Targeted 3' processing of antisense transcripts triggers Arabidopsis FLC chromatin silencing
Research output: Contribution to journal › Journal article › peer-review
Noncoding RNA is emerging as an important regulator of gene expression in many organisms. We are characterizing RNA-mediated chromatin silencing of the Arabidopsis major floral repressor gene, FLC. Through suppressor mutagenesis, we identify a requirement for CstF64 and CstF77, two conserved RNA 3'-end-processing factors, in FLC silencing. However, FLC sense transcript 3' processing is not affected in the mutants. Instead, CstF64 and CstF77 are required for 3' processing of FLC antisense transcripts. A specific RNA-binding protein directs their activity to a proximal antisense polyadenylation site. This targeted processing triggers localized histone demethylase activity and results in reduced FLC sense transcription. Targeted 3' processing of antisense transcripts may be a common mechanism triggering transcriptional silencing of the corresponding sense gene.
Original language | English |
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Journal | Science (New York, N.Y.) |
Volume | 327 |
Issue number | 5961 |
Pages (from-to) | 94-7 |
Number of pages | 4 |
ISSN | 0036-8075 |
DOIs | |
Publication status | Published - 2010 |
Externally published | Yes |
- Arabidopsis, Arabidopsis Proteins, Chromatin, Cleavage Stimulation Factor, Epistasis, Genetic, Flowers, Gene Expression Regulation, Plant, Gene Silencing, Histone Deacetylases, MADS Domain Proteins, Models, Genetic, Polyadenylation, RNA Processing, Post-Transcriptional, RNA, Antisense, RNA, Plant, RNA-Binding Proteins, Suppression, Genetic, Transcription, Genetic, mRNA Cleavage and Polyadenylation Factors, Journal Article, Research Support, Non-U.S. Gov't
Research areas
ID: 183164664