EphB/syndecan-2 signaling in dendritic spine morphogenesis.

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

EphB/syndecan-2 signaling in dendritic spine morphogenesis. / Ethell, I M; Irie, F; Kalo, M S; Couchman, J R; Pasquale, E B; Yamaguchi, Y.

In: Neuron, Vol. 31, No. 6, 2001, p. 1001-13.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ethell, IM, Irie, F, Kalo, MS, Couchman, JR, Pasquale, EB & Yamaguchi, Y 2001, 'EphB/syndecan-2 signaling in dendritic spine morphogenesis.', Neuron, vol. 31, no. 6, pp. 1001-13.

APA

Ethell, I. M., Irie, F., Kalo, M. S., Couchman, J. R., Pasquale, E. B., & Yamaguchi, Y. (2001). EphB/syndecan-2 signaling in dendritic spine morphogenesis. Neuron, 31(6), 1001-13.

Vancouver

Ethell IM, Irie F, Kalo MS, Couchman JR, Pasquale EB, Yamaguchi Y. EphB/syndecan-2 signaling in dendritic spine morphogenesis. Neuron. 2001;31(6):1001-13.

Author

Ethell, I M ; Irie, F ; Kalo, M S ; Couchman, J R ; Pasquale, E B ; Yamaguchi, Y. / EphB/syndecan-2 signaling in dendritic spine morphogenesis. In: Neuron. 2001 ; Vol. 31, No. 6. pp. 1001-13.

Bibtex

@article{c9bc7a30596e11dd8d9f000ea68e967b,
title = "EphB/syndecan-2 signaling in dendritic spine morphogenesis.",
abstract = "We previously reported that the cell surface proteoglycan syndecan-2 can induce dendritic spine formation in hippocampal neurons. We demonstrate here that the EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. Syndecan-2 is tyrosine phosphorylated and forms a complex with EphB2 in mouse brain. Dominant-negative inhibition of endogenous EphB receptor activities blocks clustering of endogenous syndecan-2 and normal spine formation in cultured hippocampal neurons. This is the first evidence that Eph receptors play a physiological role in dendritic spine morphogenesis. Our observations suggest that spine morphogenesis is triggered by the activation of Eph receptors, which causes tyrosine phosphorylation of target molecules, such as syndecan-2, in presumptive spines.",
author = "Ethell, {I M} and F Irie and Kalo, {M S} and Couchman, {J R} and Pasquale, {E B} and Y Yamaguchi",
note = "Keywords: Animals; Cells, Cultured; Dendrites; Excitatory Postsynaptic Potentials; Hippocampus; Membrane Glycoproteins; Mice; Morphogenesis; Mutagenesis, Site-Directed; Nerve Tissue Proteins; Neuronal Plasticity; Phosphorylation; Phosphotyrosine; Protein Processing, Post-Translational; Proteoglycans; Rats; Receptor Protein-Tyrosine Kinases; Receptor, EphB2; Signal Transduction; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Syndecan-2; Transfection",
year = "2001",
language = "English",
volume = "31",
pages = "1001--13",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "6",

}

RIS

TY - JOUR

T1 - EphB/syndecan-2 signaling in dendritic spine morphogenesis.

AU - Ethell, I M

AU - Irie, F

AU - Kalo, M S

AU - Couchman, J R

AU - Pasquale, E B

AU - Yamaguchi, Y

N1 - Keywords: Animals; Cells, Cultured; Dendrites; Excitatory Postsynaptic Potentials; Hippocampus; Membrane Glycoproteins; Mice; Morphogenesis; Mutagenesis, Site-Directed; Nerve Tissue Proteins; Neuronal Plasticity; Phosphorylation; Phosphotyrosine; Protein Processing, Post-Translational; Proteoglycans; Rats; Receptor Protein-Tyrosine Kinases; Receptor, EphB2; Signal Transduction; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Syndecan-2; Transfection

PY - 2001

Y1 - 2001

N2 - We previously reported that the cell surface proteoglycan syndecan-2 can induce dendritic spine formation in hippocampal neurons. We demonstrate here that the EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. Syndecan-2 is tyrosine phosphorylated and forms a complex with EphB2 in mouse brain. Dominant-negative inhibition of endogenous EphB receptor activities blocks clustering of endogenous syndecan-2 and normal spine formation in cultured hippocampal neurons. This is the first evidence that Eph receptors play a physiological role in dendritic spine morphogenesis. Our observations suggest that spine morphogenesis is triggered by the activation of Eph receptors, which causes tyrosine phosphorylation of target molecules, such as syndecan-2, in presumptive spines.

AB - We previously reported that the cell surface proteoglycan syndecan-2 can induce dendritic spine formation in hippocampal neurons. We demonstrate here that the EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. Syndecan-2 is tyrosine phosphorylated and forms a complex with EphB2 in mouse brain. Dominant-negative inhibition of endogenous EphB receptor activities blocks clustering of endogenous syndecan-2 and normal spine formation in cultured hippocampal neurons. This is the first evidence that Eph receptors play a physiological role in dendritic spine morphogenesis. Our observations suggest that spine morphogenesis is triggered by the activation of Eph receptors, which causes tyrosine phosphorylation of target molecules, such as syndecan-2, in presumptive spines.

M3 - Journal article

C2 - 11580899

VL - 31

SP - 1001

EP - 1013

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 6

ER -

ID: 5162904