Hypobromous acid and bromamine production by neutrophils and modulation by superoxide

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Hypobromous acid and bromamine production by neutrophils and modulation by superoxide. / Chapman, Anna L P; Skaff, Ojia; Senthilmohan, Revathy; Kettle, Anthony J; Davies, Michael Jonathan.

In: Biochemical Journal, Vol. 417, No. 3, 01.02.2009, p. 773-81.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Chapman, ALP, Skaff, O, Senthilmohan, R, Kettle, AJ & Davies, MJ 2009, 'Hypobromous acid and bromamine production by neutrophils and modulation by superoxide', Biochemical Journal, vol. 417, no. 3, pp. 773-81. https://doi.org/10.1042/BJ20071563

APA

Chapman, A. L. P., Skaff, O., Senthilmohan, R., Kettle, A. J., & Davies, M. J. (2009). Hypobromous acid and bromamine production by neutrophils and modulation by superoxide. Biochemical Journal, 417(3), 773-81. https://doi.org/10.1042/BJ20071563

Vancouver

Chapman ALP, Skaff O, Senthilmohan R, Kettle AJ, Davies MJ. Hypobromous acid and bromamine production by neutrophils and modulation by superoxide. Biochemical Journal. 2009 Feb 1;417(3):773-81. https://doi.org/10.1042/BJ20071563

Author

Chapman, Anna L P ; Skaff, Ojia ; Senthilmohan, Revathy ; Kettle, Anthony J ; Davies, Michael Jonathan. / Hypobromous acid and bromamine production by neutrophils and modulation by superoxide. In: Biochemical Journal. 2009 ; Vol. 417, No. 3. pp. 773-81.

Bibtex

@article{6c89d71a507547c9b7403feb20b13618,
title = "Hypobromous acid and bromamine production by neutrophils and modulation by superoxide",
abstract = "MPO (myeloperoxidase) catalyses the oxidation of chloride, bromide and thiocyanate to their respective hypohalous acids. We have investigated the generation of HOBr by human neutrophils in the presence of physiological concentrations of chloride and bromide. HOBr was trapped with taurine and detected by monitoring the bromination of 4-HPAA (4-hydroxyphenylacetic acid). With 100 microM bromide and 140 mM chloride, neutrophils generated HOBr and it accounted for approx. 13{\%} of the hypohalous acids they produced. Addition of SOD (superoxide dismutase) doubled the amount of HOBr detected. Therefore we investigated the reaction of superoxide radicals with a range of bromamines and bromamides and found that superoxide radicals stimulated the decomposition of these species, with this occurring in a time- and dose-dependent manner. The protection afforded by SOD against such decay demonstrates that these processes are superoxide-radical-dependent. These data are consistent with neutrophils generating HOBr at sites of infection and inflammation. Both HOBr and bromamines/bromamides have the potential to react with superoxide radicals to form additional radicals that may contribute to inflammatory tissue damage.",
keywords = "Bromates, Bromides, Humans, Neutrophils, Phenylacetates, Spectrometry, Mass, Electrospray Ionization, Superoxide Dismutase, Superoxides, Taurine",
author = "Chapman, {Anna L P} and Ojia Skaff and Revathy Senthilmohan and Kettle, {Anthony J} and Davies, {Michael Jonathan}",
year = "2009",
month = "2",
day = "1",
doi = "10.1042/BJ20071563",
language = "English",
volume = "417",
pages = "773--81",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",
number = "3",

}

RIS

TY - JOUR

T1 - Hypobromous acid and bromamine production by neutrophils and modulation by superoxide

AU - Chapman, Anna L P

AU - Skaff, Ojia

AU - Senthilmohan, Revathy

AU - Kettle, Anthony J

AU - Davies, Michael Jonathan

PY - 2009/2/1

Y1 - 2009/2/1

N2 - MPO (myeloperoxidase) catalyses the oxidation of chloride, bromide and thiocyanate to their respective hypohalous acids. We have investigated the generation of HOBr by human neutrophils in the presence of physiological concentrations of chloride and bromide. HOBr was trapped with taurine and detected by monitoring the bromination of 4-HPAA (4-hydroxyphenylacetic acid). With 100 microM bromide and 140 mM chloride, neutrophils generated HOBr and it accounted for approx. 13% of the hypohalous acids they produced. Addition of SOD (superoxide dismutase) doubled the amount of HOBr detected. Therefore we investigated the reaction of superoxide radicals with a range of bromamines and bromamides and found that superoxide radicals stimulated the decomposition of these species, with this occurring in a time- and dose-dependent manner. The protection afforded by SOD against such decay demonstrates that these processes are superoxide-radical-dependent. These data are consistent with neutrophils generating HOBr at sites of infection and inflammation. Both HOBr and bromamines/bromamides have the potential to react with superoxide radicals to form additional radicals that may contribute to inflammatory tissue damage.

AB - MPO (myeloperoxidase) catalyses the oxidation of chloride, bromide and thiocyanate to their respective hypohalous acids. We have investigated the generation of HOBr by human neutrophils in the presence of physiological concentrations of chloride and bromide. HOBr was trapped with taurine and detected by monitoring the bromination of 4-HPAA (4-hydroxyphenylacetic acid). With 100 microM bromide and 140 mM chloride, neutrophils generated HOBr and it accounted for approx. 13% of the hypohalous acids they produced. Addition of SOD (superoxide dismutase) doubled the amount of HOBr detected. Therefore we investigated the reaction of superoxide radicals with a range of bromamines and bromamides and found that superoxide radicals stimulated the decomposition of these species, with this occurring in a time- and dose-dependent manner. The protection afforded by SOD against such decay demonstrates that these processes are superoxide-radical-dependent. These data are consistent with neutrophils generating HOBr at sites of infection and inflammation. Both HOBr and bromamines/bromamides have the potential to react with superoxide radicals to form additional radicals that may contribute to inflammatory tissue damage.

KW - Bromates

KW - Bromides

KW - Humans

KW - Neutrophils

KW - Phenylacetates

KW - Spectrometry, Mass, Electrospray Ionization

KW - Superoxide Dismutase

KW - Superoxides

KW - Taurine

U2 - 10.1042/BJ20071563

DO - 10.1042/BJ20071563

M3 - Journal article

VL - 417

SP - 773

EP - 781

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 3

ER -

ID: 129670548