Pulmonary exposure to particles from diesel exhaust, urban dust or single-walled carbon nanotubes and oxidatively damaged DNA and vascular function in apoE(-/-)mice
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Pulmonary exposure to particles from diesel exhaust, urban dust or single-walled carbon nanotubes and oxidatively damaged DNA and vascular function in apoE(-/-)mice. / Vesterdal, Lise K; Jantzen, Kim; Sheykhzade, Majid; Roursgaard, Martin; Folkmann, Janne K; Loft, Steffen; Møller, Peter.
In: Nanotoxicology, Vol. 8, No. 1, 14.11.2012, p. 61-71.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Pulmonary exposure to particles from diesel exhaust, urban dust or single-walled carbon nanotubes and oxidatively damaged DNA and vascular function in apoE(-/-)mice
AU - Vesterdal, Lise K
AU - Jantzen, Kim
AU - Sheykhzade, Majid
AU - Roursgaard, Martin
AU - Folkmann, Janne K
AU - Loft, Steffen
AU - Møller, Peter
N1 - Editor-in-Chief: Prof. Håkan Wallin 2011 Impact Factor: 5.758 5-Year Impact Factor: 6.781 Eight issues are published per year. ISSN: 1743-5390 (print), 1743-5404 (electronic) Read More: http://informahealthcare.com/nan
PY - 2012/11/14
Y1 - 2012/11/14
N2 - Abstract This study compared the oxidative stress level and vasomotor dysfunction after exposure to urban dust, diesel exhaust particles (DEP) or single-walled carbon nanotubes (SWCNT). DEP and SWCNT increased the production of reactive oxygen species (ROS) in cultured endothelial cells and acellullarly, whereas the exposure to urban dust did not generate ROS. ApoE(-/-) mice, which were exposed twice to 0.5 mg/kg of the particles by intratracheal instillation, had unaltered acetylcholine-elicited vasorelaxation in aorta segments. There was unaltered pulmonary expression level of Vcam-1, Icam-1, Hmox-1 and Ogg1. The levels of oxidatively damaged DNA were unchanged in lung tissue. The exposure to SWCNT significantly increased the expression of Ccl-2 in the lung tissue of the mice. The exposure to DEP and SWCNT was associated with elevated ROS production in cultured cells, whereas intratracheal instillation of the same particles had no effect on biomarkers of pulmonary oxidative stress and dilatory dysfunction in the aorta.
AB - Abstract This study compared the oxidative stress level and vasomotor dysfunction after exposure to urban dust, diesel exhaust particles (DEP) or single-walled carbon nanotubes (SWCNT). DEP and SWCNT increased the production of reactive oxygen species (ROS) in cultured endothelial cells and acellullarly, whereas the exposure to urban dust did not generate ROS. ApoE(-/-) mice, which were exposed twice to 0.5 mg/kg of the particles by intratracheal instillation, had unaltered acetylcholine-elicited vasorelaxation in aorta segments. There was unaltered pulmonary expression level of Vcam-1, Icam-1, Hmox-1 and Ogg1. The levels of oxidatively damaged DNA were unchanged in lung tissue. The exposure to SWCNT significantly increased the expression of Ccl-2 in the lung tissue of the mice. The exposure to DEP and SWCNT was associated with elevated ROS production in cultured cells, whereas intratracheal instillation of the same particles had no effect on biomarkers of pulmonary oxidative stress and dilatory dysfunction in the aorta.
U2 - 10.3109/17435390.2012.750385
DO - 10.3109/17435390.2012.750385
M3 - Journal article
C2 - 23148895
VL - 8
SP - 61
EP - 71
JO - Nanotoxicology
JF - Nanotoxicology
SN - 1743-5390
IS - 1
ER -
ID: 41898893