Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies.

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J R Couchman, A V Ljubimov

A panel of nine monoclonal antibodies has been characterized, all of which have reactivity with the core protein of a large heparan sulfate proteoglycan derived from the murine EHS tumor matrix. These rat monoclonal antibodies stained mouse basement membranes intensely, including those of all muscle, endothelia, peripheral nerve fibers and epithelia so far examined. In addition, two of the monoclonal antibodies show cross-species reactivity, staining bovine and human basement membranes, and immunoprecipitating proteoglycans from human endothelial cell cultures. These antibodies do not, however, cross-react with avian tissues. These results show the ubiquitous distribution of a heparan sulfate proteoglycan in mammalian tissues, which will be useful in vitro and in vivo for studies on the biology of basement membrane proteoglycans and investigations of possible roles of these molecules in human disease processes.
Original languageEnglish
JournalMatrix (Stuttgart, Germany)
Volume9
Issue number4
Pages (from-to)311-21
Number of pages10
ISSN0934-8832
Publication statusPublished - 1989

Bibliographical note

Keywords: Animals; Antibodies, Monoclonal; Antibody Specificity; Basement Membrane; Cattle; Epitopes; Extracellular Matrix; Fluorescent Antibody Technique; Heparin; Histocytochemistry; Humans; Kidney; Mice; Muscles; Proteoglycans; Rats; Tissue Distribution; Umbilical Veins

ID: 5167215