Serum protein oxidation and apolipoprotein CIII levels in people with systemic lupus erythematosus with and without nephritis
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Serum protein oxidation and apolipoprotein CIII levels in people with systemic lupus erythematosus with and without nephritis. / Morgan, Philip E; Sturgess, Allan D; Hennessy, Annemarie; Davies, Michael Jonathan.
In: Free Radical Research, Vol. 41, No. 12, 12.2007, p. 1301-12.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Serum protein oxidation and apolipoprotein CIII levels in people with systemic lupus erythematosus with and without nephritis
AU - Morgan, Philip E
AU - Sturgess, Allan D
AU - Hennessy, Annemarie
AU - Davies, Michael Jonathan
PY - 2007/12
Y1 - 2007/12
N2 - Increased oxidative stress is a hallmark of the autoimmune disease systemic lupus erythematosus (SLE). This study compares serum protein oxidation levels in SLE patients without and with renal involvement (lupus nephritis); the latter have a significantly poorer prognosis. Similar increases in protein carbonyls and decreases in protein thiols were observed in both SLE groups compared to controls. Protein carbonyl distribution, determined by Western blotting of 2D gels, was similar in both SLE groups, suggesting factors other than oxidation also play a role in SLE complications. 2D electrophoresis examined the serum proteome further. Six proteins were significantly decreases in non-renal SLE patients compared to controls; five were identified by mass spectrometry, including one isoform of pro-atherogenic apoCIII. Total apoCIII levels (assessed by ELISA) in lupus nephritis patients were significantly elevated compared to controls or non-renal SLE patients. Thus, levels of oxidized proteins and apoCIII may be useful biomarkers in SLE studies.
AB - Increased oxidative stress is a hallmark of the autoimmune disease systemic lupus erythematosus (SLE). This study compares serum protein oxidation levels in SLE patients without and with renal involvement (lupus nephritis); the latter have a significantly poorer prognosis. Similar increases in protein carbonyls and decreases in protein thiols were observed in both SLE groups compared to controls. Protein carbonyl distribution, determined by Western blotting of 2D gels, was similar in both SLE groups, suggesting factors other than oxidation also play a role in SLE complications. 2D electrophoresis examined the serum proteome further. Six proteins were significantly decreases in non-renal SLE patients compared to controls; five were identified by mass spectrometry, including one isoform of pro-atherogenic apoCIII. Total apoCIII levels (assessed by ELISA) in lupus nephritis patients were significantly elevated compared to controls or non-renal SLE patients. Thus, levels of oxidized proteins and apoCIII may be useful biomarkers in SLE studies.
KW - Adult
KW - Apolipoprotein C-III
KW - Biological Markers
KW - Blood Proteins
KW - Electrophoresis, Gel, Two-Dimensional
KW - Enzyme-Linked Immunosorbent Assay
KW - Female
KW - Humans
KW - Lupus Erythematosus, Systemic
KW - Male
KW - Middle Aged
KW - Nephritis
KW - Oxidation-Reduction
KW - Proteome
U2 - 10.1080/10715760701684809
DO - 10.1080/10715760701684809
M3 - Journal article
C2 - 17957542
VL - 41
SP - 1301
EP - 1312
JO - Free Radical Research
JF - Free Radical Research
SN - 1071-5762
IS - 12
ER -
ID: 129671117