The behaviour of fibroblasts migrating from chick heart explants: changes in adhesion, locomotion and growth, and in the distribution of actomyosin and fibronectin.

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J R Couchman, D A Rees

Fibroblasts migrating from heart explants of chick embryos at first have a high rate of locomotion but lack focal contacts or adhesions and also lack substantial actin-containing bundles. A meshwork of 7-nm filaments is present particularly in submembranous regions and is proposed to be directed towards efficient locomotion whilst maintaining a high degree of spreading. Also during the first 48 h there is little production of extracellular fibronectin and the growth rate is low. Later, these fibroblasts develop focal contacts and focal adhesions together with actomyosin bundles, with a parallel increase in fibronectin expression. We propose that progressive immobilization by the development of focal adhesions and actomyosin structures occurs to set these cells up for growth.
Original languageEnglish
JournalJournal of Cell Science
Volume39
Pages (from-to)149-65
Number of pages16
ISSN0021-9533
Publication statusPublished - 1979

Bibliographical note

Keywords: Actomyosin; Animals; Cell Adhesion; Cell Cycle; Cell Division; Cell Membrane; Cell Movement; Chick Embryo; Cytoskeleton; Fibroblasts; Fibronectins; Myocardium

ID: 5167990