Mitochondrial oxidative phosphorylation capacity of cryopreserved cells

Research output: Contribution to journalJournal articleResearchpeer-review

Poul Ejner Lauridsen, Lene Juel Rasmussen, Claus Desler

Defects in mitochondrial oxidative phosphorylation are a feature of many human diseases. To date, determination of oxidative phosphorylation has required fresh and live sample material and therefore also access to specialized equipment and trained personnel. Cryopreservation of samples is an attractive alternative, where samples can be collected and stored in an economic and practical fashion for later bulk assays. Here, we present an accurate, reliable method for estimating mitochondrial oxidative phosphorylation capacity of cryopreserved human cells. Broad adoption of this method will allow uncomplicated collection of samples and measurements of oxidative phosphorylation.

Original languageEnglish
JournalMitochondrion
Volume47
Pages (from-to)47-53
Number of pages7
ISSN1567-7249
DOIs
Publication statusPublished - 2019

    Research areas

  • Bioenergetics, Cryopreservation, Immuno-detection, Oxidative phosphorylation

ID: 226792163