Stable markers of oxidant damage to proteins and their application in the study of human disease
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Stable markers of oxidant damage to proteins and their application in the study of human disease. / Davies, Michael Jonathan; Fu, S; Wang, H; Dean, R T.
In: Free Radical Biology & Medicine, Vol. 27, No. 11-12, 12.1999, p. 1151-63.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Stable markers of oxidant damage to proteins and their application in the study of human disease
AU - Davies, Michael Jonathan
AU - Fu, S
AU - Wang, H
AU - Dean, R T
PY - 1999/12
Y1 - 1999/12
N2 - The mechanisms of formation and the nature of the altered amino acid side chains formed on proteins subjected to oxidant attack are reviewed. The use of stable products of protein side chain oxidation as potential markers for assessing oxidative damage in vivo in humans is discussed. The methods developed in the authors laboratories are outlined, and the advantages and disadvantages of these techniques compared with other methodologies for assessing oxidative damage to proteins and other macromolecules. Evidence is presented to show that protein oxidation products are sensitive markers of oxidative damage, that the pattern of products detected may yield information as to the nature of the original oxidative insult, and that the levels of oxidized side-chains can, in certain circumstances, be much higher than those of other markers of oxidation such as lipid hydroperoxides.
AB - The mechanisms of formation and the nature of the altered amino acid side chains formed on proteins subjected to oxidant attack are reviewed. The use of stable products of protein side chain oxidation as potential markers for assessing oxidative damage in vivo in humans is discussed. The methods developed in the authors laboratories are outlined, and the advantages and disadvantages of these techniques compared with other methodologies for assessing oxidative damage to proteins and other macromolecules. Evidence is presented to show that protein oxidation products are sensitive markers of oxidative damage, that the pattern of products detected may yield information as to the nature of the original oxidative insult, and that the levels of oxidized side-chains can, in certain circumstances, be much higher than those of other markers of oxidation such as lipid hydroperoxides.
KW - Amino Acids
KW - Animals
KW - Biological Markers
KW - Disease
KW - Humans
KW - Oxidants
KW - Oxidation-Reduction
KW - Oxidative Stress
KW - Proteins
M3 - Journal article
C2 - 10641706
VL - 27
SP - 1151
EP - 1163
JO - Free Radical Biology & Medicine
JF - Free Radical Biology & Medicine
SN - 0891-5849
IS - 11-12
ER -
ID: 138282023