Biochemical and Mass Spectrometry-Based Approaches to Profile SUMOylation in Human Cells

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Biochemical and Mass Spectrometry-Based Approaches to Profile SUMOylation in Human Cells. / Kessler, Benedikt M; Bursomanno, Sara; McGouran, Joanna F; Hickson, Ian D; Liu, Ying.

Activity-Based Proteomics: Methods and Protocols. ed. / Herman S. Overkleeft; Bogdan I. Florea. Vol. 1491 Humana Press, 2017. p. 131-144 (Methods in Molecular Biology, Vol. 1491).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Kessler, BM, Bursomanno, S, McGouran, JF, Hickson, ID & Liu, Y 2017, Biochemical and Mass Spectrometry-Based Approaches to Profile SUMOylation in Human Cells. in HS Overkleeft & BI Florea (eds), Activity-Based Proteomics: Methods and Protocols. vol. 1491, Humana Press, Methods in Molecular Biology, vol. 1491, pp. 131-144. https://doi.org/10.1007/978-1-4939-6439-0_10

APA

Kessler, B. M., Bursomanno, S., McGouran, J. F., Hickson, I. D., & Liu, Y. (2017). Biochemical and Mass Spectrometry-Based Approaches to Profile SUMOylation in Human Cells. In H. S. Overkleeft, & B. I. Florea (Eds.), Activity-Based Proteomics: Methods and Protocols (Vol. 1491, pp. 131-144). Humana Press. Methods in Molecular Biology Vol. 1491 https://doi.org/10.1007/978-1-4939-6439-0_10

Vancouver

Kessler BM, Bursomanno S, McGouran JF, Hickson ID, Liu Y. Biochemical and Mass Spectrometry-Based Approaches to Profile SUMOylation in Human Cells. In Overkleeft HS, Florea BI, editors, Activity-Based Proteomics: Methods and Protocols. Vol. 1491. Humana Press. 2017. p. 131-144. (Methods in Molecular Biology, Vol. 1491). https://doi.org/10.1007/978-1-4939-6439-0_10

Author

Kessler, Benedikt M ; Bursomanno, Sara ; McGouran, Joanna F ; Hickson, Ian D ; Liu, Ying. / Biochemical and Mass Spectrometry-Based Approaches to Profile SUMOylation in Human Cells. Activity-Based Proteomics: Methods and Protocols. editor / Herman S. Overkleeft ; Bogdan I. Florea. Vol. 1491 Humana Press, 2017. pp. 131-144 (Methods in Molecular Biology, Vol. 1491).

Bibtex

@inbook{72efbe5afff24e2e8826ee4fe8b98a58,
title = "Biochemical and Mass Spectrometry-Based Approaches to Profile SUMOylation in Human Cells",
abstract = "Posttranslational modification of proteins with the small ubiquitin-like modifier (SUMO) regulates protein function in the context of cell cycle and DNA repair. The occurrence of SUMOylation is less frequent as compared to protein modification with ubiquitin, and appears to be controlled by a smaller pool of conjugating and deconjugating enzymes. Mass spectrometry has been instrumental in defining specific as well as proteome-wide views of SUMO-dependent biological processes, and several methodological approaches have been developed in the recent past. Here, we provide an overview of the latest experimental approaches to the study of SUMOylation, and also describe hands-on protocols using a combination of biochemistry and mass spectrometry-based technologies to profile proteins that are SUMOylated in human cells.",
author = "Kessler, {Benedikt M} and Sara Bursomanno and McGouran, {Joanna F} and Hickson, {Ian D} and Ying Liu",
year = "2017",
doi = "10.1007/978-1-4939-6439-0_10",
language = "English",
isbn = "978-1-4939-6437-6",
volume = "1491",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "131--144",
editor = "Overkleeft, {Herman S.} and Florea, {Bogdan I.}",
booktitle = "Activity-Based Proteomics",
address = "United States",

}

RIS

TY - CHAP

T1 - Biochemical and Mass Spectrometry-Based Approaches to Profile SUMOylation in Human Cells

AU - Kessler, Benedikt M

AU - Bursomanno, Sara

AU - McGouran, Joanna F

AU - Hickson, Ian D

AU - Liu, Ying

PY - 2017

Y1 - 2017

N2 - Posttranslational modification of proteins with the small ubiquitin-like modifier (SUMO) regulates protein function in the context of cell cycle and DNA repair. The occurrence of SUMOylation is less frequent as compared to protein modification with ubiquitin, and appears to be controlled by a smaller pool of conjugating and deconjugating enzymes. Mass spectrometry has been instrumental in defining specific as well as proteome-wide views of SUMO-dependent biological processes, and several methodological approaches have been developed in the recent past. Here, we provide an overview of the latest experimental approaches to the study of SUMOylation, and also describe hands-on protocols using a combination of biochemistry and mass spectrometry-based technologies to profile proteins that are SUMOylated in human cells.

AB - Posttranslational modification of proteins with the small ubiquitin-like modifier (SUMO) regulates protein function in the context of cell cycle and DNA repair. The occurrence of SUMOylation is less frequent as compared to protein modification with ubiquitin, and appears to be controlled by a smaller pool of conjugating and deconjugating enzymes. Mass spectrometry has been instrumental in defining specific as well as proteome-wide views of SUMO-dependent biological processes, and several methodological approaches have been developed in the recent past. Here, we provide an overview of the latest experimental approaches to the study of SUMOylation, and also describe hands-on protocols using a combination of biochemistry and mass spectrometry-based technologies to profile proteins that are SUMOylated in human cells.

U2 - 10.1007/978-1-4939-6439-0_10

DO - 10.1007/978-1-4939-6439-0_10

M3 - Book chapter

C2 - 27778286

SN - 978-1-4939-6437-6

VL - 1491

T3 - Methods in Molecular Biology

SP - 131

EP - 144

BT - Activity-Based Proteomics

A2 - Overkleeft, Herman S.

A2 - Florea, Bogdan I.

PB - Humana Press

ER -

ID: 169993150