Blue ghosts: a new method for isolating amber mutants defective in essential genes of Escherichia coli

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S Brown, E R Brickman, J Beckwith

We describe a technique which permits an easy screening for amber mutants defective in essential genes of Escherichia coli. Using this approach, we have isolated three amber mutants defective in the rho gene. An extension of the technique allows the detection of ochre mutants and transposon insertions in essential genes.
Original languageEnglish
JournalJournal of Bacteriology
Volume146
Issue number1
Pages (from-to)422-5
Number of pages3
ISSN0021-9193
Publication statusPublished - 1981

Bibliographical note

Keywords: Escherichia coli; Genes; Genetic Techniques; Mutation

ID: 9298214