Overproduction of PIB-Type ATPases
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Understanding of the functions and mechanisms of fundamental processes in the cell requires structural information. Structural studies of membrane proteins typically necessitate large amounts of purified and preferably homogenous target protein. Here, we describe a rapid overproduction and purification strategy of a bacterial PIB-type ATPase for isolation of milligrams of target protein per liter Escherichia coli cell culture, with a final quality of the sample which is sufficient for generating high-resolution crystals.
Original language | English |
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Title of host publication | P-Type ATPases : Methods and Protocols |
Editors | Maike Bublitz |
Number of pages | 8 |
Volume | 1377 |
Publisher | Springer |
Publication date | 2016 |
Pages | 29-36 |
Chapter | 5 |
ISBN (Print) | 978-1-4939-3178-1 |
ISBN (Electronic) | 978-1-4939-3179-8 |
DOIs | |
Publication status | Published - 2016 |
Series | Methods in Molecular Biology |
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Volume | 1377 |
ISSN | 1064-3745 |
ID: 171590537