Degradation of extracellular matrix by peroxynitrite/peroxynitrous acid

Research output: Contribution to journalJournal articleResearchpeer-review

Eleanor C Kennett, Michael Jonathan Davies

The extracellular matrix (ECM) provides strength and elasticity to tissues and plays a key role in regulating cell behavior; damage to this material is believed to be a major factor in many inflammatory diseases. Peroxynitrite/peroxynitrous acid, which is generated at elevated levels at sites of inflammation, is believed to play a role in ECM damage; however, the mechanisms involved are poorly understood. Here we examined the reactions of bolus peroxynitrite, and that generated in a time-dependent manner by SIN-1 decomposition, with ECM isolated from a vascular smooth muscle cell line and porcine thoracic aorta. Bolus peroxynitrite caused the release of ECM glycosaminoglycans and proteins, the formation of 3-nitroTyr, and the detection of ECM-derived radicals (by immuno-spin trapping) in a concentration-dependent manner. Release and nitration of ECM components were modulated by the local pH and bicarbonate. SIN-1 caused the release of glycosaminoglycan, but not protein, from vascular smooth muscle cell-derived ECM in a concentration-, time-, and pH-dependent manner. The data presented here suggest that peroxynitrite-mediated damage to ECM occurs via a radical-mediated pathway. These reactions may contribute to ECM damage at sites of inflammation and play a role in disease progression, including rupture of atherosclerotic lesions.

Original languageEnglish
JournalFree Radical Biology & Medicine
Volume45
Issue number5
Pages (from-to)716-25
Number of pages10
ISSN0891-5849
DOIs
Publication statusPublished - 1 Sep 2008
Externally publishedYes

    Research areas

  • Animals, Bicarbonates, Cells, Cultured, Extracellular Matrix, Hydrogen-Ion Concentration, Molecular Structure, Molsidomine, Muscle, Smooth, Vascular, Myocytes, Smooth Muscle, Peroxynitrous Acid, Rats, Swine, Tyrosine

ID: 129670781