HeLa WT cells and analyzed by flow cytometry and confocal microscopy. Furthermore, the effects of the peptides on eukaryotic cell viability as well as their antimicrobial effect were tested. In addition, the disrupting ability of the peptides in the presence of bilayer membranes of different composition were analyzed.RESULTS: AMP uptake relative to penetratin was ~13% (PK-12-KKP), ~66% (SA-3) and ~50% (TPk). All four peptides displayed a punctate uptake pattern in HeLa WT cells with co-localization to lysosomes and no indication that clathrin-mediated endocytosis was the predominant uptake mechanism. TPk showed the highest antibacterial activity. SA-3 exhibited selective disruption of liposomes mimicking Gram-positive and Gram-negative membranes.CONCLUSION: PK-12-KKP is an unlikely candidate for targeting intracellular bacteria, as the eukaryotic cell-penetrating ability is poor. SA-3, affected the cellular viability to an unacceptable degree. TPk showed acceptable uptake efficiency, high antimicrobial activity and relatively low toxicity, and it is the best potential lead peptide for further development.

U2 - 10.1007/s11095-014-1550-9

DO - 10.1007/s11095-014-1550-9

M3 - Journal article

VL - 32

SP - 1546

EP - 1556

JO - Pharmaceutical Research

JF - Pharmaceutical Research

SN - 0724-8741

IS - 5

ER -

ID: 147069791