Determination and validation of peak fat oxidation in endurance-trained men using an upper body graded exercise test

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Peak fat oxidation rate (PFO) and the intensity that elicits PFO (Fat(max)) are commonly determined by a validated graded exercise test (GE) on a cycling ergometer with indirect calorimetry. However, for upper body exercise fat oxidation rates are not well elucidated and no protocol has been validated. Thus, our aim was to test validity and inter-method reliability for determination of PFO and Fat(max) in trained men using a GE protocol applying double poling on a ski-ergometer. PFO and Fat(max) were assessed during two identical GE tests (GE1 and GE2) and validated against separated short continuous exercise bouts (SCE) at 35%, 50%, and 65% of V?O-2peak on the ski-ergometer in 10 endurance-trained men (V?O-2peak: 65.1 +/- 1.0 mL center dot min(-1)center dot kg(-1), mean +/- SEM). Between GE tests no differences were found in PFO (GE1: 0.42 +/- 0.03; GE2: 0.45 +/- 0.03 g center dot min(-1), P = .256) or Fat(max) (GE1: 41 +/- 2%; GE2: 43 +/- 3% of V?O-2peak, P = .457) and the intra-individual coefficient of variation (CV) was 8 +/- 2% and 11 +/- 2% for PFO and Fat(max), respectively. Between GE and SCE tests, PFO (GE(avg): 0.44 +/- 0.03; SCE; 0.47 +/- 0.06 g center dot min(-1), P = .510) was not different, whereas a difference in Fat(max) (GE(avg): 42 +/- 2%; SCE: 52 +/- 4% of V?O-2peak, P = .030) was observed with a CV of 17 +/- 4% and 15 +/- 4% for PFO and Fat(max), respectively. In conclusion, GE has a high day-to-day reliability in determination of PFO and Fat(max) in trained men, whereas it is unclear if PFO and Fat(max) determined by GE reflect continuous exercise in general.
Original languageEnglish
JournalScandinavian Journal of Medicine & Science in Sports
ISSN0905-7188
DOIs
Publication statusE-pub ahead of print - 2019

    Research areas

  • exercise testing, Fat(max), lipid metabolism, peak fat oxidation, test reliability, test validity, upper body exercise

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