Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins

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Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins. / Hawkins, Clare Louise; Rees, Martin D; Davies, Michael Jonathan.

In: FEBS letters, Vol. 510, No. 1-2, 02.01.2002, p. 41-4.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Hawkins, CL, Rees, MD & Davies, MJ 2002, 'Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins', FEBS letters, vol. 510, no. 1-2, pp. 41-4.

APA

Hawkins, C. L., Rees, M. D., & Davies, M. J. (2002). Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins. FEBS letters, 510(1-2), 41-4.

Vancouver

Hawkins CL, Rees MD, Davies MJ. Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins. FEBS letters. 2002 Jan 2;510(1-2):41-4.

Author

Hawkins, Clare Louise ; Rees, Martin D ; Davies, Michael Jonathan. / Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins. In: FEBS letters. 2002 ; Vol. 510, No. 1-2. pp. 41-4.

Bibtex

@article{05fea4836d4a4393affeecff86103e67,
title = "Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins",
abstract = "Activated phagocytes generate both superoxide radicals via a respiratory burst, and HOCl via the concurrent release of the haem enzyme myeloperoxidase. Amine and amide functions on proteins and carbohydrates are major targets for HOCl, generating chloramines (RNHCl) and chloramides (RC(O)NClR'), which can accumulate to high concentrations (>100 microM). Here we show that superoxide radicals catalyse the decomposition of chloramines and chloramides to reactive nitrogen-centred radicals, and increase the extent of protein fragmentation compared to that observed with either superoxide radicals or HOCl, alone. This synergistic action may be of significance at sites of inflammation, where both superoxide radicals and chloramines/chloramides are formed simultaneously.",
keywords = "Chloramines, Drug Synergism, Hypochlorous Acid, Proteins, Reactive Nitrogen Species, Serum Albumin, Bovine, Superoxides",
author = "Hawkins, {Clare Louise} and Rees, {Martin D} and Davies, {Michael Jonathan}",
year = "2002",
month = "1",
day = "2",
language = "English",
volume = "510",
pages = "41--4",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd",
number = "1-2",

}

RIS

TY - JOUR

T1 - Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins

AU - Hawkins, Clare Louise

AU - Rees, Martin D

AU - Davies, Michael Jonathan

PY - 2002/1/2

Y1 - 2002/1/2

N2 - Activated phagocytes generate both superoxide radicals via a respiratory burst, and HOCl via the concurrent release of the haem enzyme myeloperoxidase. Amine and amide functions on proteins and carbohydrates are major targets for HOCl, generating chloramines (RNHCl) and chloramides (RC(O)NClR'), which can accumulate to high concentrations (>100 microM). Here we show that superoxide radicals catalyse the decomposition of chloramines and chloramides to reactive nitrogen-centred radicals, and increase the extent of protein fragmentation compared to that observed with either superoxide radicals or HOCl, alone. This synergistic action may be of significance at sites of inflammation, where both superoxide radicals and chloramines/chloramides are formed simultaneously.

AB - Activated phagocytes generate both superoxide radicals via a respiratory burst, and HOCl via the concurrent release of the haem enzyme myeloperoxidase. Amine and amide functions on proteins and carbohydrates are major targets for HOCl, generating chloramines (RNHCl) and chloramides (RC(O)NClR'), which can accumulate to high concentrations (>100 microM). Here we show that superoxide radicals catalyse the decomposition of chloramines and chloramides to reactive nitrogen-centred radicals, and increase the extent of protein fragmentation compared to that observed with either superoxide radicals or HOCl, alone. This synergistic action may be of significance at sites of inflammation, where both superoxide radicals and chloramines/chloramides are formed simultaneously.

KW - Chloramines

KW - Drug Synergism

KW - Hypochlorous Acid

KW - Proteins

KW - Reactive Nitrogen Species

KW - Serum Albumin, Bovine

KW - Superoxides

M3 - Journal article

VL - 510

SP - 41

EP - 44

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 1-2

ER -

ID: 138279041