Serum protein oxidation and apolipoprotein CIII levels in people with systemic lupus erythematosus with and without nephritis

Research output: Contribution to journalJournal articleResearchpeer-review

Philip E Morgan, Allan D Sturgess, Annemarie Hennessy, Michael Jonathan Davies

Increased oxidative stress is a hallmark of the autoimmune disease systemic lupus erythematosus (SLE). This study compares serum protein oxidation levels in SLE patients without and with renal involvement (lupus nephritis); the latter have a significantly poorer prognosis. Similar increases in protein carbonyls and decreases in protein thiols were observed in both SLE groups compared to controls. Protein carbonyl distribution, determined by Western blotting of 2D gels, was similar in both SLE groups, suggesting factors other than oxidation also play a role in SLE complications. 2D electrophoresis examined the serum proteome further. Six proteins were significantly decreases in non-renal SLE patients compared to controls; five were identified by mass spectrometry, including one isoform of pro-atherogenic apoCIII. Total apoCIII levels (assessed by ELISA) in lupus nephritis patients were significantly elevated compared to controls or non-renal SLE patients. Thus, levels of oxidized proteins and apoCIII may be useful biomarkers in SLE studies.

Original languageEnglish
JournalFree Radical Research
Volume41
Issue number12
Pages (from-to)1301-12
Number of pages12
ISSN1071-5762
DOIs
Publication statusPublished - Dec 2007
Externally publishedYes

    Research areas

  • Adult, Apolipoprotein C-III, Biological Markers, Blood Proteins, Electrophoresis, Gel, Two-Dimensional, Enzyme-Linked Immunosorbent Assay, Female, Humans, Lupus Erythematosus, Systemic, Male, Middle Aged, Nephritis, Oxidation-Reduction, Proteome

ID: 129671117