Direct detection and quantification of transition metal ions in human atherosclerotic plaques: evidence for the presence of elevated levels of iron and copper

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Direct detection and quantification of transition metal ions in human atherosclerotic plaques : evidence for the presence of elevated levels of iron and copper. / Stadler, Nadina; Lindner, Robyn A; Davies, Michael Jonathan.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 24, No. 5, 2004, p. 949-54.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Stadler, N, Lindner, RA & Davies, MJ 2004, 'Direct detection and quantification of transition metal ions in human atherosclerotic plaques: evidence for the presence of elevated levels of iron and copper', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 24, no. 5, pp. 949-54. https://doi.org/10.1161/01.ATV.0000124892.90999.cb

APA

Stadler, N., Lindner, R. A., & Davies, M. J. (2004). Direct detection and quantification of transition metal ions in human atherosclerotic plaques: evidence for the presence of elevated levels of iron and copper. Arteriosclerosis, Thrombosis, and Vascular Biology, 24(5), 949-54. https://doi.org/10.1161/01.ATV.0000124892.90999.cb

Vancouver

Stadler N, Lindner RA, Davies MJ. Direct detection and quantification of transition metal ions in human atherosclerotic plaques: evidence for the presence of elevated levels of iron and copper. Arteriosclerosis, Thrombosis, and Vascular Biology. 2004;24(5):949-54. https://doi.org/10.1161/01.ATV.0000124892.90999.cb

Author

Stadler, Nadina ; Lindner, Robyn A ; Davies, Michael Jonathan. / Direct detection and quantification of transition metal ions in human atherosclerotic plaques : evidence for the presence of elevated levels of iron and copper. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2004 ; Vol. 24, No. 5. pp. 949-54.

Bibtex

@article{6ff422ec86c544c08916fad0dfdbe0ce,
title = "Direct detection and quantification of transition metal ions in human atherosclerotic plaques: evidence for the presence of elevated levels of iron and copper",
abstract = "OBJECTIVE: The involvement of transition metals in atherosclerosis is controversial. Some epidemiological studies have reported a relationship between iron (Fe) and cardiovascular disease, whereas others have not. Experimental studies have reported elevated levels of iron and copper (Cu) in diseased human arteries but have often used methods that release metal ions from proteins.METHODS AND RESULTS: In this study, we have used the minimally invasive technique of electron paramagnetic resonance (EPR) spectroscopy and inductively coupled plasma mass spectroscopy (ICPMS) to quantify iron and copper in ex vivo healthy human arteries and carotid lesions. The EPR spectra detected are characteristic of nonheme Fe(III) complexes. Statistically elevated levels of iron were detected in the intima of lesions compared with healthy controls (0.370 versus 0.022 nmol/mg tissue for EPR, 0.525 versus 0.168 nmol/mg tissue by ICPMS, P<0.05 in each cases). Elevated levels of copper were also detected (7.51 versus 2.01 pmol/mg tissue, lesion versus healthy control, respectively, P<0.05). Iron levels did not correlate with the gender or age of the donor, or tissue protein or calcium levels, but cholesterol levels correlated positively with iron accumulation, as measured by EPR.CONCLUSIONS: These data support the hypothesis that iron accumulates in human lesions and may contribute to disease progression.",
keywords = "Aged, Aged, 80 and over, Aorta, Carotid Arteries, Carotid Artery Diseases, Cholesterol, Copper, Coronary Artery Bypass, Electron Spin Resonance Spectroscopy, Endarterectomy, Carotid, Female, Humans, Iron, Male, Mammary Arteries, Mass Spectrometry, Middle Aged, Radial Artery, Tunica Intima",
author = "Nadina Stadler and Lindner, {Robyn A} and Davies, {Michael Jonathan}",
year = "2004",
doi = "10.1161/01.ATV.0000124892.90999.cb",
language = "English",
volume = "24",
pages = "949--54",
journal = "Arteriosclerosis, Thrombosis, and Vascular Biology",
issn = "1079-5642",
publisher = "Lippincott Williams & Wilkins",
number = "5",

}

RIS

TY - JOUR

T1 - Direct detection and quantification of transition metal ions in human atherosclerotic plaques

T2 - evidence for the presence of elevated levels of iron and copper

AU - Stadler, Nadina

AU - Lindner, Robyn A

AU - Davies, Michael Jonathan

PY - 2004

Y1 - 2004

N2 - OBJECTIVE: The involvement of transition metals in atherosclerosis is controversial. Some epidemiological studies have reported a relationship between iron (Fe) and cardiovascular disease, whereas others have not. Experimental studies have reported elevated levels of iron and copper (Cu) in diseased human arteries but have often used methods that release metal ions from proteins.METHODS AND RESULTS: In this study, we have used the minimally invasive technique of electron paramagnetic resonance (EPR) spectroscopy and inductively coupled plasma mass spectroscopy (ICPMS) to quantify iron and copper in ex vivo healthy human arteries and carotid lesions. The EPR spectra detected are characteristic of nonheme Fe(III) complexes. Statistically elevated levels of iron were detected in the intima of lesions compared with healthy controls (0.370 versus 0.022 nmol/mg tissue for EPR, 0.525 versus 0.168 nmol/mg tissue by ICPMS, P<0.05 in each cases). Elevated levels of copper were also detected (7.51 versus 2.01 pmol/mg tissue, lesion versus healthy control, respectively, P<0.05). Iron levels did not correlate with the gender or age of the donor, or tissue protein or calcium levels, but cholesterol levels correlated positively with iron accumulation, as measured by EPR.CONCLUSIONS: These data support the hypothesis that iron accumulates in human lesions and may contribute to disease progression.

AB - OBJECTIVE: The involvement of transition metals in atherosclerosis is controversial. Some epidemiological studies have reported a relationship between iron (Fe) and cardiovascular disease, whereas others have not. Experimental studies have reported elevated levels of iron and copper (Cu) in diseased human arteries but have often used methods that release metal ions from proteins.METHODS AND RESULTS: In this study, we have used the minimally invasive technique of electron paramagnetic resonance (EPR) spectroscopy and inductively coupled plasma mass spectroscopy (ICPMS) to quantify iron and copper in ex vivo healthy human arteries and carotid lesions. The EPR spectra detected are characteristic of nonheme Fe(III) complexes. Statistically elevated levels of iron were detected in the intima of lesions compared with healthy controls (0.370 versus 0.022 nmol/mg tissue for EPR, 0.525 versus 0.168 nmol/mg tissue by ICPMS, P<0.05 in each cases). Elevated levels of copper were also detected (7.51 versus 2.01 pmol/mg tissue, lesion versus healthy control, respectively, P<0.05). Iron levels did not correlate with the gender or age of the donor, or tissue protein or calcium levels, but cholesterol levels correlated positively with iron accumulation, as measured by EPR.CONCLUSIONS: These data support the hypothesis that iron accumulates in human lesions and may contribute to disease progression.

KW - Aged

KW - Aged, 80 and over

KW - Aorta

KW - Carotid Arteries

KW - Carotid Artery Diseases

KW - Cholesterol

KW - Copper

KW - Coronary Artery Bypass

KW - Electron Spin Resonance Spectroscopy

KW - Endarterectomy, Carotid

KW - Female

KW - Humans

KW - Iron

KW - Male

KW - Mammary Arteries

KW - Mass Spectrometry

KW - Middle Aged

KW - Radial Artery

KW - Tunica Intima

U2 - 10.1161/01.ATV.0000124892.90999.cb

DO - 10.1161/01.ATV.0000124892.90999.cb

M3 - Journal article

VL - 24

SP - 949

EP - 954

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

SN - 1079-5642

IS - 5

ER -

ID: 138274316