Syndecan-2 is a novel ligand for the protein tyrosine phosphatase receptor CD148

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Syndecan-2 is a novel ligand for the protein tyrosine phosphatase receptor CD148. / Whiteford, James R; Xian, Xiaojie; Chaussade, Claire; Vanhaesebroeck, Bart; Nourshargh, Sussan; Couchman, John R.

In: Molecular Biology of the Cell, Vol. 22, No. 19, 2011, p. 3609-24.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Whiteford, JR, Xian, X, Chaussade, C, Vanhaesebroeck, B, Nourshargh, S & Couchman, JR 2011, 'Syndecan-2 is a novel ligand for the protein tyrosine phosphatase receptor CD148', Molecular Biology of the Cell, vol. 22, no. 19, pp. 3609-24. https://doi.org/10.1091/mbc.E11-02-0099

APA

Whiteford, J. R., Xian, X., Chaussade, C., Vanhaesebroeck, B., Nourshargh, S., & Couchman, J. R. (2011). Syndecan-2 is a novel ligand for the protein tyrosine phosphatase receptor CD148. Molecular Biology of the Cell, 22(19), 3609-24. https://doi.org/10.1091/mbc.E11-02-0099

Vancouver

Whiteford JR, Xian X, Chaussade C, Vanhaesebroeck B, Nourshargh S, Couchman JR. Syndecan-2 is a novel ligand for the protein tyrosine phosphatase receptor CD148. Molecular Biology of the Cell. 2011;22(19):3609-24. https://doi.org/10.1091/mbc.E11-02-0099

Author

Whiteford, James R ; Xian, Xiaojie ; Chaussade, Claire ; Vanhaesebroeck, Bart ; Nourshargh, Sussan ; Couchman, John R. / Syndecan-2 is a novel ligand for the protein tyrosine phosphatase receptor CD148. In: Molecular Biology of the Cell. 2011 ; Vol. 22, No. 19. pp. 3609-24.

Bibtex

@article{ec0210dfc11842f38402df4df89cd925,
title = "Syndecan-2 is a novel ligand for the protein tyrosine phosphatase receptor CD148",
abstract = "Syndecan-2 is a heparan sulfate proteoglycan that has a cell adhesion regulatory domain contained within its extracellular core protein. Cell adhesion to the syndecan-2 extracellular domain (S2ED) is {\ss}1 integrin dependent; however, syndecan-2 is not an integrin ligand. Here the protein tyrosine phosphatase receptor CD148 is shown to be a key intermediary in cell adhesion to S2ED, with downstream {\ss}1 integrin-mediated adhesion and cytoskeletal organization. We show that S2ED is a novel ligand for CD148 and identify the region proximal to the transmembrane domain of syndecan-2 as the site of interaction with CD148. A mechanism for the transduction of the signal from CD148 to {\ss}1 integrins is elucidated requiring Src kinase and potential implication of the C2{\ss} isoform of phosphatidylinositol 3 kinase. Our data uncover a novel pathway for {\ss}1 integrin-mediated adhesion of importance in cellular processes such as angiogenesis and inflammation.",
keywords = "Animals, Antigens, CD29, Cell Adhesion, Cell Line, Cytoskeleton, Fibroblasts, Gene Expression Regulation, Humans, Inflammation, Jurkat Cells, Ligands, Lung, Mice, Neovascularization, Physiologic, Phosphatidylinositol 3-Kinase, Protein Interaction Domains and Motifs, RNA, Small Interfering, Rats, Receptor-Like Protein Tyrosine Phosphatases, Class 3, Signal Transduction, Syndecan-2, src-Family Kinases",
author = "Whiteford, {James R} and Xiaojie Xian and Claire Chaussade and Bart Vanhaesebroeck and Sussan Nourshargh and Couchman, {John R}",
year = "2011",
doi = "10.1091/mbc.E11-02-0099",
language = "English",
volume = "22",
pages = "3609--24",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "19",

}

RIS

TY - JOUR

T1 - Syndecan-2 is a novel ligand for the protein tyrosine phosphatase receptor CD148

AU - Whiteford, James R

AU - Xian, Xiaojie

AU - Chaussade, Claire

AU - Vanhaesebroeck, Bart

AU - Nourshargh, Sussan

AU - Couchman, John R

PY - 2011

Y1 - 2011

N2 - Syndecan-2 is a heparan sulfate proteoglycan that has a cell adhesion regulatory domain contained within its extracellular core protein. Cell adhesion to the syndecan-2 extracellular domain (S2ED) is ß1 integrin dependent; however, syndecan-2 is not an integrin ligand. Here the protein tyrosine phosphatase receptor CD148 is shown to be a key intermediary in cell adhesion to S2ED, with downstream ß1 integrin-mediated adhesion and cytoskeletal organization. We show that S2ED is a novel ligand for CD148 and identify the region proximal to the transmembrane domain of syndecan-2 as the site of interaction with CD148. A mechanism for the transduction of the signal from CD148 to ß1 integrins is elucidated requiring Src kinase and potential implication of the C2ß isoform of phosphatidylinositol 3 kinase. Our data uncover a novel pathway for ß1 integrin-mediated adhesion of importance in cellular processes such as angiogenesis and inflammation.

AB - Syndecan-2 is a heparan sulfate proteoglycan that has a cell adhesion regulatory domain contained within its extracellular core protein. Cell adhesion to the syndecan-2 extracellular domain (S2ED) is ß1 integrin dependent; however, syndecan-2 is not an integrin ligand. Here the protein tyrosine phosphatase receptor CD148 is shown to be a key intermediary in cell adhesion to S2ED, with downstream ß1 integrin-mediated adhesion and cytoskeletal organization. We show that S2ED is a novel ligand for CD148 and identify the region proximal to the transmembrane domain of syndecan-2 as the site of interaction with CD148. A mechanism for the transduction of the signal from CD148 to ß1 integrins is elucidated requiring Src kinase and potential implication of the C2ß isoform of phosphatidylinositol 3 kinase. Our data uncover a novel pathway for ß1 integrin-mediated adhesion of importance in cellular processes such as angiogenesis and inflammation.

KW - Animals

KW - Antigens, CD29

KW - Cell Adhesion

KW - Cell Line

KW - Cytoskeleton

KW - Fibroblasts

KW - Gene Expression Regulation

KW - Humans

KW - Inflammation

KW - Jurkat Cells

KW - Ligands

KW - Lung

KW - Mice

KW - Neovascularization, Physiologic

KW - Phosphatidylinositol 3-Kinase

KW - Protein Interaction Domains and Motifs

KW - RNA, Small Interfering

KW - Rats

KW - Receptor-Like Protein Tyrosine Phosphatases, Class 3

KW - Signal Transduction

KW - Syndecan-2

KW - src-Family Kinases

U2 - 10.1091/mbc.E11-02-0099

DO - 10.1091/mbc.E11-02-0099

M3 - Journal article

C2 - 21813734

VL - 22

SP - 3609

EP - 3624

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 19

ER -

ID: 38429768