Jens-Christian Navarro Poulsen

Jens-Christian Navarro Poulsen

Academic staff


  1. 2019
  2. E-pub ahead of print

    Insights into an unusual Auxiliary Activity 9 family member lacking the histidine brace motif of lytic polysaccharide monooxygenases. / Frandsen, Kristian E H; Tovborg, Morten; Jørgensen, Christian I.; Spodsberg, Nikolai; Rosso, Marie-Noëlle; Hemsworth, Glyn R; Garman, Elspeth F; Grime, Geoffrey W; Poulsen, Jens-Christian N; Batth, Tanveer S.; Miyauchi, Shingo; Lipzen, Anna; Daum, Chris; Grigoriev, Igor V; Johansen, Katja S; Henrissat, Bernard; Berrin, Jean-Guy; Lo Leggio, Leila.

    In: The Journal of Biological Chemistry, 30.08.2019.

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Published

    Structure and Dynamics of a Promiscuous Xanthan Lyase from Paenibacillus nanensis and the Design of Variants with Increased Stability and Activity. / Jensen, Pernille Foged; Kadziola, Anders; Comamala, Gerard; Segura, Dorotea R; Anderson, Lars; Poulsen, Jens-Christian N; Rasmussen, Kim Krighaar; Agarwal, Shilpi; Sainathan, Rajendra K; Monrad, Rune Nygaard; Svendsen, Allan; Nielsen, Jens Erik; Lo Leggio, Leila; Rand, Kasper D.

    In: Cell Chemical Biology, Vol. 26, No. 2, 2019, p. 191-202.e1-e6.

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. 2018
  5. Published

    Biochemical and structural features of diverse bacterial glucuronoyl esterases facilitating recalcitrant biomass conversion. / Baath, Jenny Arnling; Mazurkewich, Scott; Knudsen, Rasmus Meland; Poulsen, Jens-Christian Navarro; Olsson, Lisbeth; Lo Leggio, Leila; Larsbrink, Johan.

    In: Biotechnology for Biofuels, Vol. 11, 213, 2018.

    Research output: Contribution to journalJournal articleResearchpeer-review

  6. Published

    Structure of a hyperthermostable carbonic anhydrase identified from an active hydrothermal vent chimney. / Fredslund, Folmer; Borchert, Martin S.; Poulsen, Jens-Christian Navarro; Mortensen, Steen Bennike; Perner, Mirjam; Streit, Wolfgang R.; Lo Leggio, Leila.

    In: Enzyme and Microbial Technology, Vol. 114, 2018, p. 48-54.

    Research output: Contribution to journalJournal articleResearchpeer-review

  7. Published

    Structure of a lytic polysaccharide monooxygenase from Aspergillus fumigatus and an engineered thermostable variant. / Lo Leggio, Leila; Weihe, Cecilia D.; Poulsen, Jens-christian N.; Sweeney, Matt; Rasmussen, Frank; Lin, Janine; De Maria, Leonardo; Wogulis, Mark.

    In: Carbohydrate Research, Vol. 469, 2018, p. 55-59.

    Research output: Contribution to journalJournal articleResearchpeer-review

  8. 2017
  9. Published

    Determination of protonation states of iminosugar-enzyme complexes using photoinduced electron transfer. / Wang, Bo; Olsen, Jacob Ingemar; Laursen, Bo W.; Poulsen, Jens Christian Navarro; Bols, Mikael.

    In: Chemical Science, Vol. 8, No. 11, 2017, p. 7383-7393.

    Research output: Contribution to journalJournal articleResearchpeer-review

  10. Published

    Learning from oligosaccharide soaks of crystals of an AA13 lytic polysaccharide monooxygenase : crystal packing, ligand binding and active-site disorder. / Frandsen, Kristian Erik Høpfner; Poulsen, Jens-Christian Navarro; Tovborg, Morten; Johansen, Katja Salomon; Lo Leggio, Leila.

    In: Acta crystallographica Section D: Structural biology , Vol. 73, 2017, p. 64-76.

    Research output: Contribution to journalJournal articleResearchpeer-review

  11. Published

    Structural and electronic determinants of lytic polysaccharide monooxygenase reactivity on polysaccharide substrates. / Simmons, T. J.; Frandsen, K. E. H.; Ciano, L.; Tryfona, T.; Lenfant, N.; Poulsen, J. C.; Wilson, L. F. L.; Tandrup, Tobias; Tovborg, M.; Schnorr, K.; Johansen, K. S.; Henrissat, B.; Walton, P. H.; Lo Leggio, L.; Dupree, P.

    In: Nature Communications, Vol. 8, 1064, 2017.

    Research output: Contribution to journalJournal articleResearchpeer-review

  12. Published

    Unliganded and substrate bound structures of the cellooligosaccharide active lytic polysaccharide monooxygenase LsAA9A at low pH. / Frandsen, Kristian Erik Høpfner; Poulsen, Jens-Christian Navarro; Tandrup, Tobias; Lo Leggio, Leila.

    In: Carbohydrate Research, Vol. 448, 2017, p. 187-190.

    Research output: Contribution to journalJournal articleResearchpeer-review

  13. 2016
  14. Published

    Structural characterization of the thermostable Bradyrhizobium japonicum D-sorbitol dehydrogenase. / Fredslund, Folmer; Otten, Harm; Gemperlein, Sabrina; Poulsen, Jens-Christian Navarro; Carius, Yvonne; Kohring, Gert-Wieland; Lo Leggio, Leila.

    In: Acta Crystallographica Section F, Vol. 72, No. 11, 2016, p. 846-852.

    Research output: Contribution to journalJournal articleResearchpeer-review

  15. Published

    The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases. / Frandsen, Kristian Erik Høpfner; Simmons, Thomas J.; Dupree, Paul; Poulsen, Jens-Christian Navarro; Hemsworth, Glyn R.; Ciano, Luisa; Johnston, Esther M.; Tovborg, Morten; Johansen, Katja Salomon; von Freiesleben, Pernille; Marmuse, Laurence; Fort, Sébastien; Cottaz, Sylvain; Driguez, Hugues; Henrissat, Bernard; Lenfant, Nicolas; Tuna, Floriana; Baldansuren, Amgalanbaatar; Davies, Gideon J.; Lo Leggio, Leila; Walton, Paul H.

    In: Nature Chemical Biology, Vol. 12, No. 4, 2016, p. 298-303.

    Research output: Contribution to journalJournal articleResearchpeer-review

  16. 2015
  17. Published

    A fluorescence study of isofagomine protonation in β-glucosidase. / Lindbäck, Emil Anders; Laursen, Bo Wegge; Poulsen, Jens-Christian Navarro; Kilså, Kristine; Pedersen, Christian Marcus; Bols, Mikael.

    In: Organic & Biomolecular Chemistry, Vol. 13, No. 23, 2015, p. 6562-6566.

    Research output: Contribution to journalJournal articleResearchpeer-review

  18. Published

    Adenine phosphoribosyltransferase from Sulfolobus solfataricus is an enzyme with unusual kinetic properties and a crystal structure that suggests it evolved from a 6-oxopurine phosphoribosyltransferase. / Jensen, Kaj Frank; Hansen, Michael Riis; Jensen, Kristine Steen; Christoffersen, Stig; Poulsen, Jens-Christian Navarro; Mølgaard, Anne; Kadziola, Anders.

    In: Biochemistry, Vol. 54, No. 14, 2015, p. 2323-2334.

    Research output: Contribution to journalJournal articleResearchpeer-review

  19. Published

    Structural diversity of lytic polysaccharide monooxygenases. / Lo Leggio, Leila; Frandsen, Kristian Erik Høpfner; Poulsen, Jens-Christian Navarro; Tovborg, Morten; De Maria, Leonardo; Johansen, Katja Salomon.

    Proceedings of "Mie Bioforum 2014 - Lignocellulose Degradation and Biorefinery". ed. / Kazuo Sakka; Tetsuya Kimura; Yutaka Tamaru; Shuichi Karita; Makiko Sakka. UNI Publishers Co., Ltd, 2015. p. 148-156.

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

  20. Published

    Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase. / Lo Leggio, Leila; Simmons, Thomas J.; Poulsen, Jens-Christian Navarro; Frandsen, Kristian Erik Høpfner; Hemsworth, Glyn R.; Stringer, Mary A.; von Freiesleben, Pernille; Tovborg, Morten; Johansen, Katja Salomon; De Maria, Leonardo; Harris, Paul V.; Soong, Chee-Leong; Dupree, Paul; Tryfona, Theodora; Lenfant, Nicolas; Henrissat, Bernard; Davies, Gideon J.; Walton, Paul H.

    In: Nature Communications, Vol. 6, 5961, 2015.

    Research output: Contribution to journalJournal articleResearchpeer-review

  21. 2014
  22. Published

    A new insight into the zinc-dependent DNA-cleavage by the colicin E7 nuclease : a crystallographic and computational study. / Czene, Anikó; Tóth, Eszter; Németh, Eszter; Otten, Harm; Poulsen, Jens-Christian Navarro; Christensen, Hans Erik Mølager; Rulíšek, Lubomír; Nagata, Kyosuke; Larsen, Sine; Gyurcsik, Béla.

    In: Metallomics, Vol. 6, No. 11, 2014, p. 2090-2099.

    Research output: Contribution to journalJournal articleResearchpeer-review

  23. Published

    Purification, crystal structure determination and functional characterization of type III antifreeze proteins from the European eelpout Zoarces viviparus. / Wilkens, Casper; Poulsen, Jens-Christian Navarro; Ramløv, Hans; Lo Leggio, Leila.

    In: Cryobiology, Vol. 69, No. 1, 2014, p. 163-168.

    Research output: Contribution to journalJournal articleResearchpeer-review

  24. 2013
  25. Published

    Binding of the N-terminal domain of the lactococcal bacteriophage TP901-1 CI repressor to its target DNA : a crystallography, small angle scattering, and nuclear magnetic resonance study. / Frandsen, Kristian Erik Høpfner; Rasmussen, Kim Krighaar; Jensen, Malene Ringkjøbing; Hammer, Karin; Pedersen, Margit; Poulsen, Jens-Christian Navarro; Arleth, Lise; Lo Leggio, Leila.

    In: Biochemistry, Vol. 52, No. 39, 2013, p. 6892-6904.

    Research output: Contribution to journalJournal articleResearchpeer-review

  26. Published

    Camel and bovine chymosin : the relationship between their structures and cheese-making properties. / Jensen, Jesper Langholm; Mølgaard, Anne; Poulsen, Jens-Christian Navarro; Harboe, Marianne Kirsten; Simonsen, Jens Bæk; Lorentzen, Andrea Maria; Hjernø, Karin; van den Brink, Johannes M.; Qvist, Karsten Bruun; Larsen, Sine.

    In: Acta Crystallographica. Section D: Biological Crystallography, Vol. D69, 2013, p. 901-913.

    Research output: Contribution to journalJournal articleResearchpeer-review

  27. Published

    Crystallization and preliminary crystallographic analysis of an Escherichia coli-selected mutant of the nuclease domain of the metallonuclease colicin E7. / Czene, Anikó; Toth, Eszter; Gyurcsik, Béla; Otten, Harm; Poulsen, Jens-Christian Navarro; Lo Leggio, Leila; Larsen, Sine; Christensen, Hans E.M.; Nagata, Kyosuke.

    In: Acta Crystallographica. Section F: Structural Biology and Crystallization Communications Online, Vol. 69, 2013, p. 551-554.

    Research output: Contribution to journalJournal articleResearchpeer-review

  28. 2012
  29. Published

    Enzymology and structure of the GH13_31 glucan 1,6-a-glucosidase that confers isomaltooligosaccharide utilization in the probiotic Lactobacillus acidophilus NCFM. / Møller, Marie Sofie; Fredslund, Folmer; Majumder, Avishek; Nakai, Hiroyuki; Poulsen, Jens-Christian Navarro; Lo Leggio, Leila; Svensson, Birte; Abou Hachem, Maher.

    In: Journal of Bacteriology, Vol. 194, No. 16, 2012, p. 4249-4259.

    Research output: Contribution to journalJournal articleResearchpeer-review

  30. 2011
  31. Published

    Activity of three ß-1,4-galactanases on small chromogenic substrates. / Torpenholt, Søs Katja; Le Nours, Jerome; Christensen, Ulla; Jahn, Michael; Withers, Stephen; Østergaard, Peter; Borchert, Torben V.; Poulsen, Jens-Christian Navarro; Lo Leggio, Leila.

    In: Carbohydrate Research, Vol. 346, 2011, p. 2028-2033.

    Research output: Contribution to journalJournal articleResearchpeer-review

  32. Published

    From screen to structure with a harvestable microfluidic device. / Stojanoff, V.; Jakoncic, J.; Oren, D. A.; Nagarajan, V.; Poulsen, Jens-Christian Navarro; Adams-Cioaba, M. A.; Bergfors, T.; Sommer, M. O. A.

    In: Acta Crystallographica. Section F: Structural Biology and Crystallization Communications Online, Vol. 67, No. 8, 2011, p. 971-975.

    Research output: Contribution to journalJournal articleResearchpeer-review

  33. Published

    Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components to cleave cellulose. / Quinlan, R. Jason; Sweeney, Matt D.; Lo Leggio, Leila; Otten, Harm; Poulsen, Jens-Christian Navarro; Johansen, Katja Salomon; Krogh, Kristian B. R. M.; Jørgensen, Christian Isak; Tovborg, Morten; Anthonsen, Annika; Tryfona, Theodora; Tryfona, Theodora; Walter, Clive P.; Dupree, Paul; Xu, Feng; Davies, Gideon J.; Walton, Paul H.

    In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, 2011, p. 15079-15084.

    Research output: Contribution to journalJournal articleResearchpeer-review

  34. Published

    Structure of a fungal dimeric a-type carbonic anhydrase. / Cuesta Seijo, Jose Antonio; Borchert, M. S.; Poulsen, Jens-Christian Navarro; Schnorr, K. M.; Mortensen, S. B.; Lo Leggio, Leila.

    In: F E B S Letters, Vol. 585, 2011, p. 1042-1048.

    Research output: Contribution to journalJournal articleResearchpeer-review

  35. 2010
  36. Published

    Stimulation of lignocellulosic biomass hydrolysis by proteins of glycoside hydrolase family 61: Structure and function of a large, enigmatic family. / Harris, Paul V; Welner, Ditte Hededam; McFarland, KC; Re, E; Poulsen, Jens-Christian Navarro; Brown, K; Salbo, Rune; Ding, H; Vlasenko, E; Marino, Sandy; Xu, F; Cherry, Joel; Larsen, Sine; Lo Leggio, Leila.

    In: Biochemistry, Vol. 49, 2010, p. 3305-3316.

    Research output: Contribution to journalJournal articleResearchpeer-review

  37. 2008
  38. Published
    Crystal Structure of NCAM2 Ig1 (I4122 Cell Unit). Kulahin, Nikolaj (Other); Rasmussen, Kim Krighaar (Other); Kristensen, Ole (Other); Kastrup, Jette Sandholm Jensen (Other); Poulsen, Jens-Christian Navarro (Other); Berezin, Vladimir (Other); Bock, Elisabeth Marianne (Other); Walmod, Peter Schledermann (Other); Gajhede, Michael (Other). 2008.

    Research output: Non-textual formDatasetResearch

  39. Published

    Crystal structure of the Ig1 domain of the neural cell adhesion molecule NCAM2 displays domain swapping. / Rasmussen, Kim Krighaar; Kulahin, Nikolaj; Kristensen, Ole; Poulsen, Jens-Christian N; Sigurskjold, Bent W; Kastrup, Jette S; Berezin, Vladimir; Bock, Elisabeth; Walmod, Peter S; Gajhede, Michael.

    In: Journal of Molecular Biology, Vol. 382, No. 5, 2008, p. 1113-20.

    Research output: Contribution to journalJournal articleResearchpeer-review

  40. Published

    Short strong hydrogen bonds in proteins: a case study of rhamnogalacturonan acetylesterase. / Langkilde, Annette Eva; Kristensen, Søren Martin; Lo Leggio, Leila; Mølgaard, Anne; Jensen, Jan Halborg; Houk, Andrew R.; Poulsen, Jens-Christian Navarro; Larsen, Sine Ydun; Kauppinen, S.

    In: Acta Crystallographica. Section D: Biological Crystallography, Vol. 64, 2008, p. 851-863.

    Research output: Contribution to journalJournal articleResearchpeer-review

  41. Published

    Structure and function of enzymes in biomass degradation. / Welner, Ditte Hededam; Jensen, Malene Hillerup; McFarland, Keith Charles; Poulsen, Jens-Christian Navarro; Otten, Harm; Salbo, Rune; Christensen, Ulla; Harris, Pernille; Larsen, Sine; Borchert, Torben; Christensen, Lars L. H.; Friis, Esben Peter; Lo Leggio, Leila.

    Biotechnology of lignocellulose degradation and Biomass Utilization - Mie Bioforum 2008. 2008. p. 349-355.

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearch

  42. 2004
  43. Published

    High-throughput physical characterization of protein solutions using microfluides. / Sommer, Morten Otto Alexander; Poulsen, Jens-Christian Navarro; Larsen, Sine.

    In: Acta Crystallographica, No. A60, 2004, p. 206.

    Research output: Contribution to journalJournal articleResearchpeer-review

  44. Published

    Rhamnogalacturonan lyase reveals a unique three-domain modular structure for polysaccharide lyase family 4. / McDonough, Michael A.; Kadirvelraj, Renuka; Harris, Pernille Hanne; Poulsen, Jens-Christian Navarro; Larsen, Sine.

    In: FEBS Letters, No. 565, 2004, p. 188-194.

    Research output: Contribution to journalJournal articleResearchpeer-review

  45. 2003
  46. Published

    Impact of the physical and chemical environment on the molecular structure of Coprinus cinereus peroxidase. / Houborg, Karen; Harris, Pernille Hanne; Petersen, Jens G.L.; Rowland, Paul; Poulsen, Jens-Christian Navarro; Schneider, Palle; Vind, Jesper; Larsen, Sine.

    In: Acta Crystallographica. Section D: Biological Crystallography, No. D59, 2003, p. 989-996.

    Research output: Contribution to journalJournal articleResearchpeer-review

  47. Published

    The Structure of a mutant enzyme of Coprinus cinereus peroxidase provides an understanding of its increased thermostability. / Houborg, Karen; Harris, Pernille Hanne; Poulsen, Jens-Christian Navarro; Schneider, Palle; Svendsen, Allan; Larsen, Sine.

    In: Acta Crystallographica. Section D: Biological Crystallography, No. D59, 2003, p. 997-1003.

    Research output: Contribution to journalJournal articleResearchpeer-review

  48. 2002
  49. Published

    A stepwise optimization of crystals of rhamnogalacturonan lyase from Aspergillus aculeatus. / Kadirvelraj, Renuka; Harris, Pernille Hanne; Poulsen, Jens-Christian Navarro; Kauppinen, Sakari; Larsen, Sine.

    In: Acta Crystallographica D, No. 58, 2002, p. 1346-1349.

    Research output: Contribution to journalJournal articleResearchpeer-review

  50. Published

    Substrate Binding Induces Domain Movements in Orotidine 5'-Monophosphate Decarboxylase. / Harris, Pernille Hanne; Poulsen, Jens-Christian Navarro; Jensen, Kaj Frank; Larsen, Sine.

    In: Journal of Molecular Biology, Vol. 318, No. 4, 2002, p. 1019-1029.

    Research output: Contribution to journalJournal articleResearchpeer-review

  51. 2001
  52. Published

    Selenomethionine substitution of orotidine-5'-monophosphate decarboxylase causes a change in crystal contacts and space group. / Poulsen, Jens-Christian Navarro; Harris, Pernille Hanne; Jensen, Kaj Frank; Larsen, Sine.

    In: Acta Crystallographica. Section D: Biological Crystallography, Vol. 57, No. 9, 2001, p. 1251-1259.

    Research output: Contribution to journalJournal articleResearchpeer-review

  53. Published

    Selenomethionine substitution of orotidine-5-monophosphate decarboxylase causes a change in crystal contacts and space group. / Poulsen, Jens-Christian Navarro; Harris, Pernille; Jensen, Kaj Frank; Larsen, Sine.

    In: Acta Crystallographica. Section D: Biological Crystallography, Vol. 57, No. 9, 2001, p. 1251-1259.

    Research output: Contribution to journalJournal articleResearchpeer-review

  54. Published

    Selenomethionine substitution of orotidine-5′-­monophosphate decarboxylase causes a change in crystal contacts and space group. / Poulsen, Jens-Christian Navarro; Harris, Pernille Hanne; Jensen, Kaj Frank; Larsen, Sine.

    In: Acta Crystallographica. Section D: Biological Crystallography, Vol. 57, No. 9, 2001, p. 1251-1259.

    Research output: Contribution to journalJournal articleResearchpeer-review

  55. Published

    pH, conductivity and long-term stability in the Crystal Screen solutions. / Bukrinski, Jens Thostrup; Poulsen, Jens-Christian Navarro.

    In: Journal of Applied Crystallography, Vol. 34, 2001, p. 533-534.

    Research output: Contribution to journalJournal articleResearch

  56. 2000
  57. Published

    Structural basis for the catalytic mechanism of a proficient enzyme: Orotidine 5'-Monophosphate Decarboxylase. / Harris, Pernille Hanne; Poulsen, Jens-Christian Navarro; Jensen, Kaj Frank; Larsen, Sine.

    In: Biochemistry, Vol. 39, No. 15, 2000, p. 4217-4224.

    Research output: Contribution to journalJournal articleResearchpeer-review

  58. 1999
  59. Published

    Crystallization and preliminary X-ray studies of β-1,4-galactanase from Aspergillus aculeatus. / Ryttersgaard, Carsten; Poulsen, Jens-Christian Juhl; Christgau, Stefan; Sandal, T.; Dalbøge, H.; Larsen, Sine.

    In: Acta Cryst., No. D55, 1999, p. 929-930.

    Research output: Contribution to journalJournal articleResearchpeer-review

  60. 1998
  61. Published

    Crystallization and preliminary X-ray diffraction studies of the heterogeneously glycosylated enzyme rhamnogalacturonan acetylesterase from Aspergillus aculeatus. / Mølgaard, Anne; Petersen, Jens F.W.; Kauppinen, Sakari; Dalbøge, Henrik; Johnsen, Anders H.; Poulsen, Jens-Christian Navarro; Larsen, Sine.

    In: Acta Crystallographica, Vol. D54, 1998, p. 1026-1029.

    Research output: Contribution to journalJournal articleResearchpeer-review

  62. 1995
  63. Published

    Thapsigargin-sensitive Ca2+-ATPases account for Ca2+ uptake to inositol 1,4,5-trisphosphate-sensitive and caffeine-sensitive Ca2+ stores in adrenal chromaffin cells. / Poulsen, Jens-Christian Navarro; Caspersen, C.; Mathiasen, D.; East, J.M.; Tunwell, R.E.A.; Lai, F.A.; Maedea, N.; Mikoshiba, K.; Treiman, M.

    In: Biochem. J., No. 307(3), 1995, p. 749-758.

    Research output: Contribution to journalJournal articleResearch

  64. 1994
  65. Published

    Ca2+-ATPase inhibitory activity of a locked analog of thapsigargin. / Andersen, A.; Treiman, M.; Poulsen, Jens-Christian Navarro; Cornett, C.; Moldt, P.; Olsen, C.E.; Christensen, S.B.

    In: Bioorg. Med. Chem. Lett., No. 4(5), 1994, p. 657-660.

    Research output: Contribution to journalJournal articleResearchpeer-review

  66. 1993
  67. Published

    Derivatives of thapsigargin as probes of its binding-site on endoplasmicreticulum Ca2+ ATPase stereoselectivity and important functional groups. / Christensen, S.B.; Andersen, A.; Poulsen, Jens-Christian Navarro; Treiman, M.

    In: FEBS Letters, No. 335(3), 1993, p. 345-348.

    Research output: Contribution to journalJournal articleResearchpeer-review

ID: 8112