Overproduction of PIB-Type ATPases

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Xiangyu Liu, Oleg Sitsel, Kaituo Wang, Pontus Gourdon

Understanding of the functions and mechanisms of fundamental processes in the cell requires structural information. Structural studies of membrane proteins typically necessitate large amounts of purified and preferably homogenous target protein. Here, we describe a rapid overproduction and purification strategy of a bacterial PIB-type ATPase for isolation of milligrams of target protein per liter Escherichia coli cell culture, with a final quality of the sample which is sufficient for generating high-resolution crystals.

Original languageEnglish
Title of host publicationP-Type ATPases : Methods and Protocols
EditorsMaike Bublitz
Number of pages8
Volume1377
PublisherSpringer
Publication date2016
Pages29-36
Chapter5
ISBN (Print)978-1-4939-3178-1
ISBN (Electronic)978-1-4939-3179-8
DOIs
Publication statusPublished - 2016
SeriesMethods in Molecular Biology
Volume1377
ISSN1064-3745

ID: 171590537