Pulmonary exposure to carbon black by inhalation or instillation in pregnant mice: effects on liver DNA strand breaks in dams and offspring

Research output: Contribution to journalJournal articleResearchpeer-review

Petra Jackson, Karin Sørig Hougaard, Anne Mette Zenner Boisen, Nicklas Raun Jacobsen, Keld Alstrup Jensen, Peter Møller, Gunnar Brunborg, Kristine Bjerve Gutzkow, Ole Andersen, Steffen Loft, Ulla Birgitte Vogel, Håkan Wallin

Effects of maternal pulmonary exposure to carbon black (Printex 90) on gestation, lactation and DNA strand breaks were evaluated. Time-mated C57BL/6BomTac mice were exposed by inhalation to 42 mg/m(3) Printex 90 for 1 h/day on gestation days (GD) 8-18, or by four intratracheal instillations on GD 7, 10, 15 and 18, with total doses of 11, 54 and 268 µg/animal. Dams were monitored until weaning and some offspring until adolescence. Inflammation was assessed in maternal bronchoalveolar lavage (BAL) 3-5 days after exposure, and at weaning. Levels of DNA strand breaks were assessed in maternal BAL cells and liver, and in offspring liver. Persistent lung inflammation was observed in exposed mothers. Inhalation exposure induced more DNA strand breaks in the liver of mothers and their offspring, whereas intratracheal instillation did not. Neither inhalation nor instillation affected gestation and lactation. Maternal inhalation exposure to Printex 90-induced liver DNA damage in the mothers and the in utero exposed offspring.
Original languageEnglish
JournalNanotoxicology
Volume6
Issue number5
Pages (from-to)486-500
Number of pages15
ISSN1743-5390
DOIs
Publication statusPublished - Aug 2012

    Research areas

  • Administration, Inhalation, Animals, Bronchoalveolar Lavage Fluid, DNA Damage, Drug-Induced Liver Injury, Female, Inhalation Exposure, Lactation, Liver, Male, Maternal Exposure, Mice, Mice, Inbred C57BL, Nanoparticles, Organ Size, Pneumonia, Pregnancy, Soot

ID: 45158626