Removal of amino acid, peptide and protein hydroperoxides by reaction with peroxiredoxins 2 and 3

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Alexander V Peskin, Andrew G Cox, Péter Nagy, Philip E Morgan, Mark B Hampton, Michael Jonathan Davies, Christine C Winterbourn

Prxs (peroxiredoxins) are a ubiquitous family of cysteine-dependent peroxidases that react rapidly with H2O2 and alkyl hydroperoxides and provide defence against these reactive oxidants. Hydroperoxides are also formed on amino acids and proteins during oxidative stress, and they too are a potential cause of biological damage. We have investigated whether Prxs react with amino acid, peptide and protein hydroperoxides, and whether the reactions are sufficiently rapid for these enzymes to provide antioxidant protection against these oxidants. Isolated Prx2, which is a cytosolic protein, and Prx3, which resides within mitochondria, were reacted with a selection of hydroperoxides generated by γ-radiolysis or singlet oxygen, on free amino acids, peptides and proteins. Reactions were followed by measuring the accumulation of disulfide-linked Prx dimers, via non-reducing SDS/PAGE, or the loss of the corresponding hydroperoxide, using quench-flow and LC (liquid chromatography)/MS. All the hydroperoxides induced rapid oxidation, with little difference in reactivity between Prx2 and Prx3. N-acetyl leucine hydroperoxides reacted with Prx2 with a rate constant of 4 × 10(4) M-1 · s-1. Hydroperoxides present on leucine, isoleucine or tyrosine reacted at a comparable rate, whereas histidine hydroperoxides were ~10-fold less reactive. Hydroperoxides present on lysozyme and BSA reacted with rate constants of ~100 M-1 · s-1. Addition of an uncharged derivative of leucine hydroperoxide to intact erythrocytes caused Prx2 oxidation with no concomitant loss in GSH, as did BSA hydroperoxide when added to concentrated erythrocyte lysate. Prxs are therefore favoured intracellular targets for peptide/protein hydroperoxides and have the potential to detoxify these species in vivo.

Original languageEnglish
JournalBiochemical Journal
Volume432
Issue number2
Pages (from-to)313-21
Number of pages9
ISSN0264-6021
DOIs
Publication statusPublished - 1 Dec 2010
Externally publishedYes

    Research areas

  • Amino Acids, Dipeptides, Erythrocytes, Gamma Rays, Glutathione, Humans, Hydrogen Peroxide, Isoleucine, Leucine, Oxidative Stress, Peptides, Peroxides, Peroxiredoxin III, Peroxiredoxins, Singlet Oxygen

ID: 129669928