Basement membrane and interstitial proteoglycans produced by MDCK cells correspond to those expressed in the kidney cortex.
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Basement membrane and interstitial proteoglycans produced by MDCK cells correspond to those expressed in the kidney cortex. / Erickson, A C; Couchman, J R.
In: Matrix Biology, Vol. 19, No. 8, 2001, p. 769-78.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Basement membrane and interstitial proteoglycans produced by MDCK cells correspond to those expressed in the kidney cortex.
AU - Erickson, A C
AU - Couchman, J R
N1 - Keywords: Amino Acid Sequence; Animals; Basement Membrane; Cell Line; Chondroitin ABC Lyase; Chondroitin Sulfates; Collagen; Dermatan Sulfate; Dogs; Extracellular Space; Heparan Sulfate Proteoglycans; Heparitin Sulfate; Humans; Kidney Cortex; Mice; Molecular Sequence Data; Polysaccharide-Lyases; Proteoglycans; Rats
PY - 2001
Y1 - 2001
N2 - Multiple proteoglycans (PGs) are present in all basement membranes (BM) and may contribute to their structure and function, but their effects on cell behavior are not well understood. Their postulated functions include: a structural role in maintaining tissue histoarchitecture, or aid in selective filtration processes; sequestration of growth factors; and regulation of cellular differentiation. Furthermore, expression PGs has been found to vary in several disease states. In order to elucidate the role of PGs in the BM, a well-characterized model of polarized epithelium, Madin-Darby canine kidney (MDCK) cells has been utilized. Proteoglycans were prepared from conditioned medium by DEAE anion exchange chromatography. The eluted PGs were treated with heparitinase or chondroitinase ABC (cABC), separately or combined, followed by SDS-PAGE. Western blot analysis, using antibodies specific for various PG core proteins or CS stubs generated by cABC treatment, revealed that both basement membrane and interstitial PGs are secreted by MDCK cells. HSPGs expressed by MDCK cells are perlecan, agrin, and collagen XVIII. Various CSPG core proteins are made by MDCK cells and have been identified as biglycan, bamacan, and versican (PG-M). These PGs are also associated with mammalian kidney tubules in vivo.
AB - Multiple proteoglycans (PGs) are present in all basement membranes (BM) and may contribute to their structure and function, but their effects on cell behavior are not well understood. Their postulated functions include: a structural role in maintaining tissue histoarchitecture, or aid in selective filtration processes; sequestration of growth factors; and regulation of cellular differentiation. Furthermore, expression PGs has been found to vary in several disease states. In order to elucidate the role of PGs in the BM, a well-characterized model of polarized epithelium, Madin-Darby canine kidney (MDCK) cells has been utilized. Proteoglycans were prepared from conditioned medium by DEAE anion exchange chromatography. The eluted PGs were treated with heparitinase or chondroitinase ABC (cABC), separately or combined, followed by SDS-PAGE. Western blot analysis, using antibodies specific for various PG core proteins or CS stubs generated by cABC treatment, revealed that both basement membrane and interstitial PGs are secreted by MDCK cells. HSPGs expressed by MDCK cells are perlecan, agrin, and collagen XVIII. Various CSPG core proteins are made by MDCK cells and have been identified as biglycan, bamacan, and versican (PG-M). These PGs are also associated with mammalian kidney tubules in vivo.
M3 - Journal article
C2 - 11223336
VL - 19
SP - 769
EP - 778
JO - Matrix Biology
JF - Matrix Biology
SN - 0945-053X
IS - 8
ER -
ID: 5163258