Basement membrane chondroitin sulfate proteoglycans: localization in adult rat tissues.

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K J McCarthy, J R Couchman

Heparan sulfate proteoglycans have been described as the major proteoglycan component of basement membranes. However, previous investigators have also provided evidence for the presence of chondroitin sulfate glycosaminoglycan in these structures. Recently we described the production and characterization of core protein-specific monoclonal antibodies (MAb) against a chondroitin sulfate proteoglycan (CSPG) present in Reichert's membrane, a transient extra-embryonic structure of rodents. This CSPG was also demonstrated to be present in adult rat kidney. We report here the tissue distribution of epitopes recognized by these MAb. The ubiquitous presence of these epitopes in the basement membranes of nearly all adult rat tissues demonstrates that at least one CSPG is a constituent of most basement membranes, and by virtue of its unique distribution is distinct from other chondroitin and dermatan sulfate proteoglycans previously described.
Original languageEnglish
JournalJournal of Histochemistry and Cytochemistry
Volume38
Issue number10
Pages (from-to)1479-86
Number of pages7
ISSN0022-1554
Publication statusPublished - 1990

Bibliographical note

Keywords: Animals; Antibodies, Monoclonal; Basement Membrane; Female; Immunohistochemistry; Male; Microscopy, Electron; Proteochondroitin Sulfates; Proteoglycans; Rats; Rats, Inbred Strains

ID: 5166634