The role of mitochondrial dNTP levels in cells with reduced TK2 activity

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The role of mitochondrial dNTP levels in cells with reduced TK2 activity. / Desler, C; Munch-Petersen, B; Rasmussen, L J.

In: Nucleosides, nucleotides & nucleic acids, Vol. 25, No. 9-11, 2006, p. 1171-5.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Desler, C, Munch-Petersen, B & Rasmussen, LJ 2006, 'The role of mitochondrial dNTP levels in cells with reduced TK2 activity', Nucleosides, nucleotides & nucleic acids, vol. 25, no. 9-11, pp. 1171-5. https://doi.org/10.1080/15257770600894501

APA

Desler, C., Munch-Petersen, B., & Rasmussen, L. J. (2006). The role of mitochondrial dNTP levels in cells with reduced TK2 activity. Nucleosides, nucleotides & nucleic acids, 25(9-11), 1171-5. https://doi.org/10.1080/15257770600894501

Vancouver

Desler C, Munch-Petersen B, Rasmussen LJ. The role of mitochondrial dNTP levels in cells with reduced TK2 activity. Nucleosides, nucleotides & nucleic acids. 2006;25(9-11):1171-5. https://doi.org/10.1080/15257770600894501

Author

Desler, C ; Munch-Petersen, B ; Rasmussen, L J. / The role of mitochondrial dNTP levels in cells with reduced TK2 activity. In: Nucleosides, nucleotides & nucleic acids. 2006 ; Vol. 25, No. 9-11. pp. 1171-5.

Bibtex

@article{9e5139513c1a48d4815fbf7445441f69,

title = "The role of mitochondrial dNTP levels in cells with reduced TK2 activity",

abstract = "Both the nuclear and mitochondrial DNA (mtDNA) depend on separate balanced pools of dNTPs for correct function of DNA replication and repair of DNA damage. Import of dNTPs from the cytosolic compartment to the mitochondria has been suggested to have the potential of rectifying a mitochondrial dNTP imbalance. Reduced TK2 activity has been demonstrated to result in mitochondrial dNTP imbalance and consequently mutations of mtDNA in non-dividing cells. In this study, the consequences of a reduced thymidine kinase 2 (TK2) activity were measured in proliferating HeLa cells, on both whole-cell as well as mitochondrial dNTP levels. With the exception of increased mitochondrial dCTP level no significant difference was found in cells with reduced TK2 activity. Our results suggest that import of cytosolic dNTPs in mitochondria of proliferating cells can compensate a TK2 induced imbalance of the mitochondrial dNTP pool.",

keywords = "Analysis of Variance, Biological Transport, Cell Proliferation, Cytosol, DNA Damage, DNA, Mitochondrial, Deoxyribonucleotides, Gene Expression Regulation, Enzymologic, HeLa Cells, Humans, Mitochondria, Plasmids, Thymidine Kinase",

author = "C Desler and B Munch-Petersen and Rasmussen, {L J}",

year = "2006",

doi = "10.1080/15257770600894501",

language = "English",

volume = "25",

pages = "1171--5",

journal = "Nucleosides, Nucleotides and Nucleic Acids",

issn = "1525-7770",

publisher = "Taylor & Francis",

number = "9-11",

}

RIS

TY - JOUR

T1 - The role of mitochondrial dNTP levels in cells with reduced TK2 activity

AU - Desler, C

AU - Munch-Petersen, B

AU - Rasmussen, L J

PY - 2006

Y1 - 2006

N2 - Both the nuclear and mitochondrial DNA (mtDNA) depend on separate balanced pools of dNTPs for correct function of DNA replication and repair of DNA damage. Import of dNTPs from the cytosolic compartment to the mitochondria has been suggested to have the potential of rectifying a mitochondrial dNTP imbalance. Reduced TK2 activity has been demonstrated to result in mitochondrial dNTP imbalance and consequently mutations of mtDNA in non-dividing cells. In this study, the consequences of a reduced thymidine kinase 2 (TK2) activity were measured in proliferating HeLa cells, on both whole-cell as well as mitochondrial dNTP levels. With the exception of increased mitochondrial dCTP level no significant difference was found in cells with reduced TK2 activity. Our results suggest that import of cytosolic dNTPs in mitochondria of proliferating cells can compensate a TK2 induced imbalance of the mitochondrial dNTP pool.

AB - Both the nuclear and mitochondrial DNA (mtDNA) depend on separate balanced pools of dNTPs for correct function of DNA replication and repair of DNA damage. Import of dNTPs from the cytosolic compartment to the mitochondria has been suggested to have the potential of rectifying a mitochondrial dNTP imbalance. Reduced TK2 activity has been demonstrated to result in mitochondrial dNTP imbalance and consequently mutations of mtDNA in non-dividing cells. In this study, the consequences of a reduced thymidine kinase 2 (TK2) activity were measured in proliferating HeLa cells, on both whole-cell as well as mitochondrial dNTP levels. With the exception of increased mitochondrial dCTP level no significant difference was found in cells with reduced TK2 activity. Our results suggest that import of cytosolic dNTPs in mitochondria of proliferating cells can compensate a TK2 induced imbalance of the mitochondrial dNTP pool.

KW - Analysis of Variance

KW - Biological Transport

KW - Cell Proliferation

KW - Cytosol

KW - DNA Damage

KW - DNA, Mitochondrial

KW - Deoxyribonucleotides

KW - Gene Expression Regulation, Enzymologic

KW - HeLa Cells

KW - Humans

KW - Mitochondria

KW - Plasmids

KW - Thymidine Kinase

U2 - 10.1080/15257770600894501

DO - 10.1080/15257770600894501

M3 - Journal article

C2 - 17065084

VL - 25

SP - 1171

EP - 1175

JO - Nucleosides, Nucleotides and Nucleic Acids

JF - Nucleosides, Nucleotides and Nucleic Acids

SN - 1525-7770

IS - 9-11

ER -

ID: 119639915