The role of RecQ helicases in non-homologous end-joining

Research output: Contribution to journalReviewResearchpeer-review

Guido Keijzers, Scott Maynard, Raghavendra A Shamanna, Lene Juel Rasmussen, Deborah L Croteau, Vilhelm A Bohr

Abstract DNA double-strand breaks are highly toxic DNA lesions that cause genomic instability, if not efficiently repaired. RecQ helicases are a family of highly conserved proteins that maintain genomic stability through their important roles in several DNA repair pathways, including DNA double-strand break repair. Double-strand breaks can be repaired by homologous recombination (HR) using sister chromatids as templates to facilitate precise DNA repair, or by an HR-independent mechanism known as non-homologous end-joining (NHEJ) (error-prone). NHEJ is a non-templated DNA repair process, in which DNA termini are directly ligated. Canonical NHEJ requires DNA-PKcs and Ku70/80, while alternative NHEJ pathways are DNA-PKcs and Ku70/80 independent. This review discusses the role of RecQ helicases in NHEJ, alternative (or back-up) NHEJ (B-NHEJ) and microhomology-mediated end-joining (MMEJ) in V(D)J recombination, class switch recombination and telomere maintenance.

Original languageEnglish
JournalCritical Reviews in Biochemistry and Molecular Biology
Volume49
Issue number6
Pages (from-to)463-472
Number of pages10
ISSN1040-9238
DOIs
Publication statusPublished - Nov 2014

ID: 119407677